Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F44 I1 R1
|
19 |
65.8 |
3201541 |
96.2% |
3079882 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
756,180 |
G→T |
G92W (GGG→TGG) |
sdhA → |
succinate dehydrogenase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 756,180 | 0 | G | T | 94.1%
| 222.5
/ 1.9
| 68 | G92W (GGG→TGG) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base T (36/28); total (38/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCG > NC_000913/756085‑756275
|
cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc > 2:1380822/1‑100 (MQ=255)
ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg < 1:274834/100‑1 (MQ=255)
ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg > 1:645636/1‑100 (MQ=255)
ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg > 1:552801/1‑100 (MQ=255)
cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg > 2:1559464/1‑100 (MQ=255)
tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa > 2:70469/1‑100 (MQ=255)
tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa > 2:1584335/1‑100 (MQ=255)
gATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATGGAATATATGTGTAAAACCTGGCCGGAAg > 2:1502735/1‑97 (MQ=255)
tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGAtt > 2:37070/1‑100 (MQ=255)
aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt > 1:901253/1‑100 (MQ=255)
cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg > 2:1415200/1‑100 (MQ=255)
gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa < 1:51996/100‑1 (MQ=255)
gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa < 2:1449151/100‑1 (MQ=255)
ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGaa < 1:403889/100‑1 (MQ=255)
gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc > 1:732965/1‑100 (MQ=255)
tGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCg < 2:1046484/100‑1 (MQ=255)
ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa > 2:1588078/1‑100 (MQ=255)
gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGaa < 2:999956/100‑1 (MQ=255)
cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac > 2:1001431/1‑100 (MQ=255)
cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac < 2:650201/100‑1 (MQ=255)
tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac < 2:1442684/100‑1 (MQ=255)
tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac < 2:637758/100‑1 (MQ=255)
atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca > 2:1107375/1‑100 (MQ=255)
atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca > 1:1361043/1‑100 (MQ=255)
tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACAt > 2:1497508/1‑100 (MQ=255)
tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACAt > 2:1522051/1‑100 (MQ=255)
aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg < 2:1008429/100‑1 (MQ=255)
gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc > 2:668016/1‑100 (MQ=255)
acacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt > 1:615351/1‑100 (MQ=255)
cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg > 2:438186/1‑100 (MQ=255)
ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc > 1:393338/1‑100 (MQ=255)
gAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc < 2:672515/100‑1 (MQ=255)
aGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg < 2:552805/100‑1 (MQ=255)
aGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg < 1:801362/100‑1 (MQ=255)
gggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgc > 1:1057736/1‑100 (MQ=255)
ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg < 1:402491/100‑1 (MQ=255)
gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt > 1:139401/1‑100 (MQ=255)
gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt > 1:404417/1‑100 (MQ=255)
tCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtc > 2:497582/1‑100 (MQ=255)
ggACTATATCGGTGACCAGGACGCGATTGCATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGATCTCGAACACATGGGCCTGCCGTTCTCGCGtctc < 1:958469/100‑1 (MQ=255)
ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc > 1:1514732/1‑100 (MQ=255)
cTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat < 1:1395380/100‑1 (MQ=255)
atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatgat < 1:1415186/99‑1 (MQ=255)
gTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:1007713/100‑1 (MQ=255)
gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta < 1:1192930/100‑1 (MQ=255)
gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta < 1:1330551/100‑1 (MQ=255)
ccAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc > 1:1062045/1‑100 (MQ=255)
cAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct > 1:1159146/1‑100 (MQ=255)
ggACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTAGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta < 1:539457/99‑1 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 2:811202/100‑1 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGATCGtatctatc < 1:895248/100‑1 (MQ=255)
gACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc < 1:1588063/100‑1 (MQ=255)
aCGCGATTGATTATATGTGTAAGACCGGGCCGGTAGTGATTCTGTAACTAAAACCCATGTGTCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta < 1:601566/97‑1 (MQ=255)
aCGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCa < 1:454317/100‑1 (MQ=255)
cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa < 1:1001421/100‑1 (MQ=255)
cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa < 2:108254/100‑1 (MQ=255)
cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg > 2:21960/1‑100 (MQ=255)
cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg < 2:740877/100‑1 (MQ=255)
gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt > 2:366360/1‑100 (MQ=255)
tatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg > 1:374026/1‑100 (MQ=255)
gtgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGt > 1:619850/1‑100 (MQ=255)
tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc < 2:901260/100‑1 (MQ=255)
gtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa > 2:622097/1‑100 (MQ=255)
tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg > 1:1498147/1‑100 (MQ=255)
tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg > 1:1591848/1‑100 (MQ=255)
tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg > 1:951404/1‑100 (MQ=255)
aaaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt > 2:952256/1‑100 (MQ=255)
aaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCg > 1:564103/1‑100 (MQ=255)
|
CCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCG > NC_000913/756085‑756275
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
