Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F112 I1 R1
|
17 |
70.3 |
3408026 |
96.6% |
3292153 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
756,180 |
G→T |
G92W (GGG→TGG) |
sdhA → |
succinate dehydrogenase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 756,180 | 0 | G | T | 96.0%
| 245.4
/ ‑0.8
| 74 | G92W (GGG→TGG) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base T (41/30); total (41/33) |
| Fisher's exact test for biased strand distribution p-value = 8.42e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAG > NC_000913/756084‑756272
|
aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATCTGTAAAACCTGGc < 2:1157251/100‑1 (MQ=255)
cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc < 2:1510244/100‑1 (MQ=255)
cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc > 2:309687/1‑100 (MQ=255)
cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGGGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc > 2:320920/1‑100 (MQ=255)
ccATGAAGATAATTGGGAATGGCATATGTATGACACCGTTAAAGGGTCGGACTATATCGGTGACCAGGACGCTATTGAATATATGTGTAATACCTGGCCg < 1:469522/100‑1 (MQ=255)
cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg > 2:519137/1‑100 (MQ=255)
cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg > 2:244558/1‑100 (MQ=255)
aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCgg < 1:1618360/99‑1 (MQ=255)
tGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGaa > 2:1287408/1‑100 (MQ=255)
gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAg < 2:1635003/100‑1 (MQ=255)
aGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATAAATGTGTAAAACATGGCCGGAAGCg > 1:1681588/1‑100 (MQ=255)
tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGAtt < 2:169616/100‑1 (MQ=255)
aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt > 1:51257/1‑100 (MQ=255)
cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg > 2:1665292/1‑100 (MQ=255)
tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt < 1:1210446/98‑1 (MQ=255)
gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa < 1:519136/100‑1 (MQ=255)
gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa < 1:948319/100‑1 (MQ=255)
ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGaa > 1:162672/1‑100 (MQ=255)
aaTGGCATATATACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACt < 1:1114983/100‑1 (MQ=255)
aTGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGCACGCGATTGAATATATGTGTAAAACCTGGCCGGCCGCGATTCTGGAACTc > 1:1041598/1‑100 (MQ=255)
ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGTTGACCAGGCCGCGATTGAATATATGTGTAAAACCTGGCGGGACGCGATTCTggca > 1:1325206/1‑93 (MQ=255)
ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGa < 2:51257/100‑1 (MQ=255)
cATATGTACGACACCTGGAAATGGGCGGACTATATCTTTGACCAGGACTCGATTGAATATATTTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAac < 2:992733/100‑1 (MQ=255)
tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac > 1:1680815/1‑100 (MQ=255)
tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGGATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGCac > 1:712160/1‑96 (MQ=255)
gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATg > 1:906327/1‑100 (MQ=255)
aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg > 1:411045/1‑100 (MQ=255)
cGACACCGTGAAAGGGTCGGACTATATCGGTGACCATGTAGCGCTTGAAGATATGCGCAAACCCTGGCCGGAAGCAATTCTGGAACTCGACcaca > 1:1309528/1‑95 (MQ=255)
cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc > 2:292883/1‑100 (MQ=255)
cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc > 2:1088817/1‑100 (MQ=255)
cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg > 2:729869/1‑100 (MQ=255)
cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg > 2:1177428/1‑100 (MQ=255)
gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt > 2:892970/1‑100 (MQ=255)
gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt > 1:288700/1‑100 (MQ=255)
gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt < 2:1069348/100‑1 (MQ=255)
gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATAGGCCTGCCGt > 1:221106/1‑100 (MQ=255)
ggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg > 2:857591/1‑100 (MQ=255)
gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt > 2:1355960/1‑100 (MQ=255)
gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCg < 1:1074689/100‑1 (MQ=255)
cTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat > 1:40583/1‑100 (MQ=255)
tataTCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatg < 1:676651/100‑1 (MQ=255)
tataTCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatg < 2:1681595/100‑1 (MQ=255)
atatCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACTCATGGGCCTGCCGTTCTCGCGTCTCgatga < 1:553984/100‑1 (MQ=255)
tCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg < 2:363698/100‑1 (MQ=255)
cGGTGACCAGGACGCGATTGAAAAAAAAAGTAAAACCTGGCCGGAAGCGATTCTGGAACTAGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt > 1:733327/1‑100 (MQ=255)
ggTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc > 1:1581456/1‑100 (MQ=255)
gTGACCAGGACGCGATTGAATATATGTGTAAAACCTTGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg < 2:1193812/100‑1 (MQ=255)
gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta > 1:468119/1‑100 (MQ=255)
ccAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc > 1:1293503/1‑100 (MQ=255)
ccAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc > 1:623319/1‑100 (MQ=255)
cAGGACGCGATTGAATATATGTGTAAACCCGGGCCGGAAGCGATTCTGGAACTAGAACGCATGGGCCTGCCGTTCGCGCGTCTCGATGATGGTCGtatct < 1:169756/100‑1 (MQ=255)
cAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct < 1:913879/100‑1 (MQ=255)
aGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta < 2:843515/100‑1 (MQ=255)
ggACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat < 2:655545/100‑1 (MQ=255)
cgtgATTGAATATATGTGTAAGGTCTGGCCGGAAGCGTTTCTGTAACTCGAACACTTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCa < 1:296132/96‑1 (MQ=255)
cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa > 1:61272/1‑100 (MQ=255)
cgcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa < 2:954766/100‑1 (MQ=255)
gcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc < 2:65623/100‑1 (MQ=255)
gcgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc < 1:548282/100‑1 (MQ=255)
gATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGt > 1:794329/1‑100 (MQ=255)
aTTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc > 2:558215/1‑100 (MQ=255)
ttGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTcc > 2:671503/1‑100 (MQ=255)
tGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCg < 2:162672/100‑1 (MQ=255)
tGAATATATGTGGAATACCTGGCCTGAAGCGATTCTGGACTTCTAACATATGGGCCTGCCGTTCTCGCGTATCGATGATGGTCGTATCTATCAACGTCCg < 1:320919/100‑1 (MQ=255)
aaTATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt < 2:712162/100‑1 (MQ=255)
atatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt > 2:963085/1‑100 (MQ=255)
atatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg > 2:119789/1‑100 (MQ=255)
tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc > 2:828927/1‑100 (MQ=255)
tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc > 2:883712/1‑100 (MQ=255)
atGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg < 1:143625/100‑1 (MQ=255)
tgtgTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg < 2:592225/100‑1 (MQ=255)
gtgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGt > 2:375973/1‑100 (MQ=255)
tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc < 2:1549517/100‑1 (MQ=255)
tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg > 1:425778/1‑100 (MQ=255)
|
ACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAG > NC_000913/756084‑756272
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
