Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F44 I0 R1	58	156.1	7762724	94.5%	7335774	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	756,180	G→T	100%	G92W (GGG→TGG)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,180	0	G	T	98.9%	419.2 / ‑3.8	134	G92W (GGG→TGG)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base G (1/3); new base T (62/68); total (63/71)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGA  >  NC_000913/756083‑756276

  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGCCGCGCGTGAATATATGGGTAAAACCgg                                                                                                 >  1:1641752/1‑97 (MQ=255)

  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTACAACCTGGc                                                                                               >  1:3860277/1‑99 (MQ=255)

  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc                                                                                              <  2:2801610/100‑1 (MQ=255)

  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc                                                                                              >  1:3051263/1‑100 (MQ=255)

  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGcc                                                                                              <  1:2944950/100‑1 (MQ=255)

   ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCg                                                                                             <  1:1641681/100‑1 (MQ=255)

       gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGCTTGAATATATGAGTAAAACCTGGCCGGAAg                                                                                         >  1:2696365/1‑100 (MQ=255)

        aaGATGACTGGGAATGGCATATGTACGACACCGGGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGc                                                                                        <  2:2172/100‑1 (MQ=255)

         aGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGc                                                                                        <  1:3025878/99‑1 (MQ=255)

            tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGAtt                                                                                    >  2:1819837/1‑100 (MQ=255)

              aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGCTTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                  >  2:3444279/1‑100 (MQ=255)

              aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCt                                                                                  <  2:1863657/100‑1 (MQ=255)

               cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg                                                                                 >  1:1061383/1‑100 (MQ=255)

               cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg                                                                                 >  2:846003/1‑100 (MQ=255)

               cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTg                                                                                 <  2:2849330/100‑1 (MQ=255)

                tGGGAATGGCATATGTGCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTgg                                                                                >  2:2932542/1‑100 (MQ=255)

                tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTgg                                                                                <  2:1147598/100‑1 (MQ=255)

                 gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGa                                                                               <  2:667707/100‑1 (MQ=255)

                 gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGa                                                                               <  2:1359990/100‑1 (MQ=255)

                   gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                             <  2:3362725/100‑1 (MQ=255)

                   gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                             >  2:3125994/1‑100 (MQ=255)

                   gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAAc                                                                             <  2:2107557/100‑1 (MQ=255)

                      tGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCg                                                                          <  2:1298161/100‑1 (MQ=255)

                       ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGa                                                                         >  2:3838262/1‑100 (MQ=255)

                       ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGa                                                                         <  2:2017593/100‑1 (MQ=255)

                          atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAaca                                                                      <  1:1374255/100‑1 (MQ=255)

                           tatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAacac                                                                     <  1:3227379/100‑1 (MQ=255)

                            atGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAACCCTGGCCGGAAGCGATTCTGGAACTCGAAcaca                                                                    >  1:3868913/1‑100 (MQ=255)

                             tGTACGACACCGTGAAAGGGTCGGACTATATCTGTGTCCAGGACGTGATTGTATTTATGTGTAAAACTTGGCCGGATGCGATTCTGGTAATCGACCACAt                                                                   >  1:2127853/1‑100 (MQ=255)

                             tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAAcaca                                                                    >  1:3762142/1‑99 (MQ=255)

                             tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACAt                                                                   >  1:3840838/1‑100 (MQ=255)

                                aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                                <  2:2333594/100‑1 (MQ=255)

                                aCGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                                >  1:3494731/1‑100 (MQ=255)

                                 cGACACCGTGAAAGGGTCTGACTATATCGGTGACCAGGAAGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTATGGAACTCGAACACATggg                                                                <  1:3741280/99‑1 (MQ=255)

                                    nacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                            >  2:736405/2‑100 (MQ=255)

                                    cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                            <  2:2213458/100‑1 (MQ=255)

                                    cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                            <  1:543983/100‑1 (MQ=255)

                                     acCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                                                           >  1:1308792/1‑100 (MQ=255)

                                      ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                          >  1:3737384/1‑100 (MQ=255)

                                      ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                          >  1:3743026/1‑100 (MQ=255)

                                      ccGGGAAAGGGTCGGACTATATCGGGGACCAGGACGCGAATGAACATCGGGGGAGAACCTGGCCGGAAGAGATTATGCGACACGAACACATggg                                                                >  1:2149602/1‑94 (MQ=255)

                                       cGTGAAAGGGTCGGACTATATCGGTGACGAGGACGCGATTGAATATATTTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCg                                                         >  2:771042/1‑100 (MQ=255)

                                       cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                                                           <  1:238333/98‑1 (MQ=255)

                                       cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCg                                                         >  1:185943/1‑100 (MQ=255)

                                       cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCg                                                         <  2:3840870/100‑1 (MQ=255)

                                        gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt                                                        <  2:539397/100‑1 (MQ=255)

                                        gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATAGATGTGTAAAACCTGGCCGGAAGCGATTGTGGAACTCGAACACATGGGCCTGCCGt                                                        >  1:3017306/1‑100 (MQ=255)

                                            aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtctc                                                    <  2:54013/100‑1 (MQ=255)

                                            aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtctc                                                    <  2:143019/100‑1 (MQ=255)

                                              gggTCGGACTATCTAGGTGACCAGGTCGAGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACGTGGGCCTGCCGTTCTcg                                                   <  2:2510649/99‑1 (MQ=255)

                                               tgTCGGACTATATCGGTGACCAGGACGTGATTGAATATATGTGTAAAGCCTGGCCGGAAGCGATTGTGGAACTCGAACATATGGGCCTGCCGTGCTcgcg                                                 <  1:817356/99‑1 (MQ=255)

                                                   ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                             <  1:158439/100‑1 (MQ=255)

                                                   ggACTATATCGGTGACCAGGACGCGATTGAAAATATGTGTAAAACCTGGCCGGAAGCGGTTCTGGAACACGAACACATGGGCCTGCCGTTCTCGCGtctc                                             >  1:495431/1‑100 (MQ=255)

                                                    gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACATATGGGCCTGCCGTTCTCGCGTCTCg                                            <  1:69297/100‑1 (MQ=255)

                                                    gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCg                                            >  2:3387500/1‑100 (MQ=255)

                                                    gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCg                                            <  2:681540/100‑1 (MQ=255)

                                                    gACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCg                                            <  1:1934233/100‑1 (MQ=255)

                                                     aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGGTTATGGCACACGAACAAATGGGCCTGCGGTTCTCGCGTCCCga                                           >  1:3378215/1‑100 (MQ=255)

                                                      atggatCGGGGGCCGGGGCGCGATAGAGTATATGGGGAACAGCGCGCGGGAGGCGAGTCTGGAACTCGAACACAAGAGCCGGCTGTTCTGGCGTCCCgac                                          <  1:1726190/96‑2 (MQ=255)

                                                          atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATTGGCCTGCAGTTCTTGCGTCTCGatgatg                                      >  1:2958441/1‑100 (MQ=255)

                                                            cGGTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt                                    <  2:1641761/100‑1 (MQ=255)

                                                              gTGACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                  <  1:75996/100‑1 (MQ=255)

                                                                gACCAGGACGCGATTGAATATGTGTGCAAAACCTGGCCGGAAGCGATTCTGGAACTCTGACGCATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGt                                 <  1:3394222/99‑1 (MQ=255)

                                                                gACCAGGACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                <  2:1431632/100‑1 (MQ=255)

                                                                 actaGGACGCGATTGATTATATGTGTAAAAGCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCGGCCGTTCTCGCGTCTCGATGATGGTCGtat                               <  1:991419/97‑1 (MQ=255)

                                                                  ccAGGACGGGATTGAATATATGGGTAACACCTGGCCGGAAGCGATTCTGGAACTCGAGCGCATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                              <  1:916214/100‑1 (MQ=255)

                                                                    agaaagggcttGAATATATGGGTAACACCTGGCCGGAAGCGATTCTGGGACTAGAACGCATGGGTCTGACGTTCTCGCGTCTCGATGATGGTCGtatc                              <  1:2542074/89‑1 (MQ=255)

                                                                     ggACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                           >  1:1939924/1‑100 (MQ=255)

                                                                     ggACGCGATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGACGTTCTCGCGTCTCGATGATGGTCGtatctat                           <  2:3675396/100‑1 (MQ=255)

                                                                        cgcgATTGATTATATGTGTAAAACGTGGCAGGAAGCGATTCTGGAACTCGAACACAAGGGCCTGCCGTTATCGCGGGTCGATGATGGTCGGATCTATCaa                        <  1:729895/100‑1 (MQ=255)

                                                                        cgcgATTGAATATATGTGTAGAACATGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCGGACGTTCTCGCGTCTCGATGATGGTCGtatctatc                          <  1:1173140/98‑1 (MQ=255)

                                                                          cgATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                      >  1:2800993/1‑100 (MQ=255)

                                                                           gATTGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTATCGCGTCTCGATGATGGTCGTATCTATCAACGt                     >  1:934919/1‑100 (MQ=255)

                                                                             ttGAATATATGTGTAAAACCTGGGCGGAAGGGATTCTGGAATTCGAACACATGGGCCTGGCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTcc                   <  1:994990/100‑1 (MQ=255)

                                                                             ttGAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTcc                   >  2:2771625/1‑100 (MQ=255)

                                                                               gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCga                 >  2:3062401/1‑99 (MQ=255)

                                                                               gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                 >  1:927245/1‑100 (MQ=255)

                                                                               gAATATATGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                 >  2:1306218/1‑100 (MQ=255)

                                                                                  tatatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg              <  2:844335/100‑1 (MQ=255)

                                                                                    tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc            >  1:2140676/1‑100 (MQ=255)

                                                                                    tatGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc            >  2:75126/1‑100 (MQ=255)

                                                                                     atGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg           <  2:680267/100‑1 (MQ=255)

                                                                                     atGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg           <  2:1021436/100‑1 (MQ=255)

                                                                                     atGTGTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg           <  2:3674199/100‑1 (MQ=255)

                                                                                      tgtgTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg          >  2:3461669/1‑100 (MQ=255)

                                                                                      tgtgTAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCATATCTATCAACGTCCGTTTGGc            >  1:3666616/1‑98 (MQ=255)

                                                                                       gtgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg           >  1:1933844/1‑98 (MQ=255)

                                                                                        tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc        >  2:2671904/1‑100 (MQ=255)

                                                                                        tgtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCGCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg          <  1:73073/98‑1 (MQ=255)

                                                                                         gtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa       <  1:2951260/100‑1 (MQ=255)

                                                                                         gtAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa       >  1:419887/1‑100 (MQ=255)

                                                                                          tAAAACCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg      >  2:1757421/1‑100 (MQ=255)

                                                                                          tAAAACCGGGCCGGAAGCGATTCTGGATCTCGAGCACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTAACAACGTCCGTTTGGCGGTCAg      <  1:270256/100‑1 (MQ=255)

                                                                                           aaaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg      <  1:102763/99‑1 (MQ=255)

                                                                                           aaaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt     >  1:1079175/1‑100 (MQ=255)

                                                                                           aaaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGGATCTATCAACGTCCGTTTGGCGGTCAGt     >  1:3792890/1‑100 (MQ=255)

                                                                                            aaaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc    <  2:3529004/100‑1 (MQ=255)

                                                                                             aaCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCg   <  2:1164880/100‑1 (MQ=255)

                                                                                              aCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGa  <  2:3743057/100‑1 (MQ=255)

                                                                                              aCCTGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGa  <  2:1753383/100‑1 (MQ=255)

TACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGA  >  NC_000913/756083‑756276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A