Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F200 I0 R1 101 105.4 5209919 94.4% 4918163 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,249,628 G→A 80.9% G85D (GGC→GAC)  yqjD → membrane‑anchored ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,249,6280GA80.9% 264.2 / 57.7 126G85D (GGC→GAC) yqjDmembrane‑anchored ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (10/14);  new base A (42/60);  total (52/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 126 total aligned reads displayed.

GCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGT  >  NC_000913/3249532‑3249723
                                                                                                |                                                                                               
gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCAt                                                                                              >  1:2459856/1‑100 (MQ=255)
gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAt                                                                                              >  1:218401/1‑100 (MQ=255)
gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAt                                                                                              >  2:1282129/1‑100 (MQ=255)
   tGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTgg                                                                                           <  2:2459843/100‑1 (MQ=255)
    gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGACGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgc                                                                                          <  2:249562/100‑1 (MQ=255)
     ggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcg                                                                                         >  1:515899/1‑100 (MQ=255)
     ggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcg                                                                                         >  1:356265/1‑100 (MQ=255)
      gTGAAACCGGTGATGCCATTGCCAAACAATCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCCCGAAAATCCGTGGACGGGCGTGGACATTGgcgc                                                                                        <  2:1562181/100‑1 (MQ=255)
      gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcgc                                                                                        >  2:1728215/1‑100 (MQ=255)
       tGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCt                                                                                       >  2:2386354/1‑100 (MQ=255)
       tGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCt                                                                                       >  1:2151252/1‑100 (MQ=255)
       tGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCt                                                                                       <  1:1445197/100‑1 (MQ=255)
        gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                      <  2:257827/100‑1 (MQ=255)
        gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                      >  1:2164185/1‑100 (MQ=255)
         aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTg                                                                                      <  2:241882/99‑1 (MQ=255)
         aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGc                                                                                     <  2:1450697/100‑1 (MQ=255)
          aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCa                                                                                    >  1:545999/1‑100 (MQ=255)
           aCCGGTGATGCCATTGTCAAACAATCCCGTGTTGCGGCGGCGCGTGCCGAGGAGCATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCaa                                                                                   <  1:123119/100‑1 (MQ=255)
              ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGGTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCg                                                                                <  2:2583804/100‑1 (MQ=255)
               gTGATGCCATTGCCAAACAATCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTTCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                               <  1:1150146/100‑1 (MQ=255)
                 gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGgtg                                                                             <  1:2057425/100‑1 (MQ=255)
                 gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                             >  1:2486396/1‑100 (MQ=255)
                 gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                             <  2:1617152/100‑1 (MQ=255)
                 gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                             <  2:755128/100‑1 (MQ=255)
                   tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGgtg                                                                             <  2:1767576/98‑1 (MQ=255)
                   tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                           >  2:1486773/1‑100 (MQ=255)
                     ccATTGCCAAACAAACCCGTGTCGCGGCGGGGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                          <  2:1213684/99‑1 (MQ=255)
                     ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                         >  1:196470/1‑100 (MQ=255)
                     ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                         <  2:1892909/100‑1 (MQ=255)
                     ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                         >  2:1529068/1‑100 (MQ=255)
                        ttGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCt                                                                      <  1:1163911/100‑1 (MQ=255)
                         tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                     >  1:1138317/1‑100 (MQ=255)
                         tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                     <  2:294744/100‑1 (MQ=255)
                          gCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                    <  1:632882/100‑1 (MQ=255)
                          gCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                    >  2:1593230/1‑100 (MQ=255)
                            caaacaaaCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                    <  2:1787775/98‑1 (MQ=255)
                            caaacaaaCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                  >  1:2251813/1‑100 (MQ=255)
                             aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                 <  1:1272282/100‑1 (MQ=255)
                             aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                 <  1:2212259/100‑1 (MQ=255)
                              aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                >  1:1200768/1‑100 (MQ=255)
                              aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                <  2:628150/100‑1 (MQ=255)
                                caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                              <  1:2443835/100‑1 (MQ=255)
                                 aaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTct                                                             <  1:44994/100‑1 (MQ=255)
                                   acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  2:440809/100‑1 (MQ=255)
                                   acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                           <  1:177547/100‑1 (MQ=255)
                                    cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgct                                                          <  2:220368/100‑1 (MQ=255)
                                     ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGATCGGCGTTctgctg                                                         >  2:93388/1‑100 (MQ=255)
                                        tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGATATTGGCGCTGTAATCGGTGTAGTGCTCGCCGTTCTGCTGtag                                                      <  1:1758827/100‑3 (MQ=255)
                                        tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                      >  1:1149828/1‑100 (MQ=255)
                                        tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                      <  2:255660/100‑1 (MQ=255)
                                          tCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcgcg                                                    <  1:2333060/100‑1 (MQ=255)
                                          tCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcgcg                                                    <  1:2054718/100‑1 (MQ=255)
                                           cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcgcg                                                    >  2:1586639/1‑99 (MQ=255)
                                           cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                   >  1:2248715/1‑100 (MQ=255)
                                           cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGGGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                   <  1:159247/100‑1 (MQ=255)
                                              ggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtcgt                                                <  1:611655/100‑1 (MQ=255)
                                               gcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGtt                                               <  2:47026/100‑1 (MQ=255)
                                                cggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTa                                              >  1:1040043/1‑100 (MQ=255)
                                                 ggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTaa                                             <  2:1787340/100‑1 (MQ=255)
                                                  gcgcGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAAt                                            <  2:514038/100‑1 (MQ=255)
                                                  gcgcGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAAt                                            <  1:2101740/100‑1 (MQ=255)
                                                   cgcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAAtt                                           >  1:1687344/1‑100 (MQ=255)
                                                    gcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTa                                          <  1:1600559/100‑1 (MQ=255)
                                                    gcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTa                                          <  1:339603/100‑1 (MQ=255)
                                                      gTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                        >  2:1847799/1‑100 (MQ=255)
                                                      gTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                        >  2:1909087/1‑100 (MQ=255)
                                                        gCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGc                                      <  1:1507553/100‑1 (MQ=255)
                                                        gCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGGTCTGCTGTCGCGTCGTTAATTATGGc                                      >  2:82544/1‑100 (MQ=255)
                                                         ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                     >  1:1462596/1‑100 (MQ=255)
                                                         ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                     >  1:107599/1‑100 (MQ=255)
                                                          cGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                    <  2:846504/100‑1 (MQ=255)
                                                            aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                    <  2:957077/98‑1 (MQ=255)
                                                            aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                  <  1:525845/100‑1 (MQ=255)
                                                               aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                               <  2:728245/100‑1 (MQ=255)
                                                                gTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtc                              >  2:1939506/1‑100 (MQ=255)
                                                                 tATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtca                             <  1:1036698/100‑1 (MQ=255)
                                                                    gtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCTTCGTTAATTATGGCGGACACtcatca                          <  1:1585864/100‑1 (MQ=255)
                                                                    gtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                          >  1:81207/1‑100 (MQ=255)
                                                                    gtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                          >  1:645206/1‑100 (MQ=255)
                                                                    gtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                          >  1:1834512/1‑100 (MQ=255)
                                                                     tgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCAc                         <  1:2590200/100‑1 (MQ=255)
                                                                     tgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGATAATTAAGGCGGACACTCATCAc                         >  2:1322525/1‑100 (MQ=255)
                                                                     tgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGGTCGCCGGTCGCGTCGATAATTAAGGCGGACACtcatca                          >  2:1616430/1‑99 (MQ=255)
                                                                      gcgcGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                        <  1:1486781/100‑1 (MQ=255)
                                                                         cgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                     >  1:473119/1‑100 (MQ=255)
                                                                         cgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                     >  1:1894233/1‑100 (MQ=255)
                                                                          gAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcaca                    <  2:583634/100‑1 (MQ=255)
                                                                           aaaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACaa                   <  2:1010708/100‑1 (MQ=255)
                                                                               tCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGGTCGGCGGTCGCGTCGATAATAATGGCGGACACTCCTCACGCACAAGGGc               >  2:582426/1‑100 (MQ=255)
                                                                                ccGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAggg                <  1:1029349/98‑1 (MQ=255)
                                                                                ccGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc              <  2:616658/100‑1 (MQ=255)
                                                                                 cGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc             <  2:1138312/100‑1 (MQ=255)
                                                                                   tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg           <  1:432966/100‑1 (MQ=255)
                                                                                    ggACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt          <  2:2151240/100‑1 (MQ=255)
                                                                                       cGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaaa       <  2:2319095/100‑1 (MQ=255)
                                                                                         ggCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg     <  2:463432/100‑1 (MQ=255)
                                                                                           cGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg   <  2:542444/100‑1 (MQ=255)
                                                                                           cGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg   >  2:1071392/1‑100 (MQ=255)
                                                                                           cGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg   <  1:1282135/100‑1 (MQ=255)
                                                                                            gTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg   >  1:808443/1‑99 (MQ=255)
                                                                                                |                                                                                               
GCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGT  >  NC_000913/3249532‑3249723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A