Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,249,628 G→A 54.4% G85D (GGC→GAC)  yqjD → membrane‑anchored ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,249,6280GA54.4% 29.2 / 184.8 135G85D (GGC→GAC) yqjDmembrane‑anchored ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (26/35);  new base A (26/47);  total (53/82)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 139 total aligned reads displayed.

ATCGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTG  >  NC_000913/3249529‑3249727
                                                                                                   |                                                                                                   
aTCGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTggg                                                                                                     <  2:2363654/100‑1 (MQ=255)
   gCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCAt                                                                                                  <  2:1676373/100‑1 (MQ=255)
    ccTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACAtt                                                                                                 >  1:1059676/1‑100 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgc                                                                                              >  1:81760/1‑100 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgc                                                                                              <  2:2450377/100‑1 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgc                                                                                              <  2:1286430/100‑1 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgc                                                                                              <  2:1172401/100‑1 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGCGCGCGAAAATCCGTGGACGGGCGTGGGCATTgtc                                                                                              >  1:2762615/1‑98 (MQ=255)
           gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTg                                                                                          <  1:923798/100‑1 (MQ=255)
           gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                          <  1:1760514/100‑1 (MQ=255)
            aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGc                                                                                         <  2:2878758/100‑1 (MQ=255)
             aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCa                                                                                        >  1:1832617/1‑100 (MQ=255)
             aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGc                                                                                         <  1:848504/99‑1 (MQ=255)
             aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCa                                                                                        >  1:127565/1‑100 (MQ=255)
                cGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATc                                                                                     >  2:3341661/1‑100 (MQ=255)
                 ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCg                                                                                    >  2:2572257/1‑100 (MQ=255)
                 ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATc                                                                                     <  1:1578522/99‑1 (MQ=255)
                  gTGATGCCATTGCCTAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                                   <  1:2359245/100‑1 (MQ=255)
                  gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCgg                                                                                   >  1:1933544/1‑100 (MQ=255)
                  gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                                   <  2:745101/100‑1 (MQ=255)
                     aTGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGgtgt                                                                                <  1:119135/100‑1 (MQ=255)
                     aTGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtgt                                                                                <  1:964244/100‑1 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTa                                                                               >  1:2921864/1‑100 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                               <  1:3270581/100‑1 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                               <  1:1691904/100‑1 (MQ=255)
                       gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAg                                                                              <  2:1672274/100‑1 (MQ=255)
                       gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                              <  1:581779/100‑1 (MQ=255)
                        ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGt                                                                             <  1:1868830/100‑1 (MQ=255)
                        ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGt                                                                             >  2:328824/1‑100 (MQ=255)
                        ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                             >  1:433216/1‑100 (MQ=255)
                         cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTg                                                                            >  1:2305373/1‑100 (MQ=255)
                         cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTg                                                                            >  1:1755451/1‑100 (MQ=255)
                         cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTg                                                                            <  2:2528148/100‑1 (MQ=255)
                           ttGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCt                                                                          >  1:207791/1‑100 (MQ=255)
                            tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                         >  2:3136233/1‑100 (MQ=255)
                            tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                         <  2:1218177/100‑1 (MQ=255)
                            tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGAAATCGGAGTACTGCt                                                                          >  1:2926798/1‑99 (MQ=255)
                              ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                       <  2:1675316/100‑1 (MQ=255)
                              ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                       >  2:296585/1‑100 (MQ=255)
                              ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                       <  2:1542123/100‑1 (MQ=255)
                              ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                       <  1:3221170/100‑1 (MQ=255)
                               caaacaaaCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                      >  2:1957051/1‑100 (MQ=255)
                               caaacaaaCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                      >  2:858689/1‑100 (MQ=255)
                                aaacaaacCCGTGTCGCGGCGGCGCGCGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                     >  2:2103914/1‑100 (MQ=255)
                                 aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                    <  1:2572238/100‑1 (MQ=255)
                                 aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                    >  2:3111129/1‑100 (MQ=255)
                                    aaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTct                                                                 <  1:188104/100‑1 (MQ=255)
                                     aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                                <  2:1125719/100‑1 (MQ=255)
                                      acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  2:757074/100‑1 (MQ=255)
                                      acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  2:2544685/100‑1 (MQ=255)
                                       cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  1:2528501/99‑1 (MQ=255)
                                       cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  1:3406696/99‑1 (MQ=255)
                                        ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAGATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGCTGTAGTGCTCGGCGTTctgctg                                                             <  1:2013018/100‑1 (MQ=255)
                                        ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgctg                                                             <  2:207792/100‑1 (MQ=255)
                                        ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgctg                                                             <  2:483664/100‑1 (MQ=255)
                                          gtgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTc                                                           <  1:2511844/100‑1 (MQ=255)
                                             tCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcgcg                                                        <  1:1578248/100‑1 (MQ=255)
                                              cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                       <  2:1438985/100‑1 (MQ=255)
                                                 ggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtcgt                                                    <  2:877756/100‑1 (MQ=255)
                                                    ggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTaa                                                 >  1:2631831/1‑100 (MQ=255)
                                                     gcgcGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTaa                                                 <  1:3000247/99‑1 (MQ=255)
                                                      cgcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAAtt                                               <  2:1632786/100‑1 (MQ=255)
                                                         gTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                            <  2:2261581/100‑1 (MQ=255)
                                                          tGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATgg                                           >  2:3426506/1‑100 (MQ=255)
                                                           ggcGATGGGTATGTGCGCGAAGATCCGTGGGCGGGCGTGGACATTAGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATgg                                           <  1:6078/97‑1 (MQ=255)
                                                            ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                         >  1:571458/1‑100 (MQ=255)
                                                            ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTAAGGCg                                         >  1:1135890/1‑100 (MQ=255)
                                                            ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAAGCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                         <  1:3302901/100‑1 (MQ=255)
                                                             cGATGAGTATGTGCGCGAAAATCCATGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                        <  1:1485074/100‑1 (MQ=255)
                                                              gATGAGTATGTGCGCGAACATCCCTCCACGGGCGCGGGCATTGGCGCTCCAATCGGTGTAGTGCTCGGCTTTCTGCTGTCGCCTCGTTAATTATGGCgg                                        <  1:1022194/99‑1 (MQ=255)
                                                              gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCggn                                       <  1:108/100‑2 (MQ=255)
                                                              gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGa                                       >  1:1941083/1‑100 (MQ=255)
                                                               aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                      <  2:1927416/100‑1 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   <  1:1502611/100‑1 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   <  2:1645289/100‑1 (MQ=255)
                                                                    tATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtca                                 <  1:2381634/100‑1 (MQ=255)
                                                                       gtgCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                              <  2:1879031/100‑1 (MQ=255)
                                                                         gcgcGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                            <  2:2318518/100‑1 (MQ=255)
                                                                         gcgcGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                            >  2:3167328/1‑100 (MQ=255)
                                                                          cgcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                           >  2:1342292/1‑100 (MQ=255)
                                                                           gcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGca                          >  2:324853/1‑100 (MQ=255)
                                                                            cgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                         >  2:1427590/1‑100 (MQ=255)
                                                                            cgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                         <  2:433220/100‑1 (MQ=255)
                                                                             gAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCGGTCGCTTCGATAATAATGGCGGACACTCATCACGcaca                        >  1:1686849/1‑100 (MQ=255)
                                                                              aaaaTCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACaa                       >  2:1860089/1‑100 (MQ=255)
                                                                              aaaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACaa                       <  1:2749826/100‑1 (MQ=255)
                                                                               aaaTCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAg                      <  2:1755467/100‑1 (MQ=255)
                                                                                 aTCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAggg                    <  2:497993/100‑1 (MQ=255)
                                                                                 aTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAggg                    <  1:138681/100‑1 (MQ=255)
                                                                                   ccGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc                  >  2:283659/1‑100 (MQ=255)
                                                                                   ccGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc                  >  1:1928167/1‑100 (MQ=255)
                                                                                    cGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc                 >  2:1237114/1‑100 (MQ=255)
                                                                                      tGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg               <  2:2921887/100‑1 (MQ=255)
                                                                                           gggCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTGCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaaaa          <  2:674152/100‑1 (MQ=255)
                                                                                            ggCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCCCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg         >  1:1100162/1‑100 (MQ=255)
                                                                                            ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg         <  1:1692239/100‑1 (MQ=255)
                                                                                              cGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGTCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg       <  2:2439622/100‑1 (MQ=255)
                                                                                                 gggCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTc    <  1:1440644/100‑1 (MQ=255)
                                                                                                  ggCATTGGCGCTGCAATCGGTGTAGTGCTCGGTNTTCTGCTGTTGCNTCGTTAATTATGGCGGACAATCCGCACGCCCAAGGGCCCGGTAAAAGc        >  1:2633480/1‑95 (MQ=255)
                                                                                                   gCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTg  <  2:571462/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTG  >  NC_000913/3249529‑3249727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A