Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,249,628 G→A G85D (GGC→GAC)  yqjD → membrane‑anchored ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,249,6280GA98.0% 341.3 / ‑2.1 100G85D (GGC→GAC) yqjDmembrane‑anchored ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (43/54);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

CGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTT  >  NC_000913/3249531‑3249724
                                                                                                 |                                                                                                
cGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACa                                                                                                <  1:1439853/100‑1 (MQ=255)
   cTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTg                                                                                             <  2:27074/100‑1 (MQ=255)
   cTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTg                                                                                             <  2:27207/100‑1 (MQ=255)
   cTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTg                                                                                             >  2:1804275/1‑100 (MQ=255)
    tGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTgg                                                                                            <  1:937487/100‑1 (MQ=255)
     gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgc                                                                                           <  1:2673719/100‑1 (MQ=255)
      ggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcg                                                                                          <  1:494273/100‑1 (MQ=255)
       gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcgc                                                                                         >  1:2016668/1‑100 (MQ=255)
       gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcgc                                                                                         <  1:1330119/100‑1 (MQ=255)
         gAAACCTGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                       >  2:1895487/1‑100 (MQ=255)
         gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                       >  1:339091/1‑100 (MQ=255)
         gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTg                                                                                       <  2:1011629/100‑1 (MQ=255)
           aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCa                                                                                     >  2:2485882/1‑100 (MQ=255)
           aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCa                                                                                     >  1:2710649/1‑100 (MQ=255)
             ccGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAAt                                                                                   >  1:2146180/1‑100 (MQ=255)
              cGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATc                                                                                  <  2:597864/100‑1 (MQ=255)
                gTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCgg                                                                                <  1:1615284/100‑1 (MQ=255)
                  gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                              <  1:672254/100‑1 (MQ=255)
                  gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                              <  2:523583/100‑1 (MQ=255)
                   aTGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtgt                                                                             <  1:98844/100‑1 (MQ=255)
                    tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                            <  2:24649/100‑1 (MQ=255)
                    tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                            >  2:1891913/1‑100 (MQ=255)
                     gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                           <  1:2698123/100‑1 (MQ=255)
                     gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                           <  2:2199640/100‑1 (MQ=255)
                     gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                           <  1:283621/100‑1 (MQ=255)
                     gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                           >  2:2332785/1‑100 (MQ=255)
                      ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                          <  1:1953300/100‑1 (MQ=255)
                      ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                          >  2:1040776/1‑100 (MQ=255)
                       cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTg                                                                         >  1:516897/1‑100 (MQ=255)
                          tcccAAACAAACCCGTGTCGCGGCGGCGCGTGCCTATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGTTGTAGTGCTc                                                                      <  2:1365752/98‑1 (MQ=255)
                          tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                      >  1:2091646/1‑100 (MQ=255)
                          tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                      >  2:422628/1‑100 (MQ=255)
                          tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTc                                                                      <  2:2766318/100‑1 (MQ=255)
                           gCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                     <  1:1671743/100‑1 (MQ=255)
                           gCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                     >  2:577722/1‑100 (MQ=255)
                           gCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCg                                                                     >  1:1666783/1‑100 (MQ=255)
                            ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                    <  1:2049268/100‑1 (MQ=255)
                            ccAAACAAACCCGTGTCGCGGCGGCGCGGGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                    <  1:474100/100‑1 (MQ=255)
                             caaacaaaCCCGTGTCGCGGCGGCGCGTGTCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                   >  1:134651/1‑100 (MQ=255)
                             caaacaaaCCCGCGTCGCGGCGGCGCGTGCCGATGAGCATGCGCGCGAAAAGCCGTGGACGGTCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                   <  1:905097/100‑1 (MQ=255)
                              aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                  >  2:2164447/1‑100 (MQ=255)
                              aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                  >  2:2368612/1‑100 (MQ=255)
                              aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCg                                                                  >  2:872339/1‑100 (MQ=255)
                               tcttccgatctgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                 <  1:1284452/90‑1 (MQ=255)
                               aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                 >  1:906198/1‑100 (MQ=255)
                                 caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                               <  2:1441596/100‑1 (MQ=255)
                                   aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                             >  1:1554954/1‑100 (MQ=255)
                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                            <  1:117757/100‑1 (MQ=255)
                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                            <  1:2164465/100‑1 (MQ=255)
                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                            <  2:4814/100‑1 (MQ=255)
                                    acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                            <  1:2693823/100‑1 (MQ=255)
                                     cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgct                                                           >  1:1227678/1‑100 (MQ=255)
                                            cgcgGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgt                                                    <  1:1040783/100‑1 (MQ=255)
                                               ggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtcgt                                                 <  1:633842/100‑1 (MQ=255)
                                                gcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGtt                                                >  1:448945/1‑100 (MQ=255)
                                                 cggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTa                                               <  1:1267404/100‑1 (MQ=255)
                                                        tGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATgg                                        <  1:2426400/100‑1 (MQ=255)
                                                         gCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGc                                       <  1:1752804/100‑1 (MQ=255)
                                                         gCCGATGAGGATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGc                                       <  2:1895401/100‑1 (MQ=255)
                                                          ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                      >  1:1827736/1‑100 (MQ=255)
                                                           cGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                     <  1:748318/100‑1 (MQ=255)
                                                            gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGa                                    <  2:2445417/100‑1 (MQ=255)
                                                            gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGa                                    <  2:1287186/100‑1 (MQ=255)
                                                              tGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATTGCg                                      >  1:2476948/1‑96 (MQ=255)
                                                              tGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                   <  1:35312/99‑1 (MQ=255)
                                                              tGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                   <  2:1735417/99‑1 (MQ=255)
                                                              tGAGTATGTGCGCGAAAATCCGTAGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGGTTGGCGTTCTGCTGTCGAGTCGTTAATTATGGCgg                                     >  2:2713962/1‑97 (MQ=255)
                                                                aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                >  1:2730728/1‑100 (MQ=255)
                                                                aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                <  2:516893/100‑1 (MQ=255)
                                                                    tgtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatc                            <  2:1971364/100‑1 (MQ=255)
                                                                    tgtgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatc                            <  1:1559117/100‑1 (MQ=255)
                                                                     gtgCGCGAAAATCCGTGGACGGGCGTGGCCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCGGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                           <  1:1895502/100‑1 (MQ=255)
                                                                      tgCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCAc                          >  2:325884/1‑100 (MQ=255)
                                                                       gcgcGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                         >  1:2728819/1‑100 (MQ=255)
                                                                       gcgcGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                         >  2:2413410/1‑100 (MQ=255)
                                                                        cgcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                        >  2:765542/1‑100 (MQ=255)
                                                                        cgcgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                        >  1:1371468/1‑100 (MQ=255)
                                                                          cgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                      >  1:1441245/1‑100 (MQ=255)
                                                                             aaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAg                   <  2:585131/100‑1 (MQ=255)
                                                                             aaaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAg                   <  1:1328208/100‑1 (MQ=255)
                                                                              aaTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAgg                  <  1:641412/100‑1 (MQ=255)
                                                                               aTCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAggg                 <  2:2610191/100‑1 (MQ=255)
                                                                                 ccGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc               <  2:74754/100‑1 (MQ=255)
                                                                                 ccGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc               <  2:1832295/100‑1 (MQ=255)
                                                                                  cGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc              >  1:1129605/1‑100 (MQ=255)
                                                                                  cGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc              <  2:1022218/100‑1 (MQ=255)
                                                                                  cGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc              >  1:619720/1‑100 (MQ=255)
                                                                                  cGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGccc              >  1:1427431/1‑100 (MQ=255)
                                                                                  cGTGGACGGGCGTGGACATTGGCGATGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGcc               >  2:1702106/1‑99 (MQ=255)
                                                                                    tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTACTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg            <  1:1938323/100‑1 (MQ=255)
                                                                                     ggACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt           <  1:765547/100‑1 (MQ=255)
                                                                                       aCGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaa         >  1:2350520/1‑100 (MQ=255)
                                                                                        cGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGAGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTaaa        >  2:619491/1‑100 (MQ=255)
                                                                                          ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg      <  2:1666770/100‑1 (MQ=255)
                                                                                          ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg      >  2:927554/1‑100 (MQ=255)
                                                                                            cGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg    >  2:2530940/1‑100 (MQ=255)
                                                                                            cGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCg    >  2:1481578/1‑100 (MQ=255)
                                                                                              tGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGtt  >  2:1415292/1‑100 (MQ=255)
                                                                                              tGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGtt  >  1:1456837/1‑100 (MQ=255)
                                                                                              tGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGtt  >  1:1456812/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTT  >  NC_000913/3249531‑3249724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A