Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,249,628 G→A 25.1% G85D (GGC→GAC)  yqjD → membrane‑anchored ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,249,6280GA25.1% 313.0 / 98.7 170G85D (GGC→GAC) yqjDmembrane‑anchored ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (58/69);  new base A (22/21);  total (80/90)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

ATCGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTG  >  NC_000913/3249529‑3249727
                                                                                                   |                                                                                                   
atggCCTGGGTGAAGCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTggg                                                                                                     <  1:39863/97‑1 (MQ=255)
      ttggTGAGACCGGTGATGCCATTGCCAAGCCAGCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAGTCCGTGGACGGGCGTGGGCATTgg                                                                                               <  1:2746294/98‑1 (MQ=255)
      tGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTgg                                                                                               >  2:3049703/1‑100 (MQ=255)
       gggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTggt                                                                                              <  2:1870416/100‑2 (MQ=255)
        ggTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgcg                                                                                             >  1:457584/1‑100 (MQ=255)
         gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgcgc                                                                                            <  2:2740349/100‑1 (MQ=255)
         gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgcgc                                                                                            <  2:107665/100‑1 (MQ=255)
         gTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGgcgc                                                                                            <  1:1970222/100‑1 (MQ=255)
          tGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGgcg                                                                                             >  1:66937/1‑98 (MQ=255)
          tGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCt                                                                                           >  2:2797922/1‑100 (MQ=255)
           gAAAGCGGTGATGCCACTGCCAACCGAACCCGTGTCGCGGCGGCGCGGGCCGATGAGGATGTGCGCGAAAATCCGTGGACGGGCGGGGGCATTGGCGCTg                                                                                          <  1:3541264/100‑1 (MQ=255)
           gAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTg                                                                                          >  1:1494065/1‑100 (MQ=255)
            aaaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGc                                                                                         <  2:433782/100‑1 (MQ=255)
             aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCa                                                                                        >  2:2727863/1‑100 (MQ=255)
             aaCCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCa                                                                                        >  2:1486099/1‑100 (MQ=255)
               ccGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGGATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAAt                                                                                      >  2:1020078/1‑100 (MQ=255)
                 ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCaa                                                                                       <  1:2525608/97‑1 (MQ=255)
                 ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCg                                                                                    >  2:1109815/1‑100 (MQ=255)
                 ggTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCg                                                                                    <  1:426548/100‑1 (MQ=255)
                    gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGgtg                                                                                 <  2:588779/100‑1 (MQ=255)
                    gATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGgtg                                                                                 <  2:469629/100‑1 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTa                                                                               <  1:4035216/100‑1 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGgtg                                                                                 <  2:1456426/98‑1 (MQ=255)
                      tGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTa                                                                               >  2:1180652/1‑100 (MQ=255)
                       gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAg                                                                              <  2:2328582/100‑1 (MQ=255)
                       gCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGGGGGCATTGGCGCTGCAATCGGTGTa                                                                               >  1:3808606/1‑99 (MQ=255)
                        ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGt                                                                             >  1:3455742/1‑100 (MQ=255)
                        ccATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGt                                                                             >  1:999243/1‑100 (MQ=255)
                         cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTg                                                                            >  2:4035816/1‑100 (MQ=255)
                         cATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTg                                                                            >  2:2280569/1‑100 (MQ=255)
                           ttGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCt                                                                          >  2:3844970/1‑100 (MQ=255)
                            tGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGTATTGGCGCTGCAATCGGTGTAGTGCTc                                                                         <  1:1870978/100‑1 (MQ=255)
                              ccAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCgg                                                                       >  2:1550244/1‑100 (MQ=255)
                               caaacaaaCCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGc                                                                      >  1:508174/1‑100 (MQ=255)
                                aaacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGTTGCAATCGGTGTAGTGCTCGGCg                                                                     >  2:2567117/1‑100 (MQ=255)
                                 aacgaccccGTTTCGCGGCGGCGCGTGCCGCTGAGTATGTGCGCGAAAATCAGTGGAGGGGGGTGGACATTGGCGTTGAAGTTGGTGTGGTGTTAGGc                                                                      >  1:1639684/7‑98 (MQ=255)
                                 aacaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGt                                                                    <  2:2553420/100‑1 (MQ=255)
                                  acaaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGtt                                                                   >  2:178776/1‑100 (MQ=255)
                                   caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                                  <  1:1109812/100‑1 (MQ=255)
                                   caaacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTc                                                                  <  1:1658114/100‑1 (MQ=255)
                                     aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                                >  1:4216362/1‑100 (MQ=255)
                                     aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                                >  2:1679685/1‑100 (MQ=255)
                                     aacCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGGTc                                                                  >  1:2595088/1‑98 (MQ=255)
                                     aacCCGTGTCGAGGCGGCGCGTGCCGATGAGTATGTGCTAGAAAATGCGTGGGCGGGCGCGGACATCGGCGCTTCAATCGGTGGAGTg                                                                            >  1:142029/1‑88 (MQ=255)
                                      acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  2:3346049/100‑1 (MQ=255)
                                      acCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgc                                                               <  2:2206265/100‑1 (MQ=255)
                                       cccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgct                                                              >  2:2468046/1‑100 (MQ=255)
                                        ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgctg                                                             <  2:2621252/100‑1 (MQ=255)
                                        ccGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctgctg                                                             >  1:856459/1‑100 (MQ=255)
                                         cGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTctg                                                                <  1:3620043/96‑1 (MQ=255)
                                           tgtCGCGGCGGCGCGTGCCGATGATTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                          <  2:4150996/100‑1 (MQ=255)
                                           tgtCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTcg                                                          <  2:3562379/100‑1 (MQ=255)
                                               gcggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtc                                                      <  1:1633444/100‑1 (MQ=255)
                                                cggcggcgCGTGTCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtcg                                                     <  2:3214635/100‑1 (MQ=255)
                                                cggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGGGTGGACATTGGCGCGGCAAACCGGGTAGTGCTCGGCGTTCTGCCGt                                                            >  1:3918185/1‑93 (MQ=255)
                                                 ggcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGcgtcgt                                                    >  1:1559910/1‑100 (MQ=255)
                                                  gcggcgCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGtt                                                   <  2:2017880/100‑1 (MQ=255)
                                                       gcgTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTa                                              <  1:539014/100‑1 (MQ=255)
                                                         gTGCCGATGAGTATGTGCGCGAAAATCCGTGTACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                            <  1:175472/100‑1 (MQ=255)
                                                         gTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATg                                            >  1:2301546/1‑100 (MQ=255)
                                                           gCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGc                                          <  1:4100453/100‑1 (MQ=255)
                                                            ccGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCg                                         <  2:804513/100‑1 (MQ=255)
                                                             cGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCgg                                        <  1:785385/100‑1 (MQ=255)
                                                              gATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGa                                       <  2:711402/100‑1 (MQ=255)
                                                               aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                      <  1:1038400/100‑1 (MQ=255)
                                                               aTGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                      <  2:1903747/100‑1 (MQ=255)
                                                                 gcgTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGacac                                    <  2:3082545/98‑1 (MQ=255)
                                                                 gAGCATGCGCGCGAAAATCCATGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGac                                      <  1:1299804/98‑1 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   >  2:544470/1‑100 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   >  1:3107315/1‑100 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   >  2:251519/1‑100 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   >  1:1155487/1‑100 (MQ=255)
                                                                  aGTATGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACt                                   <  2:3429696/100‑1 (MQ=255)
                                                                     aTGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcat                                >  1:295902/1‑100 (MQ=255)
                                                                     aTGTGCGCGAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcat                                <  2:1669234/100‑1 (MQ=255)
                                                                       gtgCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCATTCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                              <  1:3101242/100‑1 (MQ=255)
                                                                       gtgCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACtcatca                              >  2:4032489/1‑100 (MQ=255)
                                                                         gcgcGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACg                            >  2:952398/1‑100 (MQ=255)
                                                                          cgcgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                           >  1:3787398/1‑100 (MQ=255)
                                                                          cgcgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGc                           <  1:1486093/100‑1 (MQ=255)
                                                                           gcgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGCTCTGCTGTCGCGTCGTTAATTATGGCGGTCACTCATCACGca                          <  2:2986934/100‑1 (MQ=255)
                                                                            cgAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                         <  1:1664887/100‑1 (MQ=255)
                                                                            cgAAAATCCGTGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGcac                         >  2:2397006/1‑100 (MQ=255)
                                                                                aaTCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGATCTCGGTGGTCTGCGGACGCGTAATTAATTATGGCGGACACTCATCACGCACAAg                      >  1:684820/1‑99 (MQ=255)
                                                                                    cGTGGACGGGGGTGCGGGGGGGCGCTGCAATCGGTGGAGCGCGCGGCGCTGTGCAGTCGAGTCGTTAATTATGGGGGGCACTAGTCACGCATAAggg                    <  1:2614640/97‑1 (MQ=255)
                                                                                      tGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg               <  2:4137354/100‑1 (MQ=255)
                                                                                      tGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg               <  2:4373361/100‑1 (MQ=255)
                                                                                      tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg               <  1:2032431/100‑1 (MQ=255)
                                                                                      tGGACGGGCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCgg               <  2:478590/100‑1 (MQ=255)
                                                                                       ggACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt              >  1:2182613/1‑100 (MQ=255)
                                                                                       ggACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGt              <  2:16968/100‑1 (MQ=255)
                                                                                            ggCGTGGGCATTGGCGCTGCAATCGGTGTAGTTCTCGGTGGTCGGCGGATCATTCATAAATTATGGCGGACACTCATCACGCACAAAGGCCCGGAAAAAg         >  1:2409647/1‑100 (MQ=255)
                                                                                            ggCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAg         >  2:3270969/1‑100 (MQ=255)
                                                                                             gCGTGGACATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGc        <  2:1321825/100‑1 (MQ=255)
                                                                                                tGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGtt     <  1:2035397/100‑1 (MQ=255)
                                                                                                tGGGCATTGACGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGtt     >  1:2834169/1‑100 (MQ=255)
                                                                                                  ggCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCt   >  1:464779/1‑100 (MQ=255)
                                                                                                  ggCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCt   >  2:1896100/1‑100 (MQ=255)
                                                                                                   gCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTg  >  2:4261042/1‑100 (MQ=255)
                                                                                                   gCATTGACGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTg  <  2:2834186/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATCGCCTGGGTGAAACCGGTGATGCCATTGCCAAACAAACCCGTGTCGCGGCGGCGCGTGCCGATGAGTATGTGCGCGAAAATCCGTGGACGGGCGTGGGCATTGGCGCTGCAATCGGTGTAGTGCTCGGCGTTCTGCTGTCGCGTCGTTAATTATGGCGGACACTCATCACGCACAAGGGCCCGGTAAAAGCGTTCTG  >  NC_000913/3249529‑3249727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A