Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F200 I0 R1 101 105.4 5209919 94.4% 4918163 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,952,569 C→T 15.8% A29V (GCG→GTG)  ilvE → branched‑chain amino acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,5690CT15.8% 296.1 / 33.2 118A29V (GCG→GTG) ilvEbranched‑chain amino acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (43/56);  new base T (9/10);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 128 total aligned reads displayed.

AAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA  >  NC_000913/3952470‑3952668
                                                                                                   |                                                                                                   
aaaGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCAcgc                                                                                                     >  1:1811773/1‑100 (MQ=255)
aaaGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCAcgc                                                                                                     >  2:2300613/1‑100 (MQ=255)
 aaGGAATATAAAAATGACCACGAAGGAAGCTGATTAAATTTGGTGCAATGGGGAGATGGTCCGCTGGGAAGATACGAAGATGCATGTGATGTCGCAcgcg                                                                                                    >  2:16106/1‑100 (MQ=255)
   ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAAATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCAcgcg                                                                                                    >  2:2303163/1‑98 (MQ=255)
     aaTATAAAAATGACTACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                                <  2:1770975/100‑1 (MQ=255)
     aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGc                                                                                                <  1:400723/100‑1 (MQ=255)
      atatAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCa                                                                                               <  2:2599672/100‑1 (MQ=255)
      atatAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCa                                                                                               <  2:165375/100‑1 (MQ=255)
        ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACt                                                                                             >  2:591979/1‑100 (MQ=255)
        ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGATGCATGTGATATCGCACGCGCTgaaat                                                                                             >  2:1283217/1‑96 (MQ=255)
         taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTa                                                                                            >  1:394020/1‑100 (MQ=255)
         taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTa                                                                                            >  2:1726713/1‑100 (MQ=255)
         taAAAATGACCACGAAGAAAGCTGATTACAGTTGGTTCAATGGGGAGAAGGTTCGCTGGGCAGACGCGAAGGTGCATGTGATGTCGCACGCGATGCAcaa                                                                                            >  2:550583/1‑98 (MQ=255)
            aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATgg                                                                                         >  2:1110285/1‑100 (MQ=255)
             aaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGc                                                                                        <  2:919185/100‑1 (MQ=255)
             aaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGc                                                                                        <  1:1355939/100‑1 (MQ=255)
              aTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGc                                                                                        <  1:146154/99‑1 (MQ=255)
                  ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACtcc                                                                                   >  1:1342020/1‑98 (MQ=255)
                   cACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCg                                                                                  >  1:2075336/1‑100 (MQ=255)
                    aCGGAGATAGCTGATTACATTTGGTTCAATGGGGGGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCgg                                                                                 <  2:1762767/100‑1 (MQ=255)
                    aCGAAGAAAGCTGATTACATTTGGTTCAATGTGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCg                                                                                  <  2:742924/99‑1 (MQ=255)
                    aCGAAGAAAGCTGATTACATTTGGGTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCgg                                                                                 >  1:517655/1‑100 (MQ=255)
                     cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGt                                                                                >  2:584531/1‑100 (MQ=255)
                     cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGt                                                                                <  1:2117183/100‑1 (MQ=255)
                            aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGaa                                                                         <  1:2028717/100‑1 (MQ=255)
                            aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGaa                                                                         <  2:1485185/100‑1 (MQ=255)
                            aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGaa                                                                         <  2:469224/100‑1 (MQ=255)
                            aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGaa                                                                         <  2:1484826/100‑1 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                        <  2:1867846/100‑1 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                        <  1:591982/100‑1 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGCCACTTCGGTTTTTGAAg                                                                        >  2:482999/1‑100 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                        <  1:694242/100‑1 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                        >  2:460023/1‑100 (MQ=255)
                             gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                        <  1:291805/100‑1 (MQ=255)
                              cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                       <  1:442925/100‑1 (MQ=255)
                               tGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGc                                                                      >  1:1291951/1‑100 (MQ=255)
                                gATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCa                                                                     >  2:2021495/1‑100 (MQ=255)
                                gATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCa                                                                     >  1:1599844/1‑100 (MQ=255)
                                  ttACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATc                                                                   >  2:1438154/1‑100 (MQ=255)
                                     cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                >  1:392080/1‑100 (MQ=255)
                                       tttGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                >  2:2164033/1‑98 (MQ=255)
                                       tttGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTg                                                              <  2:616119/100‑1 (MQ=255)
                                        ttGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGc                                                             >  1:690524/1‑100 (MQ=255)
                                         tGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCt                                                            <  1:1611085/100‑1 (MQ=255)
                                          ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTa                                                           <  1:1406393/100‑1 (MQ=255)
                                             tCAATGGGGAGATGGTTCGCGGGGAAGACGCGAAGATGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGa                                                        >  2:1593462/1‑100 (MQ=255)
                                              cAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGAc                                                       <  2:1687574/100‑1 (MQ=255)
                                                aTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                     <  1:351321/100‑1 (MQ=255)
                                                aTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                     <  2:2191166/100‑1 (MQ=255)
                                                 tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCGGTTGCTACGACTCg                                                    >  2:1772985/1‑100 (MQ=255)
                                                  ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   <  2:297133/100‑1 (MQ=255)
                                                  ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   >  2:938131/1‑100 (MQ=255)
                                                  ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   <  2:1732198/100‑1 (MQ=255)
                                                   gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGca                                                  <  1:1152453/100‑1 (MQ=255)
                                                    ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                                 >  2:1582233/1‑100 (MQ=255)
                                                       gaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATTTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACaaa                                              >  1:2030397/1‑100 (MQ=255)
                                                       gaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACaaa                                              >  1:2493381/1‑100 (MQ=255)
                                                        aTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAg                                             <  1:2603734/100‑1 (MQ=255)
                                                        aTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAg                                             <  1:2012640/100‑1 (MQ=255)
                                                         tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAgg                                            <  1:105445/100‑1 (MQ=255)
                                                            ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                         <  2:81552/100‑1 (MQ=255)
                                                            ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                         <  1:2314079/100‑1 (MQ=255)
                                                               gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                      >  1:241690/1‑100 (MQ=255)
                                                                cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                     >  2:978873/1‑100 (MQ=255)
                                                                cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                     <  1:2228788/100‑1 (MQ=255)
                                                                 tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                                    <  2:1038576/100‑1 (MQ=255)
                                                                  gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                                   >  1:1242627/1‑100 (MQ=255)
                                                                  gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                                   >  1:1115571/1‑100 (MQ=255)
                                                                  gggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                                   <  2:1796890/100‑1 (MQ=255)
                                                                    gAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGATGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                 <  2:76786/100‑1 (MQ=255)
                                                                    gAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                 >  1:2420896/1‑100 (MQ=255)
                                                                        aCGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCg                             <  2:27087/100‑1 (MQ=255)
                                                                         cgcgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            <  2:8333/100‑1 (MQ=255)
                                                                         cgcgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTCGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            >  2:2304487/1‑100 (MQ=255)
                                                                           cgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                          >  1:843133/1‑100 (MQ=255)
                                                                               ggTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGt                      <  1:1264583/100‑1 (MQ=255)
                                                                                gTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTg                     <  1:584534/100‑1 (MQ=255)
                                                                                 tGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGa                    >  1:2142248/1‑100 (MQ=255)
                                                                                  gCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAg                   <  1:1391730/100‑1 (MQ=255)
                                                                                  gCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAg                   <  1:1008983/100‑1 (MQ=255)
                                                                                   cATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGc                  <  2:2194918/100‑1 (MQ=255)
                                                                                   cATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGc                  <  1:1902517/100‑1 (MQ=255)
                                                                                      gtgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata               <  1:1614349/100‑1 (MQ=255)
                                                                                       tgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGGAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat              >  1:1512283/1‑100 (MQ=255)
                                                                                       tgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat              >  1:89536/1‑100 (MQ=255)
                                                                                          tGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCa           >  2:1893321/1‑100 (MQ=255)
                                                                                          tGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCa           >  1:2219365/1‑100 (MQ=255)
                                                                                           gTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg          <  1:758431/100‑1 (MQ=255)
                                                                                            tCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGc         <  2:1219869/100‑1 (MQ=255)
                                                                                             cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        >  1:1876128/1‑100 (MQ=255)
                                                                                             cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        >  1:1909521/1‑100 (MQ=255)
                                                                                             cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        >  1:847223/1‑100 (MQ=255)
                                                                                             cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        <  1:938136/100‑1 (MQ=255)
                                                                                             cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        >  1:1434757/1‑100 (MQ=255)
                                                                                               cACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCAGCCGTCGCGTCGCCTCGCACAAAGGGCCGGGGGTATACCGCCATCCTGAGCATATGCAGCGt       <  2:233705/99‑1 (MQ=255)
                                                                                                aCGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCt     <  2:2260779/100‑1 (MQ=255)
                                                                                                 cgcgCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTg    <  1:2113047/100‑1 (MQ=255)
                                                                                                   cgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa  <  1:1034918/100‑1 (MQ=255)
                                                                                                   cgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa  >  1:2304643/1‑100 (MQ=255)
                                                                                                   cgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa  >  1:324297/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
AAAGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA  >  NC_000913/3952470‑3952668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A