Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,952,569 C→T 100% A29V (GCG→GTG)  ilvE → branched‑chain amino acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,5690CT95.9% 291.8 / 2.2 97A29V (GCG→GTG) ilvEbranched‑chain amino acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (41/52);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

GGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCG  >  NC_000913/3952473‑3952662
                                                                                                |                                                                                             
ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                            >  1:1068063/1‑100 (MQ=255)
  aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                          <  2:2101922/100‑1 (MQ=255)
  aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                          <  1:1983828/100‑1 (MQ=255)
   atatAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCa                                                                                         >  2:888293/1‑100 (MQ=255)
     ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGGACGTGCTGCAc                                                                                        >  1:2378833/1‑99 (MQ=255)
     ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACt                                                                                       >  2:226692/1‑100 (MQ=255)
     ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACt                                                                                       >  2:2186911/1‑100 (MQ=255)
     ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACt                                                                                       <  1:959807/100‑1 (MQ=255)
      taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGCAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCAc                                                                                        >  1:926332/1‑98 (MQ=255)
      taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                      >  2:538878/1‑100 (MQ=255)
         aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATgg                                                                                   <  2:711563/100‑1 (MQ=255)
          gatctccACGAAGAAAGCTGATTACATTTGGTTCTATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCTCTATGGc                                                                                  <  1:1358139/95‑1 (MQ=255)
             gACCACGAAGAAAGCTGCGTACATTGGGTTCAATGGGGAGATGGCTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTCTGGc                                                                                  <  1:1454338/97‑1 (MQ=255)
               ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTc                                                                             >  1:824459/1‑100 (MQ=255)
               ccACGAACAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCagatc                                                                             >  2:1358147/1‑96 (MQ=255)
                cACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCg                                                                            <  2:599954/100‑1 (MQ=255)
                  cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGt                                                                          >  2:590075/1‑100 (MQ=255)
                  cGAAGAAAGCTGATTACATTAGGTTCAATGGGGAGATGGTTCGCTAGGAAGACGCGATGGTGCATGTGATGTCGCATGTGCTGCACTATGGCTCTTCGGt                                                                          >  1:2441681/1‑100 (MQ=255)
                      gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                      <  1:1316181/100‑1 (MQ=255)
                      gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                      >  1:2226053/1‑100 (MQ=255)
                       aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                     <  1:29249/100‑1 (MQ=255)
                       aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                     <  2:2019742/100‑1 (MQ=255)
                        aaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGa                                                                    >  2:118975/1‑100 (MQ=255)
                          ncTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                  >  2:1411534/2‑100 (MQ=255)
                          gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                  <  2:538978/100‑1 (MQ=255)
                          gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                  <  2:690486/100‑1 (MQ=255)
                           cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                 <  2:2179720/100‑1 (MQ=255)
                           cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                 <  1:609223/100‑1 (MQ=255)
                           cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                 <  1:1078086/100‑1 (MQ=255)
                           cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                 <  1:2212347/100‑1 (MQ=255)
                            tGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGc                                                                <  2:2138474/100‑1 (MQ=255)
                              aTTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCa                                                               <  2:2277208/99‑1 (MQ=255)
                               ttACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATc                                                             >  2:2279423/1‑100 (MQ=255)
                               tgaCATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATc                                                             >  2:1757070/3‑100 (MQ=255)
                                    tttGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTg                                                        <  1:1494572/100‑1 (MQ=255)
                                       ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTa                                                     >  1:706621/1‑100 (MQ=255)
                                        gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                    >  2:2151809/1‑100 (MQ=255)
                                         ttCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACg                                                   <  2:1032088/100‑1 (MQ=255)
                                          tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGa                                                  >  2:1898781/1‑100 (MQ=255)
                                          tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGa                                                  >  1:2062301/1‑100 (MQ=255)
                                            aaTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACt                                                >  1:1691405/1‑100 (MQ=255)
                                             aTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                               >  1:2033294/1‑100 (MQ=255)
                                                gggAGATGGTTCGCTGGGAAGACGCGAAGGTGTATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                             <  1:856316/99‑1 (MQ=255)
                                                gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGca                                            <  1:2533595/100‑1 (MQ=255)
                                                 ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                           <  2:644303/100‑1 (MQ=255)
                                                 ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                           >  2:211948/1‑100 (MQ=255)
                                                 ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                           >  1:778772/1‑100 (MQ=255)
                                                  gagaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcaca                                          <  1:2515511/100‑1 (MQ=255)
                                                   agaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACaa                                         <  1:2268295/100‑1 (MQ=255)
                                                     aTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAg                                       <  2:1717902/100‑1 (MQ=255)
                                                     aTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAg                                       <  1:1325962/100‑1 (MQ=255)
                                                      tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAgg                                      <  2:2338008/100‑1 (MQ=255)
                                                        gTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAc                                    >  1:760831/1‑100 (MQ=255)
                                                        gTTCGCTGGGAAGAAGCGAAGGTGAATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAc                                    <  1:492258/100‑1 (MQ=255)
                                                         ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                   <  2:2033306/100‑1 (MQ=255)
                                                         ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                   <  2:446168/100‑1 (MQ=255)
                                                         ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                   >  2:2010520/1‑100 (MQ=255)
                                                          tCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCg                                  >  1:1447296/1‑100 (MQ=255)
                                                           cGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCgg                                 >  2:2024968/1‑100 (MQ=255)
                                                            gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                >  2:772566/1‑100 (MQ=255)
                                                            gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                <  2:784719/100‑1 (MQ=255)
                                                            gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                >  2:1132242/1‑100 (MQ=255)
                                                              tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                              <  1:690502/100‑1 (MQ=255)
                                                              tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                              <  2:1332552/100‑1 (MQ=255)
                                                               gggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                             <  1:643208/100‑1 (MQ=255)
                                                               gggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                             <  1:1948049/100‑1 (MQ=255)
                                                                ggAAGACGCGAAGGTGCATGGGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTa                            <  1:594976/100‑1 (MQ=255)
                                                                 gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                           >  2:2392143/1‑100 (MQ=255)
                                                                  aaGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                          >  1:40728/1‑100 (MQ=255)
                                                                     aCGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCg                       <  1:1898771/100‑1 (MQ=255)
                                                                      ctcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                      >  2:1759570/3‑100 (MQ=255)
                                                                      cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                      <  1:984242/100‑1 (MQ=255)
                                                                      cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                      <  2:2263960/100‑1 (MQ=255)
                                                                      cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                      <  2:104018/100‑1 (MQ=255)
                                                                      cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGATTGTATTCCGc                      >  1:1665619/1‑100 (MQ=255)
                                                                        cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                    >  1:818492/1‑100 (MQ=255)
                                                                        cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATCCCGCCa                    >  1:2059532/1‑100 (MQ=255)
                                                                        cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGGATTCCGCCa                    >  1:1572346/1‑100 (MQ=255)
                                                                          aaGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATc                  <  2:1691230/100‑1 (MQ=255)
                                                                           aGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAATGGACCGGTTGTATTCCGCCATCg                 <  1:984636/100‑1 (MQ=255)
                                                                            ggTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGt                <  2:2101043/100‑1 (MQ=255)
                                                                            ggTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGt                <  2:503934/100‑1 (MQ=255)
                                                                                 aTGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCa           <  1:863605/100‑1 (MQ=255)
                                                                                 aTGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCa           <  2:511711/100‑1 (MQ=255)
                                                                                 aTGTGATGGCGCATGTGCTGCACTATGGAACTTCGGTTTGTAAAGTTATCCGTTGCTAACGCTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGc            <  1:805125/99‑1 (MQ=255)
                                                                                  tgtgATGTCGCACGTGCTGCACTATGGCACTTCGGTCTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat          <  2:472316/100‑1 (MQ=255)
                                                                                   gtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata         <  2:2393874/100‑1 (MQ=255)
                                                                                   gtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata         <  1:717948/100‑1 (MQ=255)
                                                                                    ttaTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat        >  2:1742859/3‑100 (MQ=255)
                                                                                      aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc      <  2:2082146/100‑1 (MQ=255)
                                                                                      aTGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatattc      >  1:1569560/1‑98 (MQ=255)
                                                                                        gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg    <  1:728456/100‑1 (MQ=255)
                                                                                        gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg    >  1:2529219/1‑100 (MQ=255)
                                                                                         tCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc      >  1:994369/1‑97 (MQ=255)
                                                                                          cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg  >  2:2360319/1‑100 (MQ=255)
                                                                                          cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg  <  1:2147016/100‑1 (MQ=255)
                                                                                          cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg  >  2:792197/1‑100 (MQ=255)
                                                                                          cGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg  <  1:2406027/100‑1 (MQ=255)
                                                                                            cACGTGCTGCACTATGGCACATCGGTTTTTGAAGGTATCCGTTGCTACGAGTCGCCCAAAGGACCGGTTGTATTCCGACATCGTGAGCATATGCAGCg  <  1:1267033/98‑1 (MQ=255)
                                                                                                |                                                                                             
GGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCG  >  NC_000913/3952473‑3952662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A