Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F200 I1 R1 100 75.0 3643689 96.7% 3523447 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,952,569 C→T A29V (GCG→GTG)  ilvE → branched‑chain amino acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,5690CT100.0% 211.8 / NA 62A29V (GCG→GTG) ilvEbranched‑chain amino acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/29);  total (33/29)

AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT  >  NC_000913/3952472‑3952663
                                                                                                 |                                                                                              
aGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACgtgc                                                                                              <  1:643621/100‑1 (MQ=255)
 ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                             >  2:551517/1‑100 (MQ=255)
 ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                             >  2:1212911/1‑100 (MQ=255)
  gAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGGTGGGAAGAAGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                             >  1:819213/1‑99 (MQ=255)
  gAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTg                                                                                            >  2:204497/1‑100 (MQ=255)
   aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                           >  1:923888/1‑100 (MQ=255)
    atatAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCa                                                                                          <  1:204497/100‑1 (MQ=255)
     tataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCAc                                                                                         <  1:1508278/100‑1 (MQ=255)
      ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACt                                                                                        >  2:1142612/1‑100 (MQ=255)
       tgaaaaTGACCACGAAGAAAGCTGATTACATTTGGTTCGATGGCGAGATGGTTCGCTGGGAAGCAGCGACGGTGAATGTGATGTCGCACGTGCTGCAcca                                                                                       >  1:985300/3‑98 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                       <  1:17319/100‑1 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                       >  2:383615/1‑100 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGAGAAGACGCGAACGTGCATGTGATGTCGCATGTGCTGCACt                                                                                        >  1:1234629/1‑99 (MQ=255)
         aaaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATg                                                                                     >  1:933509/1‑100 (MQ=255)
         aaaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATg                                                                                     <  1:739146/100‑1 (MQ=255)
           aaTGACCACGAAGAAAGCTGATTAGATGTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGc                                                                                   <  1:19392/100‑1 (MQ=255)
           aaTGACCACGAAGAAAGCTGATTACATTTTGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGc                                                                                   <  1:359589/100‑1 (MQ=255)
                ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTc                                                                              >  2:708704/1‑100 (MQ=255)
                 cACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCg                                                                             <  2:785764/100‑1 (MQ=255)
                   cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGCTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGt                                                                           <  1:989725/100‑1 (MQ=255)
                    gaagaaCGCTGATTATATTTGGTTCAATGGTGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGtt                                                                          <  2:1633083/100‑1 (MQ=255)
                     aagaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttt                                                                         <  2:1133953/100‑1 (MQ=255)
                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                       >  1:763405/1‑100 (MQ=255)
                         aaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                       <  1:1708362/98‑1 (MQ=255)
                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                  <  2:449971/100‑1 (MQ=255)
                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                   <  2:1654998/99‑1 (MQ=255)
                                  aCATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCg                                                            <  2:742670/100‑1 (MQ=255)
                                   cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                           >  1:131445/1‑100 (MQ=255)
                                        ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTa                                                      >  2:223536/1‑100 (MQ=255)
                                        ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTa                                                      >  1:533139/1‑100 (MQ=255)
                                         gTTTAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                     <  1:1281299/100‑1 (MQ=255)
                                         gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                     >  1:161331/1‑100 (MQ=255)
                                           tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGa                                                   <  2:104052/100‑1 (MQ=255)
                                            cAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGTCACTTCGGTTTTTGAAGGCATCCGTTGCTACGAc                                                  >  1:362151/1‑100 (MQ=255)
                                               tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCg                                               >  1:1400642/1‑100 (MQ=255)
                                               tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCg                                               >  2:497614/1‑100 (MQ=255)
                                               tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAAGCATCCGTTGCTACGACTCg                                               >  2:1601705/1‑100 (MQ=255)
                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                >  1:1646204/1‑98 (MQ=255)
                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                              >  1:225415/1‑100 (MQ=255)
                                                       tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGATTTTGAAAGCATCCGTTGCTACGACTCGCACAAAgg                                       >  2:684751/1‑100 (MQ=255)
                                                          ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                    <  1:24663/100‑1 (MQ=255)
                                                            cGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCgg                                  >  1:960947/1‑100 (MQ=255)
                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGTACCGGt                                 >  1:1067326/1‑100 (MQ=255)
                                                               tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                               <  2:923894/100‑1 (MQ=255)
                                                               tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                               <  2:39267/100‑1 (MQ=255)
                                                                gggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                              <  1:1454069/100‑1 (MQ=255)
                                                                 ggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTACACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTa                             >  1:1387423/1‑100 (MQ=255)
                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                            >  2:1696716/1‑100 (MQ=255)
                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                            <  1:1767682/100‑1 (MQ=255)
                                                                    aGACGCGAAGGGGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGTTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAttn                          <  1:848043/100‑2 (MQ=255)
                                                                      aCGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCg                        <  1:380038/100‑1 (MQ=255)
                                                                       cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTcc                         >  2:1243473/1‑98 (MQ=255)
                                                                            aGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCg                  <  2:1101130/100‑1 (MQ=255)
                                                                                  aTGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCa            <  2:619859/100‑1 (MQ=255)
                                                                                      gATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATg        >  1:202868/1‑100 (MQ=255)
                                                                                      gATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCAATCGTGAGAATATg        >  2:230918/1‑100 (MQ=255)
                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  2:1098311/100‑1 (MQ=255)
                                                                                         gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg     >  2:992117/1‑100 (MQ=255)
                                                                                         gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg     <  2:487314/100‑1 (MQ=255)
                                                                                          tCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGc    <  1:551513/100‑1 (MQ=255)
                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg   <  2:1066266/100‑1 (MQ=255)
                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg   >  2:796672/1‑100 (MQ=255)
                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg   >  1:1066256/1‑100 (MQ=255)
                                                                                            gCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt  >  1:976689/1‑100 (MQ=255)
                                                                                                 |                                                                                              
AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT  >  NC_000913/3952472‑3952663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A