Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F244 I1 R1	118	120.6	6073099	93.5%	5678347	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,952,569	C→T	A29V (GCG→GTG)	ilvE →	branched‑chain amino acid aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,952,569	0	C	T	99.0%	340.9 / ‑4.5	100	A29V (GCG→GTG)	ilvE	branched‑chain amino acid aminotransferase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (51/48); total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 103 total aligned reads displayed.

AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT  >  NC_000913/3952472‑3952663

aGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACgtgc                                                                                              <  2:2209477/100‑1 (MQ=255)

 ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                             <  2:451003/100‑1 (MQ=255)

   aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                           <  1:1890793/100‑1 (MQ=255)

   aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                           <  1:1540691/100‑1 (MQ=255)

    atatAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCa                                                                                          <  2:1577476/100‑1 (MQ=255)

     tataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCAc                                                                                         <  2:2190050/100‑1 (MQ=255)

      ataAAAATGACCACGAAGAAAGCTGATTACATTTGGTACAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACt                                                                                        >  2:2820124/1‑100 (MQ=255)

       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                       >  1:2383979/1‑100 (MQ=255)

       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTACATGTGATGTCGCACGTGCTGCACTa                                                                                       >  2:886139/1‑100 (MQ=255)

         aaaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATg                                                                                     <  1:338426/100‑1 (MQ=255)

          aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATgg                                                                                    <  1:910415/100‑1 (MQ=255)

          aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATgg                                                                                    <  2:1540929/100‑1 (MQ=255)

           aaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGc                                                                                   <  2:2078655/100‑1 (MQ=255)

           aaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGc                                                                                   <  2:2350352/100‑1 (MQ=255)

            aTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCa                                                                                  >  2:651132/1‑100 (MQ=255)

                ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTc                                                                              >  2:679249/1‑100 (MQ=255)

                ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTc                                                                              >  2:2786773/1‑100 (MQ=255)

                  aCGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCgg                                                                            >  1:2475209/1‑100 (MQ=255)

                   cGAAGAAAGTTGATTACATTTGGGTCAATGGGGAGATGGTGCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCgg                                                                            <  2:2450981/99‑1 (MQ=255)

                   cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGt                                                                           >  1:815342/1‑100 (MQ=255)

                   cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGt                                                                           <  2:1217840/100‑1 (MQ=255)

                   cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGt                                                                           >  2:2853420/1‑100 (MQ=255)

                      agaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGtttt                                                                        <  2:1376743/100‑1 (MQ=255)

                      agaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGtttt                                                                        <  1:2636957/100‑1 (MQ=255)

                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                       >  2:906512/1‑100 (MQ=255)

                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                       <  2:47222/100‑1 (MQ=255)

                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                       <  2:1492499/100‑1 (MQ=255)

                        aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                      <  1:1002968/100‑1 (MQ=255)

                         aaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCGCTTCGGTTTTTGa                                                                     >  1:2541157/1‑100 (MQ=255)

                          aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGCTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGaa                                                                    <  2:1865206/100‑1 (MQ=255)

                          aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGaa                                                                    >  1:1812302/1‑100 (MQ=255)

                           gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                   >  1:876588/1‑100 (MQ=255)

                           gCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAg                                                                   <  2:948984/100‑1 (MQ=255)

                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                  <  2:3015332/100‑1 (MQ=255)

                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                  <  1:416228/100‑1 (MQ=255)

                             tGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGc                                                                 <  2:2026874/100‑1 (MQ=255)

                             tGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGc                                                                 <  2:818409/100‑1 (MQ=255)

                                ttACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATc                                                              <  1:1861440/100‑1 (MQ=255)

                                   cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                           >  1:2268230/1‑100 (MQ=255)

                                   cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                           <  2:570514/100‑1 (MQ=255)

                                      ttGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGc                                                        >  2:1117400/1‑100 (MQ=255)

                                        ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTa                                                      <  1:1855993/100‑1 (MQ=255)

                                         gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                     >  1:2728468/1‑100 (MQ=255)

                                         gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                     <  1:1463826/100‑1 (MQ=255)

                                           tCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGa                                                   >  1:276160/1‑100 (MQ=255)

                                            cAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGGATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGAc                                                  >  2:1626603/1‑100 (MQ=255)

                                             aaTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACt                                                 >  1:263203/1‑100 (MQ=255)

                                               tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCg                                               <  1:717691/100‑1 (MQ=255)

                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                              <  1:2604647/100‑1 (MQ=255)

                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                              >  1:1622251/1‑100 (MQ=255)

                                                 gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGca                                             >  1:844346/1‑100 (MQ=255)

                                                 gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGca                                             >  2:1113230/1‑100 (MQ=255)

                                                 gggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGAGATGTCGCACGTGCTGCACTATGGCACCTCGGTTTTTGAAGGCATCCGTTGCTACGAATCGca                                             >  2:2085238/1‑100 (MQ=255)

                                                  ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                            >  1:969471/1‑100 (MQ=255)

                                                  ggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcac                                            <  1:980657/100‑1 (MQ=255)

                                                   gagaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcaca                                           <  1:15371/100‑1 (MQ=255)

                                                   gagaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcaca                                           <  2:2841480/100‑1 (MQ=255)

                                                    agaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACaa                                          >  1:1435871/1‑100 (MQ=255)

                                                       tGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAgg                                       >  1:2068686/1‑100 (MQ=255)

                                                         gTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTGGCTACGACTCGCACAAAGGAc                                     >  2:345883/1‑100 (MQ=255)

                                                          ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                    <  1:1483430/100‑1 (MQ=255)

                                                            cGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCgg                                  >  2:805260/1‑100 (MQ=255)

                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                 >  1:1584112/1‑100 (MQ=255)

                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                 >  2:2771605/1‑100 (MQ=255)

                                                              cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                >  1:1106225/1‑100 (MQ=255)

                                                               tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                               <  2:2293361/100‑1 (MQ=255)

                                                                gggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                              <  1:33467/100‑1 (MQ=255)

                                                                 ggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTa                             <  1:2900402/100‑1 (MQ=255)

                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                            >  1:200268/1‑100 (MQ=255)

                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTgcat                            >  1:1214144/1‑97 (MQ=255)

                                                                    aGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTc                          >  1:2466244/1‑100 (MQ=255)

                                                                       cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                       >  1:1521078/1‑100 (MQ=255)

                                                                       cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                       >  1:3018444/1‑100 (MQ=255)

                                                                         cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                     <  1:906520/100‑1 (MQ=255)

                                                                         cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                     >  2:2919660/1‑100 (MQ=255)

                                                                         cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                     >  1:3032107/1‑100 (MQ=255)

                                                                              gTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTAGGTTTTCGAAGGCATCCGTTGCTTCGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGt                 <  1:1165393/99‑1 (MQ=255)

                                                                               tGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGa               >  1:1393877/1‑100 (MQ=255)

                                                                                gCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAg              >  1:2684951/1‑100 (MQ=255)

                                                                                 cATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGc             <  2:1393865/100‑1 (MQ=255)

                                                                                  aTGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCa            >  1:474868/1‑100 (MQ=255)

                                                                                  aTGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCa            <  1:2763132/100‑1 (MQ=255)

                                                                                  aTGTGATGTCGCACGTGCTGCACTATGGCACTACGGTTATTGAAGGCATCCGTTGCTACGACTCGCAAAAAGGACCGGATGTATTCCGCCATCGTGAgaa            <  2:445236/100‑3 (MQ=255)

                                                                                   tgtgTTTTCGCACGTGCTGCACTATCGCACTTCAGTTTTTGACGGCATCCGCGGCTACGACTCGCACAATCGACCGGTTGTAGTCCGCCATCGTGa               <  2:1956008/96‑1 (MQ=255)

                                                                                   tgtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat           >  1:832431/1‑100 (MQ=255)

                                                                                    gtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata          >  2:856773/1‑100 (MQ=255)

                                                                                     tgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat         >  2:1881238/1‑100 (MQ=255)

                                                                                     tgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCatat         >  1:243193/1‑100 (MQ=255)

                                                                                      gATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATg        >  2:1331100/1‑100 (MQ=255)

                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  1:132875/100‑1 (MQ=255)

                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  1:1113240/100‑1 (MQ=255)

                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  1:1534005/100‑1 (MQ=255)

                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  2:408141/100‑1 (MQ=255)

                                                                                       aTGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGc       <  2:1104017/100‑1 (MQ=255)

                                                                                         gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg     >  1:2679653/1‑100 (MQ=255)

                                                                                         gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg     <  2:2383957/100‑1 (MQ=255)

                                                                                         gTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAg     <  1:886147/100‑1 (MQ=255)

                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg   >  1:1547532/1‑100 (MQ=255)

                                                                                            gCACGTGCTGCACTATGGCACTTCGGTTTTTTTTTTTTTTTGTTGCTACGACTCGCACAAAAGACCGGTTGTATTCCGCCATCGTGAGCATATGCa      >  2:1658976/1‑96 (MQ=255)

                                                                                            gCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt  >  1:935637/1‑100 (MQ=255)

AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGT  >  NC_000913/3952472‑3952663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A