Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F125 I0 R1 65 108.6 5319566 96.1% 5112102 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,952,569 C→T 82.4% A29V (GCG→GTG)  ilvE → branched‑chain amino acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,952,5690CT82.4% 239.0 / 39.6 109A29V (GCG→GTG) ilvEbranched‑chain amino acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (10/9);  new base T (38/51);  total (49/60)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA  >  NC_000913/3952472‑3952668
                                                                                                 |                                                                                                   
aGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACgtgc                                                                                                   <  1:1731129/100‑1 (MQ=255)
 ggAATATACAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                                  <  2:914892/100‑1 (MQ=255)
 ggAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCt                                                                                                  >  2:600561/1‑100 (MQ=255)
  gAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTg                                                                                                 >  2:1367045/1‑100 (MQ=255)
  caaTATAAAAATGACCACGAATAAAGCTGATTACATTTGGTTCAAAGGGGAGATGGTTCGCTGCGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTg                                                                                                 >  2:1615853/2‑100 (MQ=255)
   aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                                <  2:237765/100‑1 (MQ=255)
   aaTATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGtgctgc                                                                                                <  2:1848084/100‑1 (MQ=255)
     tataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCAc                                                                                              <  1:554595/100‑1 (MQ=255)
     tataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCAc                                                                                              <  1:803705/100‑1 (MQ=255)
     tataAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGCGGCATGTGATGTCGCACATGCTGCAc                                                                                              >  2:747110/1‑100 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                            >  2:1985986/1‑100 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                            >  1:2420884/1‑100 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTa                                                                                            >  2:2550389/1‑100 (MQ=255)
       taAAAATGACCACGAAGAAAGCTGATTACAGTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTa                                                                                            >  2:1738002/1‑100 (MQ=255)
         aaaaTGACCACGTAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTTATGTCGCACGCGCTGCACTATg                                                                                          <  2:964711/100‑1 (MQ=255)
          aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATg                                                                                          >  2:2235456/1‑99 (MQ=255)
          aaaTGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATg                                                                                          <  1:2305496/99‑1 (MQ=255)
                ccACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTc                                                                                   >  2:2293462/1‑100 (MQ=255)
                ccACGAAGAAAGCTGATTACATCTGGTTCCCTGGGGAGATGATTCGCTAAGAAGAAACAAAGGAACATGTAATGTCGCAAGAACTGCACTATGGCACTTc                                                                                   >  1:2081703/1‑100 (MQ=255)
                 cACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCg                                                                                  >  2:1407608/1‑100 (MQ=255)
                 cACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGATCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGATGGACTATgg                                                                                         >  2:1306953/1‑93 (MQ=255)
                  aCGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCgg                                                                                 <  1:1678832/100‑1 (MQ=255)
                   cGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGt                                                                                >  1:148307/1‑100 (MQ=255)
                      agaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGtttt                                                                             <  1:2511892/100‑1 (MQ=255)
                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                            <  2:1134457/100‑1 (MQ=255)
                       gaaAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGttttt                                                                            <  2:1974031/100‑1 (MQ=255)
                        aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                           <  1:2224021/100‑1 (MQ=255)
                        aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                           <  2:2368215/100‑1 (MQ=255)
                        aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTg                                                                           <  1:227293/100‑1 (MQ=255)
                        aaaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGttttt                                                                            <  2:595018/99‑1 (MQ=255)
                         aaGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGa                                                                          <  1:1560896/100‑1 (MQ=255)
                          aGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGaa                                                                         <  1:175612/100‑1 (MQ=255)
                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                       <  1:1556087/100‑1 (MQ=255)
                            cTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                       <  1:1407622/100‑1 (MQ=255)
                             tGATTATATTTGGTTCAATGGGGAGATGGTTCGCTGAGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAgg                                                                       <  1:2351560/99‑1 (MQ=255)
                              gATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGAAGAGAAGGTGCATGTGATGTCGCACGTGGTGCACTATGGCACTTCGGTTTTTGAAgg                                                                       >  2:1149238/1‑98 (MQ=255)
                                ttACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATc                                                                   <  1:947634/100‑1 (MQ=255)
                                  aCATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCg                                                                 >  1:1405114/1‑100 (MQ=255)
                                   cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                >  2:156714/1‑100 (MQ=255)
                                   cATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGt                                                                >  1:2225284/1‑100 (MQ=255)
                                       tGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCt                                                            <  2:2210389/100‑1 (MQ=255)
                                        ggTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCt                                                            <  2:2420854/99‑1 (MQ=255)
                                         gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTAc                                                          <  2:2650129/100‑1 (MQ=255)
                                         gTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTAAGGAACTTCGGATTTTGAAGGCATCCGTTGCTAc                                                          >  1:737848/1‑100 (MQ=255)
                                         gTTCAATCGGGTGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGTTTTTTGAAGACATCCGTTTCTAc                                                          >  2:2392918/1‑100 (MQ=255)
                                              aTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                     >  2:214720/1‑100 (MQ=255)
                                              aTGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTc                                                     >  2:763113/1‑100 (MQ=255)
                                               tGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCg                                                    <  2:963274/100‑1 (MQ=255)
                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   <  1:1673924/100‑1 (MQ=255)
                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   >  2:1659237/1‑100 (MQ=255)
                                                ggggAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGc                                                   >  2:1777125/1‑100 (MQ=255)
                                                 gtgcgaTGGTTCGCTGGGAAGCCGCGAAGTTGCATGTGATGTCGCACTTGCTGCACTATGGCACTTCGATTTTTGAAGGCATCCCTTGCTAGGACTCGc                                                   <  2:1380360/95‑1 (MQ=255)
                                                   gagaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcaca                                                >  1:2622224/1‑100 (MQ=255)
                                                   gagaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGcaca                                                <  2:956523/100‑1 (MQ=255)
                                                     gaTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTTCGACTCGcacaca                                              <  1:1797361/100‑3 (MQ=255)
                                                      aTGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAg                                             <  1:2083253/100‑1 (MQ=255)
                                                          ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                         >  2:502962/1‑100 (MQ=255)
                                                          ttCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAcc                                         <  2:1431388/100‑1 (MQ=255)
                                                           tCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCg                                        <  1:723619/100‑1 (MQ=255)
                                                            cGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCgg                                       <  1:623785/100‑1 (MQ=255)
                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                      <  2:1844283/100‑1 (MQ=255)
                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                      <  2:1088677/100‑1 (MQ=255)
                                                             gCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGt                                      <  1:228356/100‑1 (MQ=255)
                                                              cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                     >  2:2012773/1‑100 (MQ=255)
                                                              cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                     <  1:2102401/100‑1 (MQ=255)
                                                              cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGAACGGtt                                     <  2:556845/100‑1 (MQ=255)
                                                              cTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTAAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGtt                                     >  2:1445416/1‑100 (MQ=255)
                                                               tGGGAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                                    <  1:2165756/100‑1 (MQ=255)
                                                               ctggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTg                                    <  1:1546916/98‑1 (MQ=255)
                                                                gggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGt                                   <  1:1620986/100‑1 (MQ=255)
                                                                 ggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTgag                                  >  2:1546901/1‑98 (MQ=255)
                                                                 ggAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTgag                                  >  2:1545265/1‑98 (MQ=255)
                                                                 ggAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATAGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTa                                  <  1:2270150/100‑1 (MQ=255)
                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                 >  2:167693/1‑100 (MQ=255)
                                                                  gAAGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAt                                 >  1:1307651/1‑100 (MQ=255)
                                                                   aaGACGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTAtt                                >  1:877177/1‑100 (MQ=255)
                                                                     gACGCGAAGGTGCATGTGATGTCGCACGCGCTGTACTATGGTATTTTGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTcc                              <  1:181379/100‑1 (MQ=255)
                                                                      aCGCGAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCg                             <  1:950917/100‑1 (MQ=255)
                                                                       cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            <  1:1539078/100‑1 (MQ=255)
                                                                       cgcgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            <  1:2235483/100‑1 (MQ=255)
                                                                       cgcgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            >  1:1192340/1‑100 (MQ=255)
                                                                       cgcgAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGc                            <  1:673486/100‑1 (MQ=255)
                                                                         cgAAGGTGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCa                          >  2:1999601/1‑100 (MQ=255)
                                                                               tGCATGTGATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGa                    >  1:590840/1‑100 (MQ=255)
                                                                                 cATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGACATACGTTGCTACGACTAACACAAAGTACCGGTTGTATTCCGCCATCGTGAGc                  >  1:1379890/1‑100 (MQ=255)
                                                                                   tgtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTTGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat                <  2:1410574/100‑1 (MQ=255)
                                                                                   tgtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCat                >  1:282239/1‑100 (MQ=255)
                                                                                    gtgATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACATAGGACCGGTTGTATTCCGCCATCGTGAGCata               <  1:2514912/100‑1 (MQ=255)
                                                                                    gtgATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCata               >  1:1035789/1‑100 (MQ=255)
                                                                                      gATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATg             <  1:2178065/100‑1 (MQ=255)
                                                                                      gATGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATg             >  2:759420/1‑100 (MQ=255)
                                                                                        tGTCGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCa           >  1:1648498/1‑100 (MQ=255)
                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        <  1:2066848/100‑1 (MQ=255)
                                                                                           cGCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCg        <  2:146439/100‑1 (MQ=255)
                                                                                            gCACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCCTTGCTACGCCTCGAACAAAGGACCGGTTATATTCCGCCACCGTGCGAatacgaacccc       <  2:1150456/100‑9 (MQ=255)
                                                                                            gCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGt       >  2:2187789/1‑100 (MQ=255)
                                                                                             cACGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc      >  2:1024826/1‑100 (MQ=255)
                                                                                             cACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTc      >  2:1899372/1‑100 (MQ=255)
                                                                                              aCGTGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCt     <  1:246955/100‑1 (MQ=255)
                                                                                                 cgcTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCa  >  2:289187/1‑100 (MQ=255)
                                                                                                 |                                                                                                   
AGGAATATAAAAATGACCACGAAGAAAGCTGATTACATTTGGTTCAATGGGGAGATGGTTCGCTGGGAAGACGCGAAGGTGCATGTGATGTCGCACGCGCTGCACTATGGCACTTCGGTTTTTGAAGGCATCCGTTGCTACGACTCGCACAAAGGACCGGTTGTATTCCGCCATCGTGAGCATATGCAGCGTCTGCA  >  NC_000913/3952472‑3952668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A