Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F181 I1 R1 66 119.2 5981814 94.9% 5676741 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.93.8% 515.2 / 31.0 130intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (8/0);  new base . (53/67);  total (63/67)
Fisher's exact test for biased strand distribution p-value = 2.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 146 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACA  >  NC_000913/4409946‑4410138
                                                                                                   |                                                                                             
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                               <  2:115191/100‑1 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                            >  1:403170/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                         <  2:2835708/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                         >  1:1865503/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                        <  2:12498/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                        >  1:349684/1‑100 (MQ=255)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                       >  1:2659979/1‑99 (MQ=255)
         ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgt                                                                                      <  2:2319103/100‑3 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCt                                                                                   >  2:2934335/1‑100 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCta                                                                                  <  1:1996919/100‑1 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCtat                                                                                 <  1:751243/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCt                                                                             <  1:93253/100‑1 (MQ=255)
                  gACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTg                                                                            >  2:2519951/1‑100 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTg                                                                            >  2:2506254/1‑99 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTgga                                                                          >  1:2312237/1‑99 (MQ=255)
                     cGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTa                                                                         <  2:746819/100‑1 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAg                                                                       <  2:409603/100‑1 (MQ=255)
                        aaTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATGTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAg                                                                       <  2:661782/99‑1 (MQ=255)
                            tGCTTATTTGAAGCGGTGCGCCAGCGAAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                  >  1:1818169/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                 >  1:1741836/1‑99 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  2:247640/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  1:562783/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  2:2786640/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  2:1675774/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                <  1:1023668/100‑1 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  2:2854073/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                >  2:47532/1‑100 (MQ=255)
                               ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                               <  1:1698399/100‑1 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                              <  1:2063187/100‑1 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                             <  1:538207/100‑1 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                             >  1:481098/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                            >  2:501588/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                            >  1:1602469/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                            <  2:628490/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                          <  1:1011640/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                          >  2:67527/1‑100 (MQ=255)
                                     gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTg                                                         >  2:1104663/1‑100 (MQ=255)
                                       aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCa                                                       <  2:2821033/100‑1 (MQ=255)
                                       aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCa                                                       <  2:146289/100‑1 (MQ=255)
                                         cGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                     >  1:1318841/1‑100 (MQ=255)
                                         cGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                     <  1:1123931/100‑1 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                    >  1:2142590/1‑100 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                     >  1:1206073/1‑98 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                   <  1:1031051/100‑1 (MQ=255)
                                             gcgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                 <  1:1984705/100‑1 (MQ=255)
                                             gcgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                 <  2:174956/100‑1 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                               >  2:1091118/1‑100 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                              <  1:750351/100‑1 (MQ=255)
                                                   gtgcAGCTAATTTGTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtttt                                           >  1:2018054/3‑100 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                         <  2:1452011/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTTCAAATATTCATTttatt                                        >  2:2698428/1‑100 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                        <  2:538858/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                        <  2:2213987/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                        <  1:1295889/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                        <  2:2164701/100‑1 (MQ=255)
                                                       aGCTAATTTCTCAGCATTTAGAAAAGCGTTCCAGTTTAAGTTGG‑TTTTTTTTGGCTATAGCTGGTAATCTAATTACGTATTGCAAATATTCAtt                                             <  2:1927862/94‑1 (MQ=255)
                                                       aGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                       <  1:704098/100‑1 (MQ=255)
                                                       aGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                       <  1:2854089/100‑1 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                      >  1:2468831/1‑100 (MQ=255)
                                                        ccgatcttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                      <  1:1889091/94‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                    <  1:2322766/100‑1 (MQ=255)
                                                            aTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                  <  2:641991/100‑1 (MQ=255)
                                                            aTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                  >  2:1821928/1‑100 (MQ=255)
                                                             tttattataaTTATGAAAAGACATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGTTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                  <  2:2533729/91‑1 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                 >  2:2315396/1‑100 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                  <  2:1984815/99‑1 (MQ=255)
                                                              ttctttgatttATGAAAAGCCATCCATATTTGGATGG‑TTTTTTTTATCTATAGTTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                <  2:2598958/91‑1 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                              >  2:2161029/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                              >  1:1653307/1‑100 (MQ=255)
                                                                 tcATAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                              <  2:2216302/99‑1 (MQ=255)
                                                                 tcAGAATTATGAAAAGCAATTCAGATTTGGATGG‑TTTTGTTTGTCTATAGCTGGTAATATAATTACGTATTGCTATTATTCATTTTTTTAAGATTTaa                               >  2:2595239/1‑98 (MQ=255)
                                                                  ctgtaTTATGCAAAGCCATACAGATTTGTATGG‑TTTTTTTTGTCTATAGCTGGAAAGATAATTACGTATGGCAAATATTCATTTTATTAATATTTAAACt                            <  2:1956909/96‑1 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                            <  1:156466/100‑1 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                            >  2:2103904/1‑100 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                            >  1:2781433/1‑100 (MQ=255)
                                                                   aGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                           >  2:289337/1‑100 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                       <  2:2468816/100‑1 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                       <  1:2352503/100‑1 (MQ=255)
                                                                       acaaaaaaaACCATCCAAATCTGGATGGCTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                        >  1:363628/6‑100 (MQ=37)
                                                                        tATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTT‑TCTATATCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                      >  2:2960787/1‑100 (MQ=255)
                                                                        tATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                      <  2:1865492/100‑1 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGGCTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                       >  1:2183423/5‑100 (MQ=37)
                                                                         aTGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                     >  1:1269498/1‑100 (MQ=255)
                                                                         aTGAAAAGCCATCCAGATTTGGATGG‑‑TTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                    >  1:965139/1‑100 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATTtat                    >  2:1157747/1‑100 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                  >  2:944277/1‑100 (MQ=255)
                                                                            aaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                  >  1:2629924/1‑100 (MQ=255)
                                                                              aaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                <  2:1318834/100‑1 (MQ=255)
                                                                               aGCCATCCAGATTTGGTTGG‑TTTTTTTTATCTTTAGCTTATAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                 <  2:617062/98‑1 (MQ=255)
                                                                               aGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                <  2:1908945/99‑1 (MQ=255)
                                                                                    tCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat          <  1:2321625/100‑1 (MQ=255)
                                                                                      cAGATTTTGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat          >  2:72023/1‑98 (MQ=255)
                                                                                      cAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat        >  1:1879549/1‑100 (MQ=255)
                                                                                       aGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata         <  2:228374/98‑1 (MQ=255)
                                                                                       aGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata       <  2:2285244/100‑1 (MQ=255)
                                                                                       aGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata       >  1:107630/1‑100 (MQ=255)
                                                                                         aTTTGGCTGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTAAGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata       <  2:2084514/98‑1 (MQ=255)
                                                                                         aTTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg     <  2:562780/100‑1 (MQ=255)
                                                                                         aTTTGGATGG‑CTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg     <  2:1955153/100‑1 (MQ=255)
                                                                                               aTGGTTTTTTTTTTTCTTTAACTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGTAATAATTTATGata  >  2:1765111/1‑96 (MQ=255)
                                                                                                   |                                                                                             
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACA  >  NC_000913/4409946‑4410138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A