Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I1 R1 59 169.8 8365175 96.8% 8097489 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.96.3% 797.0 / 21.9 188intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (5/2);  new base . (90/91);  total (95/93)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 215 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140
                                                                                                   |                                                                                               
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                                 >  1:594822/1‑100 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                              >  2:1998277/1‑100 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATATGAAGAGGTGCGCCAGCGCAGCTAATTTCGCAGGATTATGAACGGCCATCCAGATTTAGATGGtttttt                                                                                            >  2:3363431/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                           <  2:1660189/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                           <  1:2425357/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                          >  2:615994/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGATGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                          >  1:2955084/1‑100 (MQ=255)
        tttCGGTTGGTACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcata                                                                                         <  2:805305/100‑5 (MQ=25)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                         <  1:2730746/100‑2 (MQ=255)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                         <  2:3213743/100‑2 (MQ=255)
         ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgt                                                                                        >  1:631061/1‑98 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       <  1:967219/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       <  2:3588663/100‑4 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       >  2:1559448/1‑95 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCtat                                                                                   <  2:1930753/100‑1 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCtat                                                                                   <  2:157789/100‑1 (MQ=255)
               tGCGACCGGAATTTGTGTAGTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCAATCCAGATTTGGATGG‑TTTTTTTTGTCTATAg                                                                                 <  2:2430162/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGTCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCt                                                                               <  1:2029685/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCt                                                                               >  1:2765891/1‑100 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGt                                                                            <  2:1448118/100‑1 (MQ=255)
                     cGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTa                                                                           <  2:764264/100‑1 (MQ=255)
                      ggAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGtt                                                                           >  2:3086001/1‑98 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGAAGG‑TTTTTTTTGTCTATAGCTGGTAAg                                                                         <  2:3773863/100‑1 (MQ=255)
                         aTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGAt                                                                       >  1:1291103/1‑100 (MQ=255)
                          tttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTGTTTGTCTATAGCTGGTAAGATa                                                                      <  2:1286964/100‑1 (MQ=255)
                               ttATTTGAAGCGGTGCGCCAGCGCAGATAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                                 >  2:738292/1‑100 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                               >  1:4010866/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                              >  1:120473/1‑100 (MQ=255)
                                   ttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAt                                                             <  2:2136603/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                            >  1:296988/1‑100 (MQ=255)
                                     naaGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAANGCCNTCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTNCGTNTTg                                                           >  1:766429/2‑100 (MQ=255)
                                     gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTg                                                           >  1:1387420/1‑100 (MQ=255)
                                     gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTg                                                           <  2:2088743/100‑1 (MQ=255)
                                         cGGTGCTCCAGCGCTGTTAATTTCTCTGAATTTTAAAAAGCCATTGAGATTTGGATGG‑TTTTTTTGGTCTATAGCTGGTAAGATATTTACGTTTTGCa                                                         <  2:3066039/98‑1 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                      >  1:512504/1‑100 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                      <  2:477117/100‑1 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                     >  2:2397199/1‑100 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                     <  1:891449/100‑1 (MQ=255)
                                             gcgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                   >  1:726853/1‑100 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                                <  1:2264955/100‑1 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               <  2:570432/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                          <  2:2804875/100‑1 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                        >  2:1864283/1‑100 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                        >  2:826444/1‑100 (MQ=255)
                                                         cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                       <  2:4134569/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                      <  2:2565947/100‑1 (MQ=255)
                                                              ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTAATTTTATTAATATTTa                                  >  2:3441583/1‑100 (MQ=255)
                                                              ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                  <  1:3484703/100‑1 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                                 >  1:1277041/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  2:3708181/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  2:2388989/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                <  1:2291986/100‑1 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  1:3224780/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  1:3650223/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  1:1719138/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                <  1:1485222/100‑1 (MQ=255)
                                                                ctcAGAATCATAAAAAGCCATCCAGATTGGGATGG‑TTTTTTTTGTCTATAACTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                >  2:1621193/1‑100 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                               <  1:2907835/100‑1 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                              >  2:595429/1‑100 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                              >  2:2678886/1‑100 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                              <  2:1177314/100‑1 (MQ=255)
                                                                    gAATTATGAATAGCCATCCAGATTTGTTTGGTTTTTTTTT‑TCTATAGCTGTTAAGATAATTACATATTGCAAATATTCATTTTATTAATATTTaaa                                <  2:196260/96‑1 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                            >  1:1041352/1‑100 (MQ=255)
                                                                     aaTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTaa                           <  1:2631478/100‑1 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                          >  1:1936203/1‑100 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                         <  2:1145285/100‑1 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                         <  1:1864336/100‑1 (MQ=255)
                                                                        tATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        <  2:353628/100‑1 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        >  2:4040056/5‑100 (MQ=38)
                                                                         aTGAAAAGCCATCTAGATTTGTATGG‑‑TTTTTTTGTTTATAGCTGTTAAAATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                      <  2:638233/100‑1 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGG‑TTTTTGTTGTCTATATCTGTTAAGATAATTACGTATTGAAAATATTAATTTTATTAATATTTAAACTTAATCtat                      <  2:1010579/100‑1 (MQ=255)
                                                                          tGAAAAGCCATCCAAATAAGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATCACACATTGCAACCATTCCTTTTATTAATCATAAAACCTAATc                         >  2:4161888/1‑97 (MQ=255)
                                                                          aaaaaaACCATCCAAATCTGGATGG‑CTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                      >  1:2513376/3‑100 (MQ=37)
                                                                           gAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtata                     <  1:926972/100‑1 (MQ=255)
                                                                           gAAAAGCCATCCAGATTTGGATGG‑TATTTATTGTATATGTCTCAGAACATAATGACATTTTGTAAATATTAATTGTATTCTTATTTAAACTTattgga                       >  2:1443901/1‑93 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                    >  2:4114813/1‑100 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                    >  2:289516/1‑100 (MQ=255)
                                                                              aaaccATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtata                     >  2:2409502/4‑97 (MQ=255)
                                                                              aaGCCATCCAGATTTGGATGGTTTTTTTTT‑TTTTTTTTTGGTAAGATAATTACGTATTGCAATTTTTCATTTTATTAATATTTAAACTTTATCTatat                    >  2:3918821/1‑98 (MQ=255)
                                                                              aaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                  <  2:2221624/100‑1 (MQ=255)
                                                                                gCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa                >  1:224040/1‑100 (MQ=255)
                                                                                gCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa                <  2:2703382/100‑1 (MQ=255)
                                                                                   aTCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataa             >  1:4066313/1‑100 (MQ=255)
                                                                                    tCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat            <  2:2159155/100‑1 (MQ=255)
                                                                                     ccAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata           <  1:3111718/100‑1 (MQ=255)
                                                                                     ccAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata           <  1:2014942/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          <  2:3975897/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          >  1:899756/1‑100 (MQ=255)
                                                                                      cAGATTTGGATAG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          <  2:3038790/100‑1 (MQ=255)
                                                                                       aaatctGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata         >  2:3521744/6‑100 (MQ=255)
                                                                                        gATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat        <  1:3620096/100‑1 (MQ=255)
                                                                                        gATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat        <  1:4174023/100‑1 (MQ=255)
                                                                                        gATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat        <  2:2290372/100‑1 (MQ=255)
                                                                                           ttGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc     <  1:1998292/100‑1 (MQ=255)
                                                                                           ttGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc     <  2:2614934/100‑1 (MQ=255)
                                                                                           ttGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc     <  1:1135553/100‑1 (MQ=255)
                                                                                            tGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACa    >  1:2804867/1‑100 (MQ=255)
                                                                                             ggATGGTTTTTTTTT‑TTTTTTTCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAt   >  2:2601326/1‑100 (MQ=255)
                                                                                             ggATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAGTAATATATGAcgt   <  1:2837329/100‑3 (MQ=255)
                                                                                              gATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATg  <  1:1752197/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A