Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.95.7% 572.2 / 18.4 141intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base . (75/59);  total (80/61)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAT  >  NC_000913/4409946‑4410139
                                                                                                   |                                                                                              
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                                >  1:2844051/1‑100 (MQ=255)
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                                >  1:54541/1‑100 (MQ=255)
 cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                               >  2:1979820/1‑100 (MQ=255)
 cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                               >  1:652731/1‑100 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttt                                                                                           <  1:42833/100‑1 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttt                                                                                           <  2:2224548/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGCATTATGAAAAACCATCCAAATCTGGATGGcttttca                                                                                          <  2:2047441/100‑3 (MQ=37)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                          >  2:2593456/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                          <  2:2145086/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                         <  1:2380062/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                         >  2:1808857/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                         <  1:1260776/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                         <  1:1427975/100‑4 (MQ=37)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                      <  1:1543462/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                      <  2:1871865/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                      <  2:1585931/100‑4 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCta                                                                                   >  1:427083/1‑100 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCta                                                                                   >  2:2587161/1‑100 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataattct                                                                                   <  1:1373014/100‑10 (MQ=25)
                gCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGc                                                                               <  2:1114245/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCt                                                                              <  2:1971511/100‑1 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGt                                                                           >  2:1423748/1‑100 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGt                                                                           >  1:1344211/1‑100 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGt                                                                           <  1:490555/100‑1 (MQ=255)
                     cGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTa                                                                          >  1:814757/1‑100 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAg                                                                        <  1:36882/100‑1 (MQ=255)
                            tGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                   >  2:1943687/1‑100 (MQ=255)
                            tGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                   >  1:2505488/1‑100 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                  <  2:623056/100‑1 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                  <  1:1342328/100‑1 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                 >  2:943219/1‑100 (MQ=255)
                               ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                                <  1:1613255/100‑1 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTTGTAAGATAATTAc                                                                >  1:2908007/1‑99 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                               <  2:112640/100‑1 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                              >  1:923485/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                             <  1:1663533/100‑1 (MQ=255)
                                   ttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAt                                                            <  1:1816304/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                           <  1:2612384/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTGTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                           >  2:2762633/1‑100 (MQ=255)
                                       aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCa                                                        <  1:159287/100‑1 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                     <  1:1183732/100‑1 (MQ=255)
                                          ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGAAAat                                                     >  1:845506/1‑100 (MQ=255)
                                              cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                  >  2:2352417/1‑100 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                <  2:346944/100‑1 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                <  2:2146692/100‑1 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                               >  2:265201/1‑100 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                               >  1:2479823/1‑100 (MQ=255)
                                                 naGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                              >  2:550524/2‑100 (MQ=255)
                                                   gcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtttt                                            <  2:1047328/100‑1 (MQ=255)
                                                    cgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑‑TTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                          <  2:2446568/100‑1 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                          <  2:54543/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                         <  1:1954996/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                         <  2:1494764/100‑1 (MQ=255)
                                                         cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                      >  2:814552/1‑100 (MQ=255)
                                                         cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                      <  1:2141651/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTTTAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                     <  1:1613781/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                     >  2:1469484/1‑100 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑GTGTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                       >  2:1801659/1‑98 (MQ=255)
                                                          tAATTACGCAGAATTATGAATAGCTATCCAAAAATGGATGG‑TTTTTTTTGTCTAGAGCTTGTAAGATGATGATGGATTGCAAATATTCATTTTATTaaga                                     <  1:2774357/100‑3 (MQ=255)
                                                           aaTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                    >  2:2758331/1‑100 (MQ=255)
                                                            aTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                   >  2:2534269/1‑100 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATATCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                  >  1:1771605/1‑100 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTAATTTTATTAATAttt                                  >  1:1292759/1‑100 (MQ=255)
                                                              ttCTCTGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                  <  1:398076/99‑1 (MQ=255)
                                                              ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                 <  2:2121442/100‑1 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                             <  1:1809815/100‑1 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATATCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                              <  1:1095799/97‑1 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATCACGTATAGCAAATATTCATTTTATTAATATTTAAACTTa                           >  1:2039381/1‑100 (MQ=255)
                                                                     aaTTATGAAAAGACATCCAGATTTGGATTG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                             <  1:150874/97‑1 (MQ=255)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         >  2:1727686/7‑100 (MQ=37)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         <  2:2893755/100‑1 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         >  1:747009/1‑100 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         >  1:189696/1‑100 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         <  1:2324517/100‑1 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                        >  2:1202424/1‑100 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGGCTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                         >  1:1157265/5‑99 (MQ=37)
                                                                        caaaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                       >  2:1239664/5‑100 (MQ=38)
                                                                        caaaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                       >  1:1222806/5‑100 (MQ=38)
                                                                          tGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                     >  2:2568869/1‑100 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                     >  2:1458219/1‑100 (MQ=255)
                                                                          aaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATGTTTAAATTTAATCtat                     >  1:1702918/3‑100 (MQ=37)
                                                                          aaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                     >  2:2483843/3‑100 (MQ=38)
                                                                          aaaaaaACCATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                     >  2:468189/3‑100 (MQ=38)
                                                                          aaaaaaACCATCCAAATCTGGATGG‑CTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                     >  2:657170/3‑100 (MQ=37)
                                                                             aaaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                  <  1:2067531/100‑1 (MQ=255)
                                                                             aaaGCCATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                  <  1:1783652/100‑1 (MQ=255)
                                                                              aaaccATCCAAATCTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                 >  2:1336310/4‑100 (MQ=255)
                                                                                  cATCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaata             >  2:2792080/1‑100 (MQ=255)
                                                                                   aTCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataa            >  1:2708779/1‑100 (MQ=255)
                                                                                    tCCAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat           >  2:2757349/1‑100 (MQ=255)
                                                                                     ccAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata          <  1:2587128/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat         <  2:868913/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGG‑CTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata          <  1:1979792/99‑1 (MQ=255)
                                                                                       ngATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTAATTTTATTAATATTTAAACTTAATCTATATGGAATAatata        >  1:1345582/2‑100 (MQ=255)
                                                                                        gATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat       <  1:265197/100‑1 (MQ=255)
                                                                                        gATTTGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat       <  1:2780422/100‑1 (MQ=255)
                                                                                        aatctGGAT‑GTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTGATATTTAAACTTAATCTATATGGAATAatatat       <  1:1902324/96‑1 (MQ=255)
                                                                                           ttGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc    <  2:747014/100‑1 (MQ=255)
                                                                                            tGGATGG‑TTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACa   <  1:2906148/100‑1 (MQ=255)
                                                                                              gATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAt  <  1:669084/100‑1 (MQ=255)
                                                                                                   |                                                                                              
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAT  >  NC_000913/4409946‑4410139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A