Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F183 I0 R1	119	96.4	4778198	94.0%	4491506	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,542,503	G→A	100%	W699* (TGG→TGA)	feoB →	ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,542,503	0	G	A	96.8%	288.8 / ‑0.0	94	W699* (TGG→TGA)	feoB	ferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (48/43); total (50/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

CCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGG  >  NC_000913/3542407‑3542597

ccTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgaggg                                                                                             >  1:693891/1‑100 (MQ=255)

ccTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgaggg                                                                                             >  1:1914861/1‑100 (MQ=255)

 cTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGc                                                                                            <  1:2268881/100‑1 (MQ=255)

     ttttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGaa                                                                                        >  2:158257/1‑100 (MQ=255)

     ttttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGaa                                                                                        <  1:141618/100‑1 (MQ=255)

     ttttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGCGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGaa                                                                                        >  2:1000808/1‑100 (MQ=255)

       ttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAata                                                                                      >  2:1874882/1‑100 (MQ=255)

       ttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAata                                                                                      >  2:1874984/1‑100 (MQ=255)

         cGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATc                                                                                    >  2:1772554/1‑100 (MQ=255)

           tCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGc                                                                                  <  2:1914845/100‑1 (MQ=255)

            ccTGCTGTATGTACCATGTATCTCGGTGATTGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGc                                                                                  <  1:1175069/99‑1 (MQ=255)

            ccTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCt                                                                                 >  2:500530/1‑100 (MQ=255)

            ccTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCt                                                                                 <  1:2182330/100‑1 (MQ=255)

             ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCtt                                                                                >  2:280872/1‑100 (MQ=255)

             ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCtt                                                                                >  1:715061/1‑100 (MQ=255)

                ctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACt                                                                             >  2:1122906/1‑100 (MQ=255)

                 tgtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTc                                                                            >  2:111682/1‑100 (MQ=255)

                 tggctgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTCCTCCATCCTGTGAGGGCTGAATATCGCtn                                                                                <  2:393270/92‑2 (MQ=255)

                  gtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCa                                                                           >  2:689326/1‑100 (MQ=255)

                    atgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACt                                                                         >  1:1645846/1‑100 (MQ=255)

                    atgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACt                                                                         <  1:1575696/100‑1 (MQ=255)

                     tgtaCCGTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTg                                                                        <  2:2171686/100‑1 (MQ=255)

                       taCCATGTATCTCGGTGATGGTGGCTATATCAAATAAATCAAGCCATAGCTGGATGGTCTTCTTCATACTGTAAGTACTTAATATCTTTTACTCa                                                                           >  2:324265/1‑95 (MQ=255)

                       taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGc                                                                      >  1:1518278/1‑100 (MQ=255)

                        aCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCa                                                                     >  2:1218304/1‑100 (MQ=255)

                          cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   >  2:1071652/1‑100 (MQ=255)

                          cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   >  2:735504/1‑100 (MQ=255)

                          cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   >  2:413426/1‑100 (MQ=255)

                           aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   <  1:1576140/99‑1 (MQ=255)

                           aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACa                                                                  <  1:1465411/100‑1 (MQ=255)

                              tATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTg                                                               <  2:1778946/100‑1 (MQ=255)

                               aTCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGt                                                              >  1:1014283/1‑100 (MQ=255)

                                tctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGtt                                                             >  2:1512135/1‑100 (MQ=255)

                                 ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                            <  1:740161/100‑1 (MQ=255)

                                  tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                            <  2:1381238/99‑1 (MQ=255)

                                   cGGTGATGGGTGCTATCGCCCGTGAATCGAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                            <  2:1380244/98‑1 (MQ=255)

                                   cGGTGATGGGGGCTATCGTCCGAGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTATGAGAGGCGAATCTCGCCTACTCACTGGCAACATCGttat                                                           >  2:2155823/1‑97 (MQ=255)

                                   cGGTGATGAGGGTTATCGCCCGTGAATCGAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACGGGCAACATTGTTc                                                            <  2:2174575/98‑1 (MQ=255)

                                     gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        <  1:410838/100‑1 (MQ=255)

                                     gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        <  2:68137/100‑1 (MQ=255)

                                     gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        <  1:2031680/100‑1 (MQ=255)

                                        aTGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAgg                                                     >  1:1766764/1‑100 (MQ=255)

                                        aTGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAgg                                                     >  1:1731345/1‑100 (MQ=255)

                                          gggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTc                                                   <  1:1598922/100‑1 (MQ=255)

                                          gggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTc                                                   >  1:625415/1‑100 (MQ=255)

                                          gggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACCGGCAACATTGTTCTATCAGGTc                                                   >  1:2114341/1‑100 (MQ=255)

                                           ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTc                                                   >  2:2303035/1‑99 (MQ=255)

                                           ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  2:1332060/1‑100 (MQ=255)

                                           ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  2:1552585/1‑100 (MQ=255)

                                           ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  1:639053/1‑100 (MQ=255)

                                           ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  1:1289607/1‑100 (MQ=255)

                                            gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                 <  1:835269/100‑1 (MQ=255)

                                             ggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCCTCTCCATCCTGCCAGGGCTGAATATCGCTTACTCACTGGCCACATTGCTCTACCAGGTCGcc                                                >  2:98951/1‑100 (MQ=255)

                                              gCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCa                                               <  1:640471/100‑1 (MQ=255)

                                              gCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCa                                               <  1:637818/100‑1 (MQ=255)

                                               cgaTCGCCGGTGAGTCAAGCGGTGGCTTGATGGTCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTAGCAGGTCGCCAg                                              <  2:2195866/98‑1 (MQ=255)

                                               cTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAg                                              <  2:1518268/100‑1 (MQ=255)

                                                tATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGc                                             <  1:2069466/100‑1 (MQ=255)

                                                 aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAg                                              <  2:2138779/98‑1 (MQ=255)

                                                     cccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAg                                        <  1:1171404/100‑1 (MQ=255)

                                                       cGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTc                                      >  1:510873/1‑100 (MQ=255)

                                                        gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCa                                     <  1:1332067/100‑1 (MQ=255)

                                                        gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCCCCAGCTACAGtga                                     <  1:1618542/100‑3 (MQ=255)

                                                         tGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAg                                    >  2:1653959/1‑100 (MQ=255)

                                                          gAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAg                                    <  1:45171/99‑1 (MQ=255)

                                                          gAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGc                                   <  2:1645833/100‑1 (MQ=255)

                                                          gAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGc                                   <  1:258590/100‑1 (MQ=255)

                                                             tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                <  1:419167/100‑1 (MQ=255)

                                                              cAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACATTCAGCATcc                               >  2:575786/1‑100 (MQ=255)

                                                              cAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATcc                               <  1:484944/100‑1 (MQ=255)

                                                              cAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATcc                               >  2:483938/1‑100 (MQ=255)

                                                                 gCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAAc                            >  2:1792800/1‑100 (MQ=255)

                                                                    gTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACTGTCAGCATCCAACTTa                         <  2:1778377/100‑1 (MQ=255)

                                                                     tGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                        <  1:105441/100‑1 (MQ=255)

                                                                     tGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                        <  1:30051/100‑1 (MQ=255)

                                                                       gCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAg                      >  1:1358592/1‑100 (MQ=255)

                                                                          ggATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCt                   >  2:930517/1‑100 (MQ=255)

                                                                           gATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACCGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTg                  <  2:2114324/100‑1 (MQ=255)

                                                                              gggCTTCTCCATCCTGTGAGGGCTGAATGTCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg               <  2:449436/100‑1 (MQ=255)

                                                                               ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAGCTTACAGCCTGgtgt              >  1:2346755/1‑100 (MQ=255)

                                                                               ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt              >  1:1141213/1‑100 (MQ=255)

                                                                                gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg             <  1:930519/100‑1 (MQ=255)

                                                                                gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg             >  1:471278/1‑100 (MQ=255)

                                                                                gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg             >  1:471101/1‑100 (MQ=255)

                                                                                 cTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc            >  1:1076536/1‑100 (MQ=255)

                                                                                     tcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTc        >  2:2160582/1‑100 (MQ=255)

                                                                                      ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt       <  2:123873/100‑1 (MQ=255)

                                                                                      ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt       >  1:749356/1‑100 (MQ=255)

                                                                                      ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt       >  1:597315/1‑100 (MQ=255)

                                                                                      ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGGGTGCATTCt       >  2:333830/1‑100 (MQ=255)

                                                                                         tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc    <  2:1746501/100‑1 (MQ=255)

                                                                                         tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc    <  1:2160601/100‑1 (MQ=255)

                                                                                         tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc    >  1:1902673/1‑100 (MQ=255)

                                                                                           cTGTGAGGGCTTAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg  <  1:1871608/100‑1 (MQ=255)

                                                                                           cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACCGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg  >  2:1671448/1‑100 (MQ=255)

                                                                                           cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg  >  2:998323/1‑100 (MQ=255)

CCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGG  >  NC_000913/3542407‑3542597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A