Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F110 I0 R1 60 98.0 4713732 96.7% 4558178 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,542,503 G→A 59.3% W699* (TGG→TGA)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,5030GA59.3% 39.8 / 97.3 86W699* (TGG→TGA) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (14/21);  new base A (32/19);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 4.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGG  >  NC_000913/3542404‑3542597
                                                                                                   |                                                                                              
cTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgg                                                                                                <  1:1210696/100‑1 (MQ=255)
 tACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTggg                                                                                               <  1:266635/100‑1 (MQ=255)
  aCCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgggg                                                                                              >  1:1472220/1‑100 (MQ=255)
  aCCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgggg                                                                                              >  1:419561/1‑100 (MQ=255)
  aCCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgggg                                                                                              >  1:2283761/1‑100 (MQ=255)
   ccTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgaggg                                                                                             <  1:2320284/100‑1 (MQ=255)
     tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCt                                                                                           >  2:1688843/1‑100 (MQ=255)
     tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                           >  1:67847/1‑100 (MQ=255)
     tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                           >  2:2258015/1‑100 (MQ=255)
       aTTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGa                                                                                         <  1:2280085/100‑1 (MQ=255)
       aTTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGa                                                                                         <  1:2280017/100‑1 (MQ=255)
        ttttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGaa                                                                                        <  1:1609105/100‑1 (MQ=255)
         tttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAat                                                                                       >  1:1816017/1‑100 (MQ=255)
         tttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAat                                                                                       <  1:614589/100‑1 (MQ=255)
            cGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATc                                                                                    <  1:1625688/100‑1 (MQ=255)
                  gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                              <  1:1531172/100‑1 (MQ=255)
                  gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                              >  1:2124148/1‑100 (MQ=255)
                  gctgTATGTACCATGTATCTCGGGGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                              <  2:1474199/100‑1 (MQ=255)
                  gcggtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                              <  2:1474181/97‑1 (MQ=255)
                     gtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCa                                                                           >  1:511762/1‑100 (MQ=255)
                       atgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACt                                                                         >  1:619924/1‑100 (MQ=255)
                        tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTg                                                                        <  1:1699745/100‑1 (MQ=255)
                         gtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTgg                                                                       <  1:1935677/100‑1 (MQ=255)
                          taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGc                                                                      >  2:1108660/1‑100 (MQ=255)
                           aCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCa                                                                     <  1:1044161/100‑1 (MQ=255)
                           aCCATGTATCTCGGCGATGGGGGCTATCGCCCGTGAATCAAGCCGAGGCTGGATGGGCTTCACCATCCTGTGAGAGCTGAATATCGCTTACTCACTGGCa                                                                     >  2:145414/1‑100 (MQ=255)
                             cATGTATCTTGGTGATGGGGGCTATCGCCCGTGAGGCAAGCAGTGGCTGGAGGGGCTTCTCCATCCTGTGGGGGCTGAATCTCGCTTACTCACTggt                                                                      <  2:1524737/97‑2 (MQ=255)
                             cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   >  1:1738671/1‑100 (MQ=255)
                             cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   <  2:827207/100‑1 (MQ=255)
                              aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTTGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACa                                                                  <  2:2019464/100‑1 (MQ=255)
                              aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAAc                                                                   <  2:1415361/99‑1 (MQ=255)
                                  aTCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGt                                                              <  1:1006106/100‑1 (MQ=255)
                                  aTCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGt                                                              >  2:1100037/1‑100 (MQ=255)
                                   tctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGtt                                                             >  1:1136013/1‑100 (MQ=255)
                                   tctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGtt                                                             >  1:828844/1‑100 (MQ=255)
                                     tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTcn                                                           <  2:587718/100‑2 (MQ=255)
                                      cGGTGATGGGGGCTATCTCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGACTATCGCTTACTCACTAGCAACATTGttat                                                           >  2:1060507/1‑97 (MQ=255)
                                      cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTa                                                          >  2:683418/1‑100 (MQ=255)
                                       ggTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTAt                                                         >  2:2217745/1‑100 (MQ=255)
                                       ggTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTAt                                                         <  1:2222345/100‑1 (MQ=255)
                                        gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        >  1:2010482/1‑100 (MQ=255)
                                        gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        <  1:1261711/100‑1 (MQ=255)
                                        gTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                        >  2:632041/1‑100 (MQ=255)
                                         tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                       <  1:2029352/100‑1 (MQ=255)
                                          gaggggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGTTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAg                                                      <  1:638648/97‑1 (MQ=255)
                                          gATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGTGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAg                                                      >  1:813571/1‑100 (MQ=255)
                                          gATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAg                                                      >  2:2228043/1‑100 (MQ=255)
                                              ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  1:1463488/1‑100 (MQ=255)
                                              ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                  >  1:2077856/1‑100 (MQ=255)
                                               gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                 >  2:1095337/1‑100 (MQ=255)
                                                gggtcgcGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGcc                                                <  2:733833/94‑1 (MQ=255)
                                                 gctagggCCCGTGAATCAAGGCGTGGCGGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTAGcc                                                <  2:734750/93‑1 (MQ=255)
                                                  cTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTACTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAg                                              <  1:1656194/100‑1 (MQ=255)
                                                    aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCt                                            >  2:1218380/1‑100 (MQ=255)
                                                    aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCt                                            >  2:372389/1‑100 (MQ=255)
                                                        cccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAg                                        >  2:1644174/1‑100 (MQ=255)
                                                         ccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGt                                       <  1:2219478/100‑1 (MQ=255)
                                                          cGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTc                                      <  2:1572384/100‑1 (MQ=255)
                                                           gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCACCATTGTTCTAGCAGGTCGCCAGCTACAGTCa                                     >  2:2007702/1‑100 (MQ=255)
                                                              aaTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCa                                  <  1:1070719/100‑1 (MQ=255)
                                                               aTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCAt                                 >  1:784729/1‑100 (MQ=255)
                                                                tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                >  2:38466/1‑100 (MQ=255)
                                                                tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                >  1:78337/1‑100 (MQ=255)
                                                                tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                >  1:1236624/1‑100 (MQ=255)
                                                                   aGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCTTGTTTCTAGCTACAGTCAGCATCCa                              <  2:329691/99‑1 (MQ=255)
                                                                     ccGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACt                           >  1:290495/1‑100 (MQ=255)
                                                                      cGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACtt                          <  1:1734479/100‑1 (MQ=255)
                                                                       gTGGCTGGATGGGCGTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTa                         <  2:2186652/100‑1 (MQ=255)
                                                                        tGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                        <  2:511759/100‑1 (MQ=255)
                                                                          gCTGGATTGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                        <  2:2071781/98‑1 (MQ=255)
                                                                           cTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGc                     >  1:527724/1‑100 (MQ=255)
                                                                            tGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGcc                    >  1:2259790/1‑100 (MQ=255)
                                                                              gATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTg                  <  1:535118/100‑1 (MQ=255)
                                                                               aTGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTgg                 >  1:1026159/1‑100 (MQ=255)
                                                                                tGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgt                >  1:1263592/1‑100 (MQ=255)
                                                                                 gggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg               >  2:1464838/1‑100 (MQ=255)
                                                                                  ggCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt              <  1:1218385/100‑1 (MQ=255)
                                                                                  ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt              >  1:359260/1‑100 (MQ=255)
                                                                                  ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt              >  1:1832116/1‑100 (MQ=255)
                                                                                    cTTCTCCATCCTGTGGGGGCTGAATATCGCTTGCTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc            <  2:265777/100‑1 (MQ=255)
                                                                                    cTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc            <  2:290493/100‑1 (MQ=255)
                                                                                     ttCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCa           <  1:1259488/100‑1 (MQ=255)
                                                                                     ttCTCCATCCTGTGAGGGCGGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc            <  2:453260/99‑1 (MQ=255)
                                                                                      tctcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCAt          >  2:2123490/1‑100 (MQ=255)
                                                                                       ctcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCAtt         >  2:667183/1‑100 (MQ=255)
                                                                                        tcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTc        >  1:1417070/1‑100 (MQ=255)
                                                                                         ccATCCTGGGGGGGCTGAATATCGCTTACTCACGGGCAACATGGTTCTATGAGGTCGCCAGCTACAGTCACCATCCAACATACAGCCTGGTGGGAATTc        >  2:2049420/1‑99 (MQ=255)
                                                                                          cATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTg      >  1:936245/1‑100 (MQ=255)
                                                                                            tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc    <  1:86966/100‑1 (MQ=255)
                                                                                             ccTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCg   >  2:2206390/1‑100 (MQ=255)
                                                                                              cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg  >  1:95097/1‑100 (MQ=255)
                                                                                              cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg  <  2:1197721/100‑1 (MQ=255)
                                                                                                   |                                                                                              
CTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGG  >  NC_000913/3542404‑3542597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A