Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F183 I1 R1	63	136.1	6714777	94.6%	6352179	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,542,503	G→A	W699* (TGG→TGA)	feoB →	ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,542,503	0	G	A	96.3%	520.3 / 2.0	165	W699* (TGG→TGA)	feoB	ferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (78/80); total (82/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

ACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGT  >  NC_000913/3542406‑3542598

 ccTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgaggg                                                                                              >  1:2396523/1‑100 (MQ=255)

  cTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGc                                                                                             <  1:1293427/100‑1 (MQ=255)

  cTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGc                                                                                             <  1:1854275/100‑1 (MQ=255)

   tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGc                                                                                             <  2:480309/99‑1 (MQ=255)

   tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                            >  2:2241516/1‑100 (MQ=255)

    gATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTg                                                                                           <  2:2073676/100‑1 (MQ=255)

     aTTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGa                                                                                          <  1:1308750/100‑1 (MQ=255)

       tttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAat                                                                                        <  1:2772878/100‑1 (MQ=255)

         tCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAatat                                                                                      >  2:235879/1‑100 (MQ=255)

         tCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAatat                                                                                      >  2:235860/1‑100 (MQ=255)

           gTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCg                                                                                    >  1:589886/1‑100 (MQ=255)

            tCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGc                                                                                   <  1:1169437/100‑1 (MQ=255)

              ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCGAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCt                                                                                  <  2:1386448/99‑1 (MQ=255)

              ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCtt                                                                                 >  1:2953872/1‑100 (MQ=255)

              ctgctgTATGTACCATGTATCTCGGGGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATc                                                                                     <  2:3049268/96‑1 (MQ=255)

                gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                               >  2:3131901/1‑100 (MQ=255)

                gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                               >  1:1500867/1‑100 (MQ=255)

                gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                               <  1:327590/100‑1 (MQ=255)

                 ctgTATGTACCATGTATCTCGGGGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACt                                                                              <  2:1589603/100‑1 (MQ=255)

                  tgtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTc                                                                             >  2:911631/1‑100 (MQ=255)

                  tgtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTc                                                                             <  2:1219265/100‑1 (MQ=255)

                   gtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCa                                                                            >  1:697143/1‑100 (MQ=255)

                    tatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCAc                                                                           >  1:2598974/1‑100 (MQ=255)

                      tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTg                                                                         <  2:2131629/100‑1 (MQ=255)

                      tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACtn                                                                         <  2:3039526/100‑2 (MQ=255)

                      tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTg                                                                         <  2:1420005/100‑1 (MQ=255)

                       gtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTgg                                                                        >  2:1888414/1‑100 (MQ=255)

                        taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGc                                                                       <  2:273714/100‑1 (MQ=255)

                         aCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCa                                                                      >  2:1483239/1‑100 (MQ=255)

                          ccATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCaa                                                                     <  2:2623883/100‑1 (MQ=255)

                           cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                    >  2:1263409/1‑100 (MQ=255)

                           cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                    <  1:431166/100‑1 (MQ=255)

                             tGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACAt                                                                  >  2:396735/1‑100 (MQ=255)

                                aTCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGt                                                               <  2:3176608/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             <  1:230786/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             >  2:776774/1‑100 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             >  2:1088430/1‑100 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             <  2:1452113/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             <  1:1669134/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             <  1:1723028/100‑1 (MQ=255)

                                   tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCCTCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCt                                                            >  2:2944332/1‑100 (MQ=255)

                                    cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTa                                                           <  1:2508694/100‑1 (MQ=255)

                                    cGGGGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                             <  2:172569/98‑1 (MQ=255)

                                      ntGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                         >  1:2077941/2‑100 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                        <  1:902022/100‑1 (MQ=255)

                                          tGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTTACTGGCAACATTGTTCTATCAg                                                       <  2:1945776/98‑1 (MQ=255)

                                           gggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTc                                                    <  1:1811515/100‑1 (MQ=255)

                                           cttccgatctgCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTc                                                    <  2:777493/90‑1 (MQ=255)

                                            ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                   <  1:518743/100‑1 (MQ=255)

                                            ggggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                   >  1:2989076/1‑100 (MQ=255)

                                            ggggCGATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCg                                                   <  2:2695445/100‑1 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGATCgg                                                  >  2:2612071/1‑99 (MQ=255)

                                              ggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGcc                                                 <  2:1543530/100‑1 (MQ=255)

                                                 tATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGc                                              <  1:1483243/100‑1 (MQ=255)

                                                 tATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGc                                              >  1:2224405/1‑100 (MQ=255)

                                                  aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCt                                             >  2:2894597/1‑100 (MQ=255)

                                                    cGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTAc                                           <  1:2314468/100‑1 (MQ=255)

                                                        cGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAggc                                       >  2:3037513/1‑98 (MQ=255)

                                                        cGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTc                                       >  2:3004598/1‑100 (MQ=255)

                                                         gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCa                                      >  1:1597793/1‑100 (MQ=255)

                                                          tGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAg                                     <  1:2699654/100‑1 (MQ=255)

                                                           gAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGc                                    <  2:2224394/100‑1 (MQ=255)

                                                            aaTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGc                                    <  2:2914623/99‑1 (MQ=255)

                                                             aTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCAt                                  >  1:1197366/1‑100 (MQ=255)

                                                             aTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCAt                                  >  1:1461258/1‑100 (MQ=255)

                                                               cAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATcc                                >  1:565340/1‑100 (MQ=255)

                                                               cAAGCCGTGGCTGGATGGGCTGCTCCATCCTGTGAGGGCTGAATATCGCGTACTCACTGGCAACATTGATCTATCAGGTCGCCAGCCACAGTCAGCATcc                                >  2:2606823/1‑100 (MQ=255)

                                                                 aGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCaa                              >  1:2379670/1‑100 (MQ=255)

                                                                 aGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCaa                              >  2:1373545/1‑100 (MQ=255)

                                                                  gCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAAc                             <  2:2518496/100‑1 (MQ=255)

                                                                  gCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAAc                             <  2:2460700/100‑1 (MQ=255)

                                                                   ccGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACt                            >  2:1837692/1‑100 (MQ=255)

                                                                   ccGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACt                            <  1:1038238/100‑1 (MQ=255)

                                                                     gTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTa                          >  1:1974936/1‑100 (MQ=255)

                                                                      tGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                         <  1:2545230/100‑1 (MQ=255)

                                                                      tGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAc                         >  2:1431747/1‑100 (MQ=255)

                                                                       ggCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACa                        >  1:26100/1‑100 (MQ=255)

                                                                         cTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGc                      <  1:2492830/100‑1 (MQ=255)

                                                                           ggATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCt                    >  2:652780/1‑100 (MQ=255)

                                                                           ggATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCt                    <  1:2332844/100‑1 (MQ=255)

                                                                           ggATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCt                    <  1:939127/100‑1 (MQ=255)

                                                                                ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt               >  2:1078052/1‑100 (MQ=255)

                                                                                 gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg              >  2:1704026/1‑100 (MQ=255)

                                                                                 gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg              <  2:3259959/100‑1 (MQ=255)

                                                                                  cTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc             <  1:249889/100‑1 (MQ=255)

                                                                                  cTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGc             >  1:348082/1‑100 (MQ=255)

                                                                                    tctcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCAt           >  2:198026/1‑100 (MQ=255)

                                                                                    tctcCATCCTGTGAGGGCTGAATATCGCTTACTCACTAGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCAt           >  2:1793785/1‑100 (MQ=255)

                                                                                      tcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTc         >  1:309570/1‑100 (MQ=255)

                                                                                       ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt        >  1:607801/1‑100 (MQ=255)

                                                                                       ccATCCTGTGAGGGCAGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt        >  1:2987916/1‑100 (MQ=255)

                                                                                        cATCCTGTGAGGGCTGAATATCGCTTACTCATTGGCATCATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTg       <  2:2260241/100‑1 (MQ=255)

                                                                                        cATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTg       >  2:2483298/1‑100 (MQ=255)

                                                                                        cATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTg       >  1:1892290/1‑100 (MQ=255)

                                                                                          tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc     <  1:396736/100‑1 (MQ=255)

                                                                                          tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc     >  1:2586566/1‑100 (MQ=255)

                                                                                          tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc     >  2:811646/1‑100 (MQ=255)

                                                                                            ntgtgAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg   >  2:2482905/2‑100 (MQ=255)

                                                                                            cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg   >  1:2176429/1‑100 (MQ=255)

                                                                                             tgtgAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGt  <  1:811648/100‑1 (MQ=255)

ACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGT  >  NC_000913/3542406‑3542598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A