Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F232 I0 R1	243	156.3	7646460	93.8%	7172379	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,542,503	G→A	100%	W699* (TGG→TGA)	feoB →	ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,542,503	0	G	A	97.7%	594.7 / ‑0.4	186	W699* (TGG→TGA)	feoB	ferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (99/80); total (103/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 190 total aligned reads displayed.

ACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTT  >  NC_000913/3542406‑3542599

aCCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgagg                                                                                                <  1:3006163/100‑1 (MQ=255)

 ccTGGTTTTCGTCCTGCTGTATGCACTATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTgagg                                                                                                <  2:896157/99‑1 (MQ=255)

  cTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGc                                                                                              >  1:2185582/1‑100 (MQ=255)

   tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                             >  1:3488422/1‑100 (MQ=255)

   tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                             >  1:1647781/1‑100 (MQ=255)

   tGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCAGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCt                                                                                             >  2:1735295/1‑100 (MQ=255)

    gATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTg                                                                                            <  1:381722/100‑1 (MQ=255)

       tttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAat                                                                                         <  1:868488/100‑1 (MQ=255)

        ttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAata                                                                                        <  1:665134/100‑1 (MQ=255)

        ttCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAata                                                                                        <  2:1917090/100‑1 (MQ=255)

        ntCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGCGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAata                                                                                        >  2:1129052/2‑100 (MQ=255)

         tCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAatat                                                                                       >  2:1563077/1‑100 (MQ=255)

          cGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATc                                                                                      >  2:655491/1‑100 (MQ=255)

             ccTGCTGTATGTACCATGTATCTCGGTGAGCGGGGCAATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGc                                                                                    <  2:2567082/99‑1 (MQ=255)

              ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCtt                                                                                  >  1:155393/1‑100 (MQ=255)

              ctgctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCt                                                                                   <  2:415929/99‑1 (MQ=255)

                gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                                <  1:1991431/100‑1 (MQ=255)

                gctgTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                                >  1:102657/1‑100 (MQ=255)

                  tgtttgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTAc                                                                                <  2:536451/98‑1 (MQ=255)

                  tgtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTc                                                                              >  1:3361574/1‑100 (MQ=255)

                   gtatgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCa                                                                             >  1:85920/1‑100 (MQ=255)

                     atgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACt                                                                           >  1:1847914/1‑100 (MQ=255)

                      tgtaCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTg                                                                          <  2:2972270/100‑1 (MQ=255)

                       gtgccAGGTATCTGGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTgg                                                                         <  2:1255515/97‑1 (MQ=255)

                        taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGc                                                                        <  1:2541561/100‑1 (MQ=255)

                        taCCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGc                                                                        >  2:3704649/1‑100 (MQ=255)

                          ccATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCaa                                                                      >  2:335882/1‑100 (MQ=255)

                          ccATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCaa                                                                      >  1:3345372/1‑100 (MQ=255)

                           cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                     <  1:2264415/100‑1 (MQ=255)

                           cATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAAc                                                                     >  1:3590298/1‑100 (MQ=255)

                           cATGTATCTCGGTGATGGGCGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGGGAGAGCTGAATATCGCTTCCTCACTGGc                                                                        >  2:735108/1‑97 (MQ=255)

                            aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACa                                                                    <  1:3326292/100‑1 (MQ=255)

                            aTGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACa                                                                    <  1:818588/100‑1 (MQ=255)

                             ngTATCTCGGTGATGGGGGCTATAGCCCGTGAATCAAGCCGAGGCTGGATGAGCTTCTCAAACATGTCAGGGCTGAATCTCGCTTCCTCACTCGc                                                                        >  2:2080088/2‑95 (MQ=255)

                                 tctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGtt                                                               >  2:2060652/1‑100 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGATGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                              <  2:3139287/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                              <  1:3123689/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                              <  1:981951/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                              <  1:2808643/100‑1 (MQ=255)

                                  ctcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTc                                                              >  1:3643686/1‑100 (MQ=255)

                                   tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCt                                                             >  1:3183127/1‑100 (MQ=255)

                                   tcGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCt                                                             >  1:1354671/1‑100 (MQ=255)

                                    cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTa                                                            >  1:2243520/1‑100 (MQ=255)

                                    cGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTa                                                            >  1:100621/1‑100 (MQ=255)

                                    cGGTGATGGGGGCTATCGCCCGTGAATCAAACCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTa                                                            >  1:2366612/1‑100 (MQ=255)

                                    cGGTGACGGGGTCTATCGCCCGTCGATCAAGCCGTGGCTGGATAGGCTCCGCCATCCTGTGAGGTCTGAATATCGCTTACTCACTGGCAACCTTGTTCt                                                             <  2:2621757/99‑1 (MQ=255)

                                      gTGATGGGGGCTATCGCCCGTGAATGAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGCGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATc                                                          <  2:2221751/100‑1 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                         <  1:2697323/100‑1 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                         <  1:1449754/100‑1 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                         >  1:3002140/1‑100 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                         <  1:1806616/100‑1 (MQ=255)

                                       tGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCa                                                         <  2:3160324/100‑1 (MQ=255)

                                          tGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGt                                                      >  2:246312/1‑100 (MQ=255)

                                          cacgggCGATAGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAgg                                                       >  1:3540326/4‑99 (MQ=255)

                                            ggggCTATCGCCCATGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACCGGCCACATTGTTCTATCAGGTc                                                     >  2:2365788/1‑99 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                   >  1:2490714/1‑100 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                   >  2:2427805/1‑100 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                   >  2:624836/1‑100 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                   >  2:1835293/1‑100 (MQ=255)

                                             gggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGc                                                   >  2:1990285/1‑100 (MQ=255)

                                              ggCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGcc                                                  >  1:164875/1‑100 (MQ=255)

                                                 tATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGc                                               <  1:827895/100‑1 (MQ=255)

                                                  aTCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCt                                              <  1:488750/100‑1 (MQ=255)

                                                     gCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACa                                           <  2:2791256/100‑1 (MQ=255)

                                                      cccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAg                                          >  1:2157868/1‑100 (MQ=255)

                                                       ccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGt                                         >  2:2926786/1‑100 (MQ=255)

                                                       ccGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGt                                         >  2:504463/1‑100 (MQ=255)

                                                         gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTTTGAAGGCTGAATATCGCTTACGCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAg                                          <  2:3610871/97‑1 (MQ=255)

                                                         gTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCa                                       <  1:2129631/100‑1 (MQ=255)

                                                            aaTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCa                                    <  1:69753/100‑1 (MQ=255)

                                                             aTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGGCGCCAGCTACAGTCAGCAt                                   >  1:2293174/1‑100 (MQ=255)

                                                             aTCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCAt                                   >  2:715582/1‑100 (MQ=255)

                                                              tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                  >  1:928064/1‑100 (MQ=255)

                                                              tCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATc                                  >  1:3431906/1‑100 (MQ=255)

                                                                  gCCGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAAc                              >  1:1629090/1‑100 (MQ=255)

                                                                   ccGTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACt                             <  1:100126/100‑1 (MQ=255)

                                                                     gTGGCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTa                           >  1:611441/1‑100 (MQ=255)

                                                                       ggCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTAcn                         <  2:3361548/100‑2 (MQ=255)

                                                                        gCTGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACat                        <  2:345797/100‑2 (MQ=255)

                                                                          tGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGcc                      >  2:480058/1‑100 (MQ=255)

                                                                          tGGATGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGc                       <  2:1181838/99‑1 (MQ=255)

                                                                             aTGGGCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTgg                   >  1:1598533/1‑100 (MQ=255)

                                                                               gggCTTCTCCATCCTGTGACGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg                 <  2:3237717/100‑1 (MQ=255)

                                                                               gatcTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg                 <  2:813158/97‑1 (MQ=255)

                                                                               gatcTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg                 <  2:2432555/97‑1 (MQ=255)

                                                                                ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtgt                >  1:2610552/1‑100 (MQ=255)

                                                                                ggCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGgtg                 >  2:2156939/1‑99 (MQ=255)

                                                                                 gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg               >  2:3567323/1‑100 (MQ=255)

                                                                                 gCTTCTCCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGtgtg               >  1:1726445/1‑100 (MQ=255)

                                                                                    tctcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCAt            <  1:1503770/100‑1 (MQ=255)

                                                                                    tctcCACCATGTGGGGGCTGGGTATCTCTTACTCACTGGCAACAATGTTCTTTCAGGTGGCCAGCTATAGTCAGCATCCAACTTACTGCCTGGTGTGCa             <  2:799071/99‑1 (MQ=255)

                                                                                      tcCATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTc          <  2:3442851/100‑1 (MQ=255)

                                                                                       ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt         >  2:1112734/1‑100 (MQ=255)

                                                                                       ccATCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCt         >  1:2923098/1‑100 (MQ=255)

                                                                                         aTCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTgg       <  2:560708/100‑1 (MQ=255)

                                                                                         aTCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTgg       >  1:1571087/1‑100 (MQ=255)

                                                                                          tCCTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGc      <  2:3488395/100‑1 (MQ=255)

                                                                                           ccTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCg     >  1:20629/1‑100 (MQ=255)

                                                                                            cTGTGAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCgg    >  1:3618263/1‑100 (MQ=255)

                                                                                              gtgAGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGtt  >  2:1437873/1‑100 (MQ=255)

ACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTATCGCCCGTGAATCAAGCCGTGGCTGGATGGGCTTCTCCATCCTGTGGGGGCTGAATATCGCTTACTCACTGGCAACATTGTTCTATCAGGTCGCCAGCTACAGTCAGCATCCAACTTACAGCCTGGTGTGCATTCTGGCGGTT  >  NC_000913/3542406‑3542599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A