Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,542,093 G→A 37.7% V563M (GTG→ATG)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,0930GA37.7% 131.7 / 150.6 143V563M (GTG→ATG) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/53);  new base A (27/27);  total (63/80)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 144 total aligned reads displayed.

ACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAA  >  NC_000913/3541995‑3542192
                                                                                                  |                                                                                                   
aCAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGt                                                                                                    <  1:1085217/100‑1 (MQ=255)
 cAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGCCTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTg                                                                                                   <  1:799263/100‑1 (MQ=255)
 cAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTg                                                                                                   <  1:2883706/100‑1 (MQ=255)
   gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa                                                                                                 >  1:1983697/1‑100 (MQ=255)
   gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa                                                                                                 >  1:1738177/1‑100 (MQ=255)
      tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                              >  1:2286529/1‑100 (MQ=255)
       ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCTGCGCTGGCGTCCGTCAGCCGGCTGCTCCCCCCGGTCTTTAACCCAATTGGCATGc                                                                                                  >  1:2579268/1‑95 (MQ=255)
       ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                                                             <  2:3086834/100‑1 (MQ=255)
       ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGaa                                                                                             <  1:3324604/100‑1 (MQ=255)
        tcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAg                                                                                            <  2:1347932/100‑1 (MQ=255)
         cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGa                                                                                           >  2:2952062/1‑100 (MQ=255)
          gCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGAt                                                                                          >  2:1386202/1‑100 (MQ=255)
           cTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATa                                                                                         >  2:2211867/1‑100 (MQ=255)
              aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                                                      >  1:348193/1‑100 (MQ=255)
               gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTg                                                                                     <  2:2586094/100‑1 (MQ=255)
               gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg                                                                                     >  2:1628906/1‑100 (MQ=255)
                 gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                                                   >  1:1423262/1‑100 (MQ=255)
                 gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                                                   <  2:3215735/100‑1 (MQ=255)
                 gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCACGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggc                                                                                   >  2:2917550/1‑100 (MQ=255)
                 gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCCTCCGTCAGCCGGGTGATCAGCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                                                   <  1:1886260/100‑1 (MQ=255)
                  ggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                                                                  >  2:497945/1‑100 (MQ=255)
                   gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcag                                                                                 <  1:1035551/100‑1 (MQ=255)
                   gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCCTCAAGCCAATTGGCGTGCATGAAGATAACTggcag                                                                                 >  2:1021087/1‑100 (MQ=255)
                     aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggc                                                                               <  2:454324/100‑1 (MQ=255)
                     aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggc                                                                               <  2:454261/100‑1 (MQ=255)
                     aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcaggc                                                                               >  2:1744695/1‑100 (MQ=255)
                     aaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcaggc                                                                               <  1:3071203/100‑1 (MQ=255)
                           tcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                                                         >  2:1391861/1‑100 (MQ=255)
                             gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                                                       >  2:1852095/1‑100 (MQ=255)
                             gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGCTg                                                                       <  2:558227/100‑1 (MQ=255)
                              aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                      <  1:3368729/100‑1 (MQ=255)
                               tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                     >  1:2545096/1‑100 (MQ=255)
                                aaCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                    <  2:2295842/100‑1 (MQ=255)
                                aaCATCAACGACTCGGCGCTGGCGTCAGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                    >  2:2102956/1‑100 (MQ=255)
                                 aCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCt                                                                   >  2:1354561/1‑100 (MQ=255)
                                 aCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCt                                                                   >  1:843277/1‑100 (MQ=255)
                                 aCATCAACGACTCGGCGCTGGCGCCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAGTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                    <  1:2238212/99‑1 (MQ=255)
                                  cATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                                                                  >  2:1501148/1‑100 (MQ=255)
                                   aTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                                                                 <  2:6302/100‑1 (MQ=255)
                                   aTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                                                                 >  1:3197800/1‑100 (MQ=255)
                                   aTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGt                                                                 >  2:1994615/1‑100 (MQ=255)
                                    tCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                                                                  >  1:2588989/1‑98 (MQ=255)
                                     cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                                                               <  1:1513743/100‑1 (MQ=255)
                                      aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                              >  2:814827/1‑100 (MQ=255)
                                      aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                              >  1:1446473/1‑100 (MQ=255)
                                       aCGACTCGGCGCTGGTGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                                                             <  2:1530007/100‑1 (MQ=255)
                                       aCGACTCGGCGCTCGAGTCCGTCAGAAAGGTGATCACACCGGTATACAAGACAAGTGGCATGCATAAAGATAACTGGCAGGCAACGGTTGGCCTGCTTAc                                                             <  1:2885949/100‑1 (MQ=255)
                                         gACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAg                                                           <  2:2789691/100‑1 (MQ=255)
                                         gACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAg                                                           <  2:2779815/100‑1 (MQ=255)
                                         gACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAg                                                           >  2:2754085/1‑100 (MQ=255)
                                          aCTCGGCGCTGGCGTCCGTCAGCCGGGTGGTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg                                                          <  1:2088659/100‑1 (MQ=255)
                                          aCTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg                                                          <  2:2826492/100‑1 (MQ=255)
                                            tCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                                                        <  1:2049633/100‑1 (MQ=255)
                                             cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                                                       <  2:841618/100‑1 (MQ=255)
                                             cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                                                       <  1:2280056/100‑1 (MQ=255)
                                               gcgcTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                                                     <  2:2073467/100‑1 (MQ=255)
                                                cgcTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                                                     >  1:1724320/1‑99 (MQ=255)
                                                  cTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                                                  <  2:1423274/100‑1 (MQ=255)
                                                  cTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                                                  >  2:2146306/1‑100 (MQ=255)
                                                  cTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                                                  <  2:429655/100‑1 (MQ=255)
                                                    ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                                <  1:1628890/100‑1 (MQ=255)
                                                         ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga                                           >  2:1997787/1‑100 (MQ=255)
                                                          cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa                                          >  2:2472534/1‑100 (MQ=255)
                                                           gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                         <  2:887123/100‑1 (MQ=255)
                                                           gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                         <  2:1241545/100‑1 (MQ=255)
                                                            tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt                                        <  2:1143676/100‑1 (MQ=255)
                                                             cAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                       >  2:1813592/1‑100 (MQ=255)
                                                               gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt                                     >  1:254262/1‑100 (MQ=255)
                                                               gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt                                     >  1:641989/1‑100 (MQ=255)
                                                               gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt                                     <  2:947907/100‑1 (MQ=255)
                                                                  gggTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGt                                  >  1:2583457/1‑100 (MQ=255)
                                                                     tGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg                               <  1:681080/100‑1 (MQ=255)
                                                                      gATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCCACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt                              >  1:114290/1‑100 (MQ=255)
                                                                       aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                             <  2:1526656/100‑1 (MQ=255)
                                                                          aCCCCGGCCTTCAAGCCAATTGGCGTGCATGAAGAAAACTGGCCGGCAACGGTTGGACCGTTGACAGGTGCCATGGCGAAAGAAGTGGGAGtacgcacg                           >  1:3151653/1‑92 (MQ=255)
                                                                           ccccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                         >  1:41314/1‑100 (MQ=255)
                                                                           ccccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                         <  2:3248962/100‑1 (MQ=255)
                                                                           ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                         >  1:1503570/1‑100 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                       <  1:3031430/100‑1 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                       <  1:369400/100‑1 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                       <  1:1503855/100‑1 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGATTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGCGGTAGTGGGTACGCTCa                       <  1:2063142/100‑1 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                       <  2:882796/100‑1 (MQ=255)
                                                                             ccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                       <  2:1705070/100‑1 (MQ=255)
                                                                               ggTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                     <  2:1091305/100‑1 (MQ=255)
                                                                                gTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAaca                    <  2:2592075/100‑1 (MQ=255)
                                                                                gTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAaca                    <  1:1689304/100‑1 (MQ=255)
                                                                                  cTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTCCCGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAacaac                  >  1:3430505/1‑98 (MQ=255)
                                                                                   ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc                 <  2:2545115/100‑1 (MQ=255)
                                                                                    tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                >  2:1471360/1‑100 (MQ=255)
                                                                                    tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                >  2:948673/1‑100 (MQ=255)
                                                                                    tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                <  1:1165203/100‑1 (MQ=255)
                                                                                    tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                <  2:174052/100‑1 (MQ=255)
                                                                                     cAAGCCAATTGGCATGGATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc               <  1:2023009/100‑1 (MQ=255)
                                                                                     cAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc               <  1:3016153/100‑1 (MQ=255)
                                                                                          cAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac          >  1:705001/1‑100 (MQ=255)
                                                                                           aaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc         <  2:41314/100‑1 (MQ=255)
                                                                                            aTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg        <  2:1962835/100‑1 (MQ=255)
                                                                                            aTTGGCGTGCATGAAGATAACTGGCACGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGCAGTGGTCGTGGGTACGCTCCACACCCTCTccac          <  1:1023216/98‑1 (MQ=255)
                                                                                                  gTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaaa  <  2:1733811/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
ACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAA  >  NC_000913/3541995‑3542192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A