Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F255 I0 R1 121 91.8 4500463 95.1% 4279940 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,542,093 G→A 31.4% V563M (GTG→ATG)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,0930GA31.4% 111.1 / 63.2 84V563M (GTG→ATG) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/34);  new base A (11/15);  total (34/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGA  >  NC_000913/3541994‑3542190
                                                                                                   |                                                                                                 
aaCAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                   <  1:1655042/100‑1 (MQ=255)
 aCAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGt                                                                                                  >  1:1972618/1‑100 (MQ=255)
  cAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCTCCCCGGTCTTAAAGCCAATTGGCGTg                                                                                                 <  2:780359/100‑1 (MQ=255)
   aGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                                                <  1:1434185/100‑1 (MQ=255)
     cTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                                                              <  1:955255/100‑1 (MQ=255)
     cTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                                                              >  2:257448/1‑100 (MQ=255)
     cTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa                                                                                               <  2:1099334/99‑1 (MQ=255)
     cTTCGCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCATCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATg                                                                                                 <  2:1202843/97‑1 (MQ=255)
      ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                                                             >  2:1058391/1‑100 (MQ=255)
         tcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                                                          <  2:2026398/100‑1 (MQ=255)
          cgttGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGa                                                                                         <  1:1829624/97‑1 (MQ=255)
          cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGAGTGATCACCCCGGTCTTCAAGCCAATTCGCGTGCATGAAGa                                                                                         >  2:1293655/1‑100 (MQ=255)
            cTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATa                                                                                       >  1:1945552/1‑100 (MQ=255)
                gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                                    >  1:595632/1‑99 (MQ=255)
                gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                                    >  2:895686/1‑99 (MQ=255)
                gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg                                                                                   >  2:1619998/1‑100 (MQ=255)
                gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCGGGCGTCCGTCGTCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTg                                                                                   >  2:743918/1‑100 (MQ=255)
                  gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCCCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                                                 >  1:1031772/1‑100 (MQ=255)
                  gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATaaat                                                                                    >  1:147068/1‑95 (MQ=255)
                  gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCA‑CCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggca                                                                                <  2:1576380/100‑1 (MQ=255)
                    gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcag                                                                               >  2:428740/1‑100 (MQ=255)
                     aaaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                                                              <  1:1160283/100‑1 (MQ=255)
                            tcgATAACATCAACGACTCGGCGCTGGAGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                                                       <  2:705581/100‑1 (MQ=255)
                             cgATAACATCAACTATTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGtt                                                                      <  1:187230/100‑1 (MQ=255)
                              gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGTCAGGCAACGGtcg                                                                     <  1:1521751/100‑3 (MQ=255)
                              gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTg                                                                     <  2:542775/100‑1 (MQ=255)
                               agaaCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                    >  1:1516043/3‑100 (MQ=255)
                               aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                    <  1:1438846/100‑1 (MQ=255)
                                tAACATCAACGGGTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAACTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                   <  2:1192509/100‑1 (MQ=255)
                                tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                   <  2:201514/100‑1 (MQ=255)
                                 aaCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                  <  1:1295769/100‑1 (MQ=255)
                                 aaCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                  >  1:1643108/1‑100 (MQ=255)
                                   cATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATAACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCt                                                                 >  2:2061602/1‑99 (MQ=255)
                                      cAACGACGCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCCTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                                                             <  2:1382579/100‑1 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                            >  2:1440238/1‑100 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                                                             <  2:1783927/99‑1 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                            <  1:301166/100‑1 (MQ=255)
                                        aCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                                                           <  1:429413/100‑1 (MQ=255)
                                        aCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                                                           >  2:415388/1‑100 (MQ=255)
                                         cGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACa                                                          <  2:1661963/100‑1 (MQ=255)
                                            cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                                                       >  2:153367/1‑100 (MQ=255)
                                            cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACATGt                                                       >  1:458336/1‑100 (MQ=255)
                                               ggCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                    <  2:919134/100‑1 (MQ=255)
                                                    tGGCGTCCGTCAGTCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                                                <  1:615321/99‑1 (MQ=255)
                                                    tGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc                                               <  1:1058393/100‑1 (MQ=255)
                                                    tGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc                                               <  2:1182541/100‑1 (MQ=255)
                                                       cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                            >  1:1283479/1‑100 (MQ=255)
                                                       cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGCTTGACCTGTTTACAGGTGCCATGGCGaa                                            >  2:2087148/1‑100 (MQ=255)
                                                        gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa                                           <  2:940995/100‑1 (MQ=255)
                                                         tccgtcAGCAGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACAGTTGGCCTGTTTACAGGTGCCATGGCGAaag                                          <  2:1259858/100‑1 (MQ=255)
                                                          ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaaaa                                         >  1:1770889/1‑98 (MQ=255)
                                                           cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa                                        >  2:1312302/1‑100 (MQ=255)
                                                            gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAGGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                       >  2:1074647/1‑100 (MQ=255)
                                                              cAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                     <  1:563928/100‑1 (MQ=255)
                                                               aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGCTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg                                    <  1:1579081/100‑1 (MQ=255)
                                                                     tttatcatcCCTGTCTTCAATCCAATTGTCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                               <  1:43566/96‑1 (MQ=255)
                                                                     gTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                               <  1:1117825/99‑1 (MQ=255)
                                                                     gTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTgg                              <  1:1259588/100‑1 (MQ=255)
                                                                      tGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg                             >  1:1669665/1‑100 (MQ=255)
                                                                      tGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg                             >  2:26748/1‑100 (MQ=255)
                                                                       gATCACCCCGGTCTTCAAGCCAATTGGCGTCCATGAAGATACCTGGCAGTCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGt                                <  2:962045/96‑1 (MQ=255)
                                                                        aTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                           <  2:1606210/100‑1 (MQ=255)
                                                                         tCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTAc                          <  2:670524/100‑1 (MQ=255)
                                                                          cACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACg                         >  2:1477098/1‑100 (MQ=255)
                                                                           aCCCCGGTCTTCAAGCCAATTGGCGTGCATGAAAATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGTCATGGCGAAAGAAGTGGTAGTGGGTACGc                        >  2:1349417/1‑100 (MQ=255)
                                                                              ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                     <  1:1312308/100‑1 (MQ=255)
                                                                              ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                     <  2:1283473/100‑1 (MQ=255)
                                                                              ccGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                     <  2:2249465/100‑1 (MQ=255)
                                                                                ggTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                   <  1:740982/100‑1 (MQ=255)
                                                                                ggTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                   <  1:2028078/100‑1 (MQ=255)
                                                                                    ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc               <  1:596470/100‑1 (MQ=255)
                                                                                     tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc               <  1:1487779/99‑1 (MQ=255)
                                                                                     tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct              <  1:873938/100‑1 (MQ=255)
                                                                                      cAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc             <  1:1525588/100‑1 (MQ=255)
                                                                                      cAAGCCAATTGGCGTGCATGAAGAGAACTGGCAGGCACCGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc             <  2:1514264/100‑1 (MQ=255)
                                                                                       aaGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct            >  1:1137522/1‑100 (MQ=255)
                                                                                        aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa           >  2:1373188/1‑100 (MQ=255)
                                                                                        aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa           >  1:1164758/1‑100 (MQ=255)
                                                                                        aGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGACGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTa           >  2:1506801/1‑100 (MQ=255)
                                                                                         gtcAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTac          <  1:984806/98‑1 (MQ=255)
                                                                                           cAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac        <  2:1421880/100‑1 (MQ=255)
                                                                                              ttGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGc     <  1:428739/100‑1 (MQ=255)
                                                                                               tGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCa    <  1:1705369/100‑1 (MQ=255)
                                                                                                ggCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAg   >  1:1237939/1‑100 (MQ=255)
                                                                                                 gCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa  >  1:194955/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
AACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGA  >  NC_000913/3541994‑3542190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A