Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,542,093 G→A 100% V563M (GTG→ATG)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,0930GA97.7% 277.4 / ‑3.7 86V563M (GTG→ATG) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (39/45);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

TTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACC  >  NC_000913/3542000‑3542185
                                                                                             |                                                                                            
ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGcatgcatg                                                                                        <  2:220700/100‑1 (MQ=255)
 tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGa                                                                                       <  1:77342/100‑1 (MQ=255)
  ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGcatgcatg                                                                                        <  1:501510/98‑1 (MQ=255)
    cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTAATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGa                                                                                    <  2:2086448/100‑1 (MQ=255)
     gCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGAt                                                                                   <  2:101534/100‑1 (MQ=255)
      cTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATa                                                                                  <  2:2132247/100‑1 (MQ=255)
      cTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGCCAGCCGGGTGACCACCCCGGTCTTCAAGCCAAACGGCAGGCATGCAGATa                                                                                  >  1:853872/1‑100 (MQ=255)
         aGCGGTAAAATCGTCGATAACATCAACGACTCGGCGCTGACGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                               <  1:2260373/100‑1 (MQ=255)
         aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                               <  1:1023268/100‑1 (MQ=255)
            gtggaaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggc                                                                            <  1:749919/96‑1 (MQ=255)
             ggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggca                                                                           >  1:1154113/1‑100 (MQ=255)
             ggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggca                                                                           >  1:1307534/1‑100 (MQ=255)
              gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcag                                                                          <  1:390128/100‑1 (MQ=255)
                    cgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACg                                                                    >  2:2534956/1‑100 (MQ=255)
                     gtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACgg                                                                   >  2:1881264/1‑100 (MQ=255)
                     gtcgATAACATCAACGACGCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACgg                                                                   <  2:1120804/100‑1 (MQ=255)
                      tcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGt                                                                  <  2:868099/100‑1 (MQ=255)
                      tcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGt                                                                  >  1:161634/1‑100 (MQ=255)
                      tcgATAACATCAACGACTCGGCGCTGGCGTACGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCCTGAAGATAACTGGCAGGCAACgg                                                                   >  1:530031/1‑99 (MQ=255)
                        gATAACATCAACGACTCGGCGCTGGCTTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGATTg                                                                <  1:2537173/100‑1 (MQ=255)
                         aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg                                                               >  2:524658/1‑100 (MQ=255)
                         aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg                                                               <  2:804053/100‑1 (MQ=255)
                          tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                              <  1:2568451/100‑1 (MQ=255)
                          tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCACGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                              <  2:1411225/100‑1 (MQ=255)
                            aCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCt                                                            >  2:47207/1‑100 (MQ=255)
                               tCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGtt                                                         >  2:2377468/1‑100 (MQ=255)
                                 aaCGACTCGGCGCTGGCATCCGTCAGCCGGGTGATCACCCCGGGGTTCAAGCCAATTGGCAGGCATGAAGATAACTGGCAGGCAACGGTTTGCCTGCTTa                                                       >  1:2086108/1‑100 (MQ=255)
                                  aCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                                                      >  1:2008843/1‑100 (MQ=255)
                                      ntCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                                                  >  1:553152/2‑100 (MQ=255)
                                      cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                                                  >  2:2421575/1‑100 (MQ=255)
                                      cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGt                                                  >  1:930550/1‑100 (MQ=255)
                                       tCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                                                 <  2:1474678/100‑1 (MQ=255)
                                        cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                                                 <  2:1148596/99‑1 (MQ=255)
                                           cgcTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAt                                             <  1:1921822/100‑1 (MQ=255)
                                           cgcTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAt                                             >  1:1612695/1‑100 (MQ=255)
                                           cgcTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCATGTTTACAGGTGCCAt                                             >  1:1514271/1‑100 (MQ=255)
                                           cgcTGGCGCCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCATGTTTACAGGTGCCAt                                             >  1:1514583/1‑100 (MQ=255)
                                             cTGGCGGCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                                           <  2:975571/100‑1 (MQ=255)
                                              tGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc                                          <  2:1563694/100‑1 (MQ=255)
                                              tGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc                                          <  2:2456273/100‑1 (MQ=255)
                                               ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                         >  1:521541/1‑100 (MQ=255)
                                               ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                         <  2:975326/100‑1 (MQ=255)
                                               ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                         <  2:2225982/100‑1 (MQ=255)
                                               ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                         <  2:2376546/100‑1 (MQ=255)
                                               ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                         >  2:612782/1‑100 (MQ=255)
                                                 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                       >  2:634988/1‑100 (MQ=255)
                                                 cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                       >  2:634948/1‑100 (MQ=255)
                                                  gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa                                      <  2:832162/100‑1 (MQ=255)
                                                  gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa                                      <  1:2322091/100‑1 (MQ=255)
                                                    ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga                                    <  1:1318827/100‑1 (MQ=255)
                                                     cgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGCaagaa                                   >  2:975916/1‑100 (MQ=255)
                                                      gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                  <  1:832832/100‑1 (MQ=255)
                                                       tcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt                                 <  1:1754071/100‑1 (MQ=255)
                                                        cAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                <  1:122769/100‑1 (MQ=255)
                                                        cAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                <  1:2435493/100‑1 (MQ=255)
                                                         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg                               <  2:2245722/100‑1 (MQ=255)
                                                         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg                               >  2:372657/1‑100 (MQ=255)
                                                         aGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTgg                               <  1:2421560/100‑1 (MQ=255)
                                                          gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt                              <  2:371121/100‑1 (MQ=255)
                                                          gCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGt                              >  2:151747/1‑100 (MQ=255)
                                                            cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                            >  2:1071442/1‑100 (MQ=255)
                                                             gggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGt                           >  2:886204/1‑100 (MQ=255)
                                                              ggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                          <  1:2429486/100‑1 (MQ=255)
                                                              ggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                          <  2:774183/100‑1 (MQ=255)
                                                                tGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg                        >  2:497346/1‑100 (MQ=255)
                                                                tGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTggg                        <  2:1124474/100‑1 (MQ=255)
                                                                 gATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt                       >  1:1230592/1‑100 (MQ=255)
                                                                  aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTGTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                      <  1:937741/100‑1 (MQ=255)
                                                                   tCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTAc                     >  2:1116937/1‑100 (MQ=255)
                                                                    cACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACg                    >  1:2414304/1‑100 (MQ=255)
                                                                     aCCCCGGTCTTCAAGCCAATTGGCATGCATGACGATAACTGGCAGGCAACGGGTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                      <  1:937724/97‑1 (MQ=255)
                                                                      ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                  >  2:2443016/1‑100 (MQ=255)
                                                                       cccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTc                 >  2:1027573/1‑100 (MQ=255)
                                                                        ccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                <  2:624313/100‑1 (MQ=255)
                                                                         cGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa               <  2:1536502/100‑1 (MQ=255)
                                                                             cTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc           >  2:2352449/1‑100 (MQ=255)
                                                                              ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc          >  2:2187820/1‑100 (MQ=255)
                                                                               tCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct         >  1:1366035/1‑100 (MQ=255)
                                                                               tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct         >  2:1316960/1‑100 (MQ=255)
                                                                               tCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct         <  1:1027570/100‑1 (MQ=255)
                                                                               atctgCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct         <  1:2204528/96‑1 (MQ=255)
                                                                                cAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc        >  2:1896546/1‑100 (MQ=255)
                                                                                   gCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGGAAGAAGTGTTAGTGGGTACGCTCAACACCCTCTac     <  2:1580731/100‑1 (MQ=255)
                                                                                   gCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctagat     >  2:2204543/1‑96 (MQ=255)
                                                                                   gCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTac     <  2:327193/100‑1 (MQ=255)
                                                                                      gaTTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGTCTGTTTACAGGCGCAAGGGCGAAAGAACTGGTGGAGGGCAAGCTCAACAACCTCTACAcc  >  1:1970478/2‑100 (MQ=255)
                                                                                      aaTTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac   >  2:1792240/1‑99 (MQ=255)
                                                                                      aaTTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc  <  1:170815/100‑1 (MQ=255)
                                                                                             |                                                                                            
TTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACC  >  NC_000913/3542000‑3542185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A