Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I1 R1 118 120.6 6073099 93.5% 5678347 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,542,093 G→A V563M (GTG→ATG)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,0930GA97.9% 324.1 / ‑3.2 97V563M (GTG→ATG) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (46/49);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCA  >  NC_000913/3541994‑3542188
                                                                                                   |                                                                                               
aaCAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                 >  1:1209954/1‑100 (MQ=255)
  cAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATg                                                                                               <  1:2882166/100‑1 (MQ=255)
   aGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGc                                                                                              <  1:240580/100‑1 (MQ=255)
   aGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGc                                                                                              <  2:909593/100‑1 (MQ=255)
    gCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCa                                                                                             >  1:2632518/1‑100 (MQ=255)
      ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGcatgcatg                                                                                           <  2:2823908/100‑1 (MQ=255)
       tctcGCTGAGCGGGAAAATCGTCGATAACATCGACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCatgaatga                                                                                          >  1:2878552/1‑100 (MQ=255)
        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGaa                                                                                         <  2:3003759/100‑1 (MQ=255)
          cGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGa                                                                                       >  1:2960728/1‑100 (MQ=255)
             tGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCATCAGCCGGGTGATCACCCCGGTCTTCACGCCAATTGGCATGCATGAAGaca                                                                                     >  2:738337/1‑97 (MQ=255)
               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                                  >  1:1740755/1‑100 (MQ=255)
               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACt                                                                                  <  1:424608/100‑1 (MQ=255)
                gCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg                                                                                 <  2:1702196/100‑1 (MQ=255)
                 cGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTg                                                                                 >  2:1513273/1‑99 (MQ=255)
                  gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggc                                                                               <  1:2892887/100‑1 (MQ=255)
                  gggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggc                                                                               >  1:1857711/1‑100 (MQ=255)
                   ggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggca                                                                              <  1:2469746/100‑1 (MQ=255)
                    gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcan                                                                             <  1:924048/100‑2 (MQ=255)
                     aaaaTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTggcagg                                                                            <  2:1621251/100‑1 (MQ=255)
                      aagtcgtcgATAACAGCAACGACTCGGCGCTGGGGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTgggaggc                                                                           <  2:851498/97‑1 (MQ=255)
                         tcgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAAc                                                                        >  1:1982946/1‑100 (MQ=255)
                          cgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACg                                                                       >  2:2215584/1‑100 (MQ=255)
                           gtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACgg                                                                      <  1:1704798/100‑1 (MQ=255)
                              gATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTg                                                                   >  2:806072/1‑100 (MQ=255)
                               aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                  <  2:1383496/100‑1 (MQ=255)
                                tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                 <  1:698502/100‑1 (MQ=255)
                                tAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                 <  2:168488/100‑1 (MQ=255)
                                tAACATCAACGACGCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                 <  1:298291/100‑1 (MQ=255)
                                 aaCATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGcc                                                                <  1:2334100/100‑1 (MQ=255)
                                     tCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGtt                                                            <  1:2710185/100‑1 (MQ=255)
                                      cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                                                           <  1:88121/100‑1 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                          >  2:807577/1‑100 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                          >  1:2680553/1‑100 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                                                          >  2:1986049/1‑100 (MQ=255)
                                       aaCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTGTa                                                          >  1:2840705/1‑100 (MQ=255)
                                        aCGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTAc                                                         >  2:210874/1‑100 (MQ=255)
                                         cGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACa                                                        <  1:2937584/100‑1 (MQ=255)
                                           aCTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg                                                      >  2:1341735/1‑100 (MQ=255)
                                            cTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAgg                                                      <  1:362063/99‑1 (MQ=255)
                                             tCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                                                    <  2:1740740/100‑1 (MQ=255)
                                              cGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                                                   <  2:195594/100‑1 (MQ=255)
                                               ggCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                  <  2:1613544/100‑1 (MQ=255)
                                                    tgtcgtccttcAGCCTGTTGATCACCCCGGTCTTCAAGCCAATTGTCATTCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc                                             <  2:2878526/97‑1 (MQ=255)
                                                     ggCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg                                            >  2:1512405/1‑100 (MQ=255)
                                                       cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                          >  1:2860996/1‑100 (MQ=255)
                                                       cgtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                          >  1:2860222/1‑100 (MQ=255)
                                                        gtccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa                                         <  1:820541/100‑1 (MQ=255)
                                                         tccgtcAGTTGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaag                                        >  1:898895/1‑100 (MQ=255)
                                                          ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga                                       <  1:773399/100‑1 (MQ=255)
                                                          ccgtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga                                       >  1:2581237/1‑100 (MQ=255)
                                                            gtcAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                     <  2:379849/100‑1 (MQ=255)
                                                              cAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                   >  1:1401120/1‑100 (MQ=255)
                                                              cAGCCGGATGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTg                                   >  1:1594207/1‑100 (MQ=255)
                                                                  ggggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                               >  1:824922/2‑100 (MQ=255)
                                                                  ggggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                               >  1:824961/2‑100 (MQ=255)
                                                                  cGGGTGATCACCCCGGTCTTCAAGCCGATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                               >  1:1849257/1‑100 (MQ=255)
                                                                  cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                               >  1:2425691/1‑100 (MQ=255)
                                                                  cGGGTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                               >  1:2426317/1‑100 (MQ=255)
                                                                    ggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                             <  2:1286287/100‑1 (MQ=255)
                                                                    ggTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTg                             <  2:1437578/100‑1 (MQ=255)
                                                                     gTGATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTgg                            >  2:1209643/1‑100 (MQ=255)
                                                                       gATCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt                          >  1:358492/1‑100 (MQ=255)
                                                                        aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                         >  1:2853727/1‑100 (MQ=255)
                                                                        aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                         <  2:3016216/100‑1 (MQ=255)
                                                                        aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                         <  1:1986067/100‑1 (MQ=255)
                                                                        aTCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTa                         >  1:1701597/1‑100 (MQ=255)
                                                                         tCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTAc                        >  2:1960160/1‑100 (MQ=255)
                                                                         tCACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTAc                        <  1:1539985/100‑1 (MQ=255)
                                                                          cACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACg                       >  1:696791/1‑100 (MQ=255)
                                                                          cACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACg                       >  1:1786277/1‑100 (MQ=255)
                                                                          cACCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACg                       >  2:1311036/1‑100 (MQ=255)
                                                                           aCCCCGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGc                      >  1:254819/1‑100 (MQ=255)
                                                                            ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGc                      >  2:478111/1‑99 (MQ=255)
                                                                            ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                     <  1:2389090/100‑1 (MQ=255)
                                                                            ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                     >  2:46566/1‑100 (MQ=255)
                                                                            ccccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCt                     >  2:386999/1‑100 (MQ=255)
                                                                             cccGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTc                    <  2:198581/100‑1 (MQ=255)
                                                                               cGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa                  <  2:1583018/100‑1 (MQ=255)
                                                                               cGGTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCaa                  <  1:806079/100‑1 (MQ=255)
                                                                                ggTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                 >  2:593499/1‑100 (MQ=255)
                                                                                ggTCTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                 <  2:152914/100‑1 (MQ=255)
                                                                                 gTCCTCAAGCCAATTGGCATGCATGAAGAGAGCTGGCAGGCAACGGTTGGCCTGGTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTGAaca                <  2:669849/100‑1 (MQ=255)
                                                                                   cTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc              >  1:265087/1‑100 (MQ=255)
                                                                                   cTTCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc              >  2:1462900/1‑100 (MQ=255)
                                                                                    ttCAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAccc             <  1:1347134/100‑1 (MQ=255)
                                                                                      cAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc           >  2:1463/1‑100 (MQ=255)
                                                                                      cAAGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctc           <  1:1780499/100‑1 (MQ=255)
                                                                                       aaGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct          <  1:2645367/100‑1 (MQ=255)
                                                                                       aaGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct          <  2:696784/100‑1 (MQ=255)
                                                                                       aaGCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct          <  1:1709966/100‑1 (MQ=255)
                                                                                         gCCAATTGGCATGCATGAAGATAACTGTCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTac        <  2:1498061/100‑1 (MQ=255)
                                                                                         gCCAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTac        <  2:2301276/100‑1 (MQ=255)
                                                                                          ccAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCGCTaca       <  2:2539985/100‑1 (MQ=255)
                                                                                          ccAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca       >  1:1971005/1‑100 (MQ=255)
                                                                                           cAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac      <  1:2473901/100‑1 (MQ=255)
                                                                                           cAATTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac      <  2:1849241/100‑1 (MQ=255)
                                                                                            aaTTGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACAcc     >  1:1581130/1‑100 (MQ=255)
                                                                                              ttGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTacac      <  2:950957/97‑1 (MQ=255)
                                                                                              gtGGCATGCATGAGGGGAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCAGGGCGAAAGAAGTGGTAGTGGGTACGCTCAAGTCCCGCTACAACGc   <  2:2239417/99‑1 (MQ=255)
                                                                                               tGGCATGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCa  >  1:1246043/1‑100 (MQ=255)
                                                                                                   |                                                                                               
AACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCA  >  NC_000913/3541994‑3542188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A