Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F200 I1 R1
|
100 |
75.0 |
3643689 |
96.7% |
3523447 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,512,561 |
Δ1 bp |
intergenic (‑217/+73) |
yhfZ ← / ← trpS |
putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,512,560 | 0 | T | . | 96.0%
| 208.8
/ 2.3
| 50 | intergenic (‑216/+74) | yhfZ/trpS | putative DNA‑binding transcriptional regulator/tryptophanyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (23/25); total (23/27) |
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 |
TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCA > NC_000913/3512462‑3512655
|
tAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAta > 2:1168722/1‑99 (MQ=255)
aaaaaaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCAtac < 1:96948/100‑3 (MQ=255)
aaaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTg < 1:118231/100‑1 (MQ=255)
aaCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCTTACATTTCCTTGCAATACGTGCA‑TACtttct < 2:1224071/100‑3 (MQ=37)
aCCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTa < 1:163059/100‑1 (MQ=255)
ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAg > 1:684141/1‑100 (MQ=255)
ccGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAg > 2:1204224/1‑100 (MQ=255)
ggTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGcc > 2:1429024/1‑100 (MQ=255)
tCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGAt > 1:280492/1‑100 (MQ=255)
cccGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATa < 1:1239382/99‑1 (MQ=255)
cGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAgg < 1:1050150/100‑1 (MQ=255)
tttCAGAGAGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGTCCTTGCAATCTGTGCA‑TACTTTGTAGGCCGGATAAGGCGt < 1:1786034/100‑1 (MQ=25)
ttCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGtt > 1:1802658/1‑100 (MQ=255)
cAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCa > 2:791113/1‑100 (MQ=255)
agagTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAc > 1:1603495/1‑100 (MQ=255)
gagTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAcg < 2:1598764/100‑1 (MQ=255)
gatgatAAAAGCAAAATTGCCTGATGCGCTACGCTTATAAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCAcgccgc > 1:28925/1‑100 (MQ=255)
gatAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1485207/1‑100 (MQ=255)
atAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:38911/1‑98 (MQ=255)
tAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 2:1399228/1‑100 (MQ=255)
cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGTTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa < 2:1730952/100‑1 (MQ=255)
aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc < 2:809188/100‑1 (MQ=255)
aTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa > 2:359007/1‑100 (MQ=255)
aTTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACaa < 1:286441/100‑1 (MQ=255)
tGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg < 2:1624181/100‑1 (MQ=255)
cTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAgcgc < 2:933687/100‑1 (MQ=255)
gcTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGc > 1:1047425/1‑100 (MQ=255)
cTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc < 1:1567092/100‑1 (MQ=255)
cTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc > 1:1763185/1‑100 (MQ=255)
tACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGcc < 2:1506939/100‑1 (MQ=255)
cGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAg < 2:1199609/100‑1 (MQ=255)
gCTTATCAGGCCTACATTTCCTTTCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGc < 1:1368537/99‑1 (MQ=255)
gCTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCa < 1:166735/100‑1 (MQ=255)
cTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCaa < 1:766784/100‑1 (MQ=255)
cTTATCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCaa > 1:137732/1‑100 (MQ=255)
aTCAGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGACATGAACAAAGCGCAATTTGCCAGCAATAg > 2:40303/1‑100 (MQ=255)
aGGCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGa > 1:204836/1‑100 (MQ=255)
ggCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTg < 1:123425/98‑1 (MQ=255)
ggCCTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGaa > 1:1612504/1‑100 (MQ=255)
cTACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTa > 2:1262321/1‑100 (MQ=255)
tACATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTAc > 2:1603856/1‑100 (MQ=255)
aCATTTCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAATGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACg < 1:587194/100‑1 (MQ=255)
ttCCTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCtt < 2:1196575/100‑1 (MQ=255)
tCCTTGCAATATGTGCA‑TACTTTCTGGGCTGGATAAAGCGTTCCCGCAGCATCAGCTATTAACAAAGCTCAATTTGCCAGTAATAGAGAATTACGGCt < 1:1586834/98‑1 (MQ=255)
cTTGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGc < 2:1802671/100‑1 (MQ=255)
ttGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGcc < 2:137732/100‑1 (MQ=255)
ttGCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGc < 1:547044/99‑1 (MQ=255)
gCAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCcac > 2:1531745/1‑100 (MQ=255)
cAATATGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCcaca > 1:784417/1‑100 (MQ=255)
atatGTGCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACaaa < 2:922749/100‑1 (MQ=255)
tgtgCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAAcc < 2:1577417/100‑1 (MQ=255)
gtgCA‑TACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCa > 2:1098181/1‑100 (MQ=255)
|
TAAAAAAACCGGGTTTCCCCGGTTTCAGAGTGATGATAAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATTTCCTTGCAATATGTGCATTACTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCGCAATTTGCCAGCAATAGTGAATTACGGCTTCGCCACAAAACCA > NC_000913/3512462‑3512655
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
