Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F183 I1 R1 63 136.1 6714777 94.6% 6352179 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,397,557 G→T G49V (GGT→GTT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,5570GT96.8% 521.6 / 3.9 157G49V (GGT→GTT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (79/73);  total (81/76)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 157 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652
                                                                                                  |                                                                                               
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               <  2:282536/100‑1 (MQ=255)
 gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              <  1:3036649/100‑1 (MQ=255)
 gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              <  2:1196359/100‑1 (MQ=255)
    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                           >  1:2853323/1‑100 (MQ=255)
      gtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGa                                                                                         >  1:2756177/1‑100 (MQ=255)
       tggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaa                                                                                        <  2:715078/100‑1 (MQ=255)
        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGaaa                                                                                       >  1:1581381/1‑100 (MQ=255)
         gtCGAGCCACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                      <  2:2312864/100‑1 (MQ=255)
           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                    >  1:1779287/1‑100 (MQ=255)
           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                    >  2:3085443/1‑100 (MQ=255)
             aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAt                                                                                  <  2:2190751/100‑1 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCCGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                               >  1:239393/1‑100 (MQ=255)
                      cGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCg                                                                         <  1:886656/100‑1 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       >  2:3291789/1‑100 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       <  2:2397602/100‑1 (MQ=255)
                         cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      <  2:2875655/100‑1 (MQ=255)
                         cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      <  1:2873221/100‑1 (MQ=255)
                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     >  1:421332/1‑100 (MQ=255)
                           gTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATa                                                                    >  1:888108/1‑100 (MQ=255)
                           gTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGAAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATGCGTGATa                                                                    >  1:2300771/1‑100 (MQ=255)
                             aGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                                  <  2:2157659/100‑1 (MQ=255)
                             aGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                                  >  1:2340742/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATAGCGA‑ACGCGTGGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  2:2801024/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:253583/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               <  1:2536705/100‑1 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1724415/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:2905219/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:1364402/1‑100 (MQ=255)
                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTg                                                             >  1:1856526/1‑100 (MQ=255)
                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            <  1:510430/100‑1 (MQ=255)
                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            >  2:21107/1‑100 (MQ=255)
                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAAGCGTATCGATATTGATATCGA‑ACGCGTTGGGGAGAAACTTATACACATTCGTGATAAAGGCt                                                              >  2:2710287/1‑98 (MQ=255)
                                    gAACGAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑AAGCGTTGGGGCGAAACTTATCCGCATTCGCGATAACGGCTgtg                                                           <  1:2268325/100‑3 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  1:1259288/100‑1 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  1:1124255/100‑1 (MQ=255)
                                       cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        >  2:2541346/1‑100 (MQ=255)
                                        tAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAt                                                       <  1:811381/100‑1 (MQ=255)
                                         aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      <  1:1395795/100‑1 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  1:83116/100‑1 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  2:2455218/1‑100 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  1:3046980/1‑100 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  1:1663086/100‑1 (MQ=255)
                                           tGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAATCTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                    <  1:611564/100‑1 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  2:2223435/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  1:1336610/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                    <  2:2242309/99‑1 (MQ=255)
                                             gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGAAGTATAaaaa                                                  <  2:2956288/100‑1 (MQ=255)
                                               aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  1:99981/100‑1 (MQ=255)
                                                aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  2:1122898/100‑1 (MQ=255)
                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              >  2:1362213/1‑100 (MQ=255)
                                                 aCAGCCTCGATGCAGCTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              <  1:3297860/100‑1 (MQ=255)
                                                  cAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAt                                             >  2:2338363/1‑100 (MQ=255)
                                                    gCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGa                                           >  1:2234249/1‑100 (MQ=255)
                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgc                                         <  2:2319964/100‑1 (MQ=255)
                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgc                                         >  1:1637152/1‑100 (MQ=255)
                                                        gatctcAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctg                                       <  1:450934/95‑1 (MQ=255)
                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                      >  1:2596748/1‑100 (MQ=255)
                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                      <  1:2807986/100‑1 (MQ=255)
                                                          aTGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggc                                     >  1:349121/1‑100 (MQ=255)
                                                          aTGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggc                                     <  1:3291565/100‑1 (MQ=255)
                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgagatc                                  >  2:450933/1‑95 (MQ=255)
                                                              cgccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctg                                 >  2:764705/8‑100 (MQ=25)
                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  2:1993873/1‑100 (MQ=255)
                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGGATCAAAAAAGATGAgctggcgctgg                                >  2:52754/1‑100 (MQ=255)
                                                                gTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCTGTATCAAAAAAGATGAgctggcgctg                                 <  2:1598502/98‑1 (MQ=255)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  1:2004971/6‑100 (MQ=25)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  1:1060397/6‑100 (MQ=37)
                                                                  gCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                             <  1:2711855/100‑1 (MQ=255)
                                                                   cGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                            <  1:6136/100‑1 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           >  1:1688293/1‑100 (MQ=255)
                                                                    gACGCATATCGATATTGATATCGA‑ACGCGTTAGGGCGAAACTTATCCGCATTCGTGATAACGGAAAAAAAAAAAAAAAAGATGAgctggcgctggcg                              <  2:888106/97‑1 (MQ=255)
                                                                    aaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           >  1:217751/2‑100 (MQ=37)
                                                                    aaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           >  2:3006399/2‑100 (MQ=37)
                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:1496676/1‑100 (MQ=255)
                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  1:2709796/100‑1 (MQ=255)
                                                                      cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  1:879821/1‑100 (MQ=255)
                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGggctggct                        >  2:2246607/1‑99 (MQ=255)
                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       >  2:1584116/1‑100 (MQ=255)
                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       >  1:3056126/1‑100 (MQ=255)
                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   <  2:3138510/100‑1 (MQ=255)
                                                                              gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                  <  2:2170500/99‑1 (MQ=255)
                                                                              gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 <  1:1251064/100‑1 (MQ=255)
                                                                                tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               <  1:2739227/100‑1 (MQ=255)
                                                                                 atTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcca              <  1:324809/100‑1 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  1:3347613/1‑100 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             <  2:3276601/100‑1 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  2:375073/1‑100 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCATGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  2:2862828/1‑100 (MQ=255)
                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  1:2754630/100‑1 (MQ=255)
                                                                                    gATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAGCGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCGCGTCATGccacc            <  2:2736541/99‑1 (MQ=255)
                                                                                    gATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacca           >  1:1824783/1‑100 (MQ=255)
                                                                                       atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        >  2:848983/1‑100 (MQ=255)
                                                                                       atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        >  1:2457451/1‑100 (MQ=255)
                                                                                       atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        >  2:1516191/1‑100 (MQ=255)
                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  2:1049297/1‑100 (MQ=255)
                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  1:2794395/1‑100 (MQ=255)
                                                                                          gA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     >  2:661407/1‑100 (MQ=255)
                                                                                          gA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     <  2:1856519/100‑1 (MQ=255)
                                                                                           a‑aCGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCGGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAAt    <  1:1316868/100‑1 (MQ=255)
                                                                                              cgcgTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCg  <  1:3209767/100‑1 (MQ=255)
                                                                                                  |                                                                                               
GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A