Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F258 I0 R1 432 103.0 5191697 92.7% 4812703 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,397,557 G→T 100% G49V (GGT→GTT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,5570GT99.0% 337.3 / ‑4.1 106G49V (GGT→GTT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (52/52);  total (54/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 106 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652
                                                                                                  |                                                                                               
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               <  1:809124/100‑1 (MQ=255)
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               <  2:2298096/100‑1 (MQ=255)
   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            <  1:2263387/100‑1 (MQ=255)
   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            <  1:796555/100‑1 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            <  1:1535565/98‑1 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            <  1:130497/98‑1 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  2:963683/1‑100 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  2:36302/1‑100 (MQ=255)
      gtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGa                                                                                         >  2:133717/1‑100 (MQ=255)
         gtCGGGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGGGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                      <  1:710459/100‑1 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     >  1:1527424/1‑100 (MQ=255)
           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                    >  1:447096/1‑100 (MQ=255)
           cGAGCGACCTGCGCCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                    >  2:1739875/1‑100 (MQ=255)
            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTa                                                                                   <  1:802959/100‑1 (MQ=255)
             aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAt                                                                                  >  1:373123/1‑100 (MQ=255)
             aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAt                                                                                  <  1:1059783/100‑1 (MQ=255)
                gACCTTCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                               <  1:782583/100‑1 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                               >  2:452462/1‑100 (MQ=255)
                  ccTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGGGTTGGGGCGAAACTTATCCGCa                                                                             >  2:487652/1‑100 (MQ=255)
                   cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAt                                                                            >  2:2193269/1‑100 (MQ=255)
                       ctctatAGCCAAAAAACTAGTGGAAAACAGCCTCGATGCAGGTGCGCCACATAGCGCCATAGATCTCGA‑ACGCGATGGGGCGAACCTTATCCGCGtt                                                                           >  1:2366394/6‑97 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       <  1:781054/100‑1 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                       <  2:2075797/100‑1 (MQ=255)
                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     >  1:1809975/1‑100 (MQ=255)
                          ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTCGGGGCGAAACTTATCCGCATTCGTGAt                                                                     >  2:2513849/1‑100 (MQ=255)
                            tAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATaa                                                                   <  2:1478515/100‑1 (MQ=255)
                             aGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGAGATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                                  >  1:1814416/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  2:1222/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  1:1995021/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 <  2:1435255/100‑1 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACgg                                                                >  1:1032759/1‑99 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1887229/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTAGCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACggn                                                               <  1:2256258/100‑2 (MQ=255)
                                  aaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTg                                                             <  2:2114434/100‑1 (MQ=255)
                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            >  2:1263089/1‑100 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCTATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  2:1334260/100‑1 (MQ=255)
                                     aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  1:1342119/100‑1 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      >  2:534840/1‑99 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     <  2:1776258/100‑1 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  1:1899601/1‑100 (MQ=255)
                                          gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGAGATTGGTATCGA‑ACGCGTGGGGGCGCAAATTATCAGCACTCGTGATAACGGATGCGGTATc                                                      >  1:727109/1‑99 (MQ=255)
                                           tGGACAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCCTTCGTGATAACGGCTGCGGTATCaa                                                    >  2:2499261/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  1:779357/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  2:1271638/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAATTATCCGCATTAGTGATAAAGGATGCGGTATCa                                                     >  2:476527/1‑98 (MQ=255)
                                             gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                                  >  2:286963/1‑100 (MQ=255)
                                                 aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              <  2:431653/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            <  2:1841154/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            <  1:1003228/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            <  2:2029156/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            >  1:257907/1‑100 (MQ=255)
                                                     ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAACAAGATGAg                                          >  2:2331421/1‑100 (MQ=255)
                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgc                                         >  2:1041753/1‑100 (MQ=255)
                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATAACGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                          >  2:2315154/1‑99 (MQ=255)
                                                       tCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgct                                        <  1:1112379/100‑1 (MQ=255)
                                                        agATGCAGGTGCGACGCGTTTGTATAGTGATATCGA‑ACGCGTTGGGGGGAAACTTAACCGCATTCGTGATAGCGGCTGCGGTATCAAAAAAGATg                                            <  1:199568/94‑1 (MQ=255)
                                                         gATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctgg                                      <  2:1307512/100‑1 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                    <  1:1220304/100‑1 (MQ=255)
                                                             cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  >  1:2517055/1‑100 (MQ=255)
                                                              aGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctg                                 <  1:1264182/100‑1 (MQ=255)
                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                >  1:1560634/1‑100 (MQ=255)
                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                <  1:1855949/100‑1 (MQ=255)
                                                               ggTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctgg                                <  2:1888122/100‑1 (MQ=255)
                                                                gTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               <  2:569208/100‑1 (MQ=255)
                                                                gTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               <  1:384880/100‑1 (MQ=255)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  2:2291356/6‑100 (MQ=25)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  1:718657/6‑100 (MQ=25)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                               >  2:1652401/6‑100 (MQ=25)
                                                                 tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                              <  1:2054742/100‑1 (MQ=255)
                                                                 tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                              <  2:177819/100‑1 (MQ=255)
                                                                   cGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                            <  1:1263085/100‑1 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctn                           <  1:1047308/100‑2 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           >  1:1035826/1‑100 (MQ=255)
                                                                        gcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                        >  1:1961647/5‑100 (MQ=37)
                                                                        cgTATCGATATTGATCTCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       <  2:2550557/100‑1 (MQ=255)
                                                                        cgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       <  2:1911630/100‑1 (MQ=255)
                                                                        cgTATCGATATTGATATCAA‑AACCGTTGGGGCGAAAATTATCCGCATTCGTGATAACAGCTCCGGTATCAAAAAAGATAAgctggcgcgggcgc                             >  1:2437491/1‑94 (MQ=255)
                                                                         gTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCg                      <  2:843438/100‑1 (MQ=255)
                                                                         gTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCg                      <  2:1350249/100‑1 (MQ=255)
                                                                         cgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       >  2:317315/4‑100 (MQ=38)
                                                                          tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCg                      >  1:25095/1‑99 (MQ=255)
                                                                          tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGt                     >  2:1518507/1‑100 (MQ=255)
                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   <  1:36301/100‑1 (MQ=255)
                                                                            tCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCa                   <  2:1546234/100‑1 (MQ=255)
                                                                             cGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGCATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                  <  2:2278054/100‑1 (MQ=255)
                                                                                tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               <  2:2377852/100‑1 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  2:891879/1‑100 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  1:71342/1‑100 (MQ=255)
                                                                                  ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  2:1564876/1‑100 (MQ=255)
                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  2:1017836/100‑1 (MQ=255)
                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  2:866766/100‑1 (MQ=255)
                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            >  1:828808/1‑100 (MQ=255)
                                                                                   tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATCCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  1:954810/100‑1 (MQ=255)
                                                                                     atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          >  1:1276184/1‑100 (MQ=255)
                                                                                      tatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          <  2:1469496/99‑1 (MQ=255)
                                                                                      tatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGt         >  1:2015033/1‑100 (MQ=255)
                                                                                      tatCGA‑ACGCGTTGGAGCGAAACTTATCCGTATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGCCTCTGGCGCTGGCTCGTCATGCCACCAg          <  1:1171801/99‑1 (MQ=255)
                                                                                        tCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaa       >  1:2233522/1‑100 (MQ=255)
                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTGGTAATGCCACCAGTaaa      >  1:1230411/1‑100 (MQ=255)
                                                                                              cgcgTTGGGGCGAAACTTATCCGCATTCGTGATAACTGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCg  <  2:1814426/100‑1 (MQ=255)
                                                                                                  |                                                                                               
GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A