Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F244 I0 R1 270 114.4 5840133 93.3% 5448844 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,397,557 G→T 100% G49V (GGT→GTT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,397,5570GT98.4% 403.5 / ‑2.3 125G49V (GGT→GTT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (72/51);  total (72/53)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 125 total aligned reads displayed.

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAA  >  NC_000913/4397460‑4397649
                                                                                                  |                                                                                            
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                            >  1:445375/1‑100 (MQ=255)
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                            <  2:1407447/100‑1 (MQ=255)
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                            >  1:1478481/1‑100 (MQ=255)
ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGAAGCAGGTGCGACGCGCATCGATATTGACATCGA‑ACGCGTTg                                                                                            >  1:2433669/1‑100 (MQ=255)
 gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                           <  2:1300778/100‑1 (MQ=255)
  tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTggg                                                                                          <  2:1909012/100‑1 (MQ=255)
   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                         >  2:2613684/1‑100 (MQ=255)
   gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTCCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                         >  1:91521/1‑100 (MQ=255)
    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                        <  2:2234143/100‑1 (MQ=255)
    aggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGc                                                                                        <  2:314492/100‑1 (MQ=255)
     ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                       >  2:2291673/1‑100 (MQ=255)
       tggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaa                                                                                     <  2:1603350/100‑1 (MQ=255)
        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                    >  2:2324101/1‑100 (MQ=255)
        ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                    >  2:2248022/1‑100 (MQ=255)
         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                   <  2:511071/100‑1 (MQ=255)
         gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                   <  2:1936493/100‑1 (MQ=255)
          tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCATGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                  <  1:1614829/100‑1 (MQ=255)
           cGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACtt                                                                                 >  2:775443/1‑100 (MQ=255)
            gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTa                                                                                <  2:2404640/100‑1 (MQ=255)
             aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAt                                                                               >  2:1740274/1‑100 (MQ=255)
              gCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATc                                                                              <  1:2291658/100‑1 (MQ=255)
              gCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATc                                                                              <  1:1465713/100‑1 (MQ=255)
               cGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATcc                                                                             <  2:512714/100‑1 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                            <  2:2097619/100‑1 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                            >  1:2741493/1‑100 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                            <  2:2156628/100‑1 (MQ=255)
                gACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCg                                                                            <  2:2230281/100‑1 (MQ=255)
                    tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCa                                                                          >  1:1044694/1‑98 (MQ=255)
                    tGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAt                                                                         >  1:149066/1‑99 (MQ=255)
                     gCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTc                                                                       >  2:815262/1‑100 (MQ=255)
                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                     >  2:712160/1‑100 (MQ=255)
                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                     >  1:2364574/1‑100 (MQ=255)
                       gTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGt                                                                     >  2:2460557/1‑100 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                    <  2:639973/100‑1 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                    <  2:2094143/100‑1 (MQ=255)
                        tCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTg                                                                    <  2:1575540/100‑1 (MQ=255)
                           gTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATa                                                                 >  2:447595/1‑100 (MQ=255)
                             aGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCAATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                               <  1:2767021/100‑1 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                              <  2:2573932/100‑1 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                              >  1:1605645/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                              >  1:2373905/1‑100 (MQ=255)
                              gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGAGGCGAAACTTATCCGCATTCGTTATAACg                                                              >  1:930144/1‑100 (MQ=255)
                               tCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACgg                                                             >  2:2709254/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGACACTTATCCGCATTCGTGATAACgg                                                             >  1:673634/1‑99 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                            >  1:2141263/1‑100 (MQ=255)
                                cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                            >  2:669188/1‑100 (MQ=255)
                                 aaaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCt                                                           >  2:2472751/1‑100 (MQ=255)
                                 aaaGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCt                                                           <  2:1838581/100‑1 (MQ=255)
                                   aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                         <  1:2307631/100‑1 (MQ=255)
                                      aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                      <  2:2071390/100‑1 (MQ=255)
                                       cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                      >  1:1147483/1‑99 (MQ=255)
                                          gTGGAAAACAGAATCGATGCAGATGCGACGAGTATCGATATTGATATCGG‑ACGCGTTGGCGCGATACTTATCCGCATTCGTGATAAAAGCTACGGTaaaa                                                  >  1:1575725/1‑97 (MQ=255)
                                           tGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                 >  2:2424665/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                >  1:670651/1‑100 (MQ=255)
                                            ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                >  1:662878/1‑100 (MQ=255)
                                             gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                               <  2:1546053/100‑1 (MQ=255)
                                             gAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                               <  2:1180834/100‑1 (MQ=255)
                                              aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                              >  2:1240052/1‑100 (MQ=255)
                                               aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                             <  2:156185/100‑1 (MQ=255)
                                                  cAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAt                                          <  2:2373920/100‑1 (MQ=255)
                                                  cAGCCTCGATGCAGGAGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAt                                          >  1:2913587/1‑100 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                         <  2:2073017/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                         <  2:1413860/100‑1 (MQ=255)
                                                   aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                         <  1:990537/100‑1 (MQ=255)
                                                     ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                       >  2:2027155/1‑100 (MQ=255)
                                                      cTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgc                                      >  2:1861919/1‑100 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                 >  2:747088/1‑100 (MQ=255)
                                                           tGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcg                                 >  1:667029/1‑100 (MQ=255)
                                                                gTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                            >  2:1155856/1‑100 (MQ=255)
                                                                ccccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                            >  2:2114620/6‑100 (MQ=25)
                                                                 tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                           <  2:2555512/100‑1 (MQ=255)
                                                                 tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                           <  2:2785699/100‑1 (MQ=255)
                                                                  gCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                          >  2:630371/1‑100 (MQ=255)
                                                                  gCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggc                            >  2:660220/1‑98 (MQ=255)
                                                                  ccaaCGCGT‑TCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                          >  2:2022667/4‑100 (MQ=37)
                                                                  ccaaCGCGT‑TCGATATCAATATCGATACGCGTAGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgc                          >  2:2765059/4‑100 (MQ=25)
                                                                   cGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTGTCAAAAAAGATGAgctggcgctggcgct                         >  1:1342861/1‑100 (MQ=255)
                                                                   cGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                         <  2:2083229/100‑1 (MQ=255)
                                                                    gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCTTGATAACGGCTGCGGTATCAAAAAAGATGAgcttgcgctggcgctg                        <  2:1102128/100‑1 (MQ=255)
                                                                     acacgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                        <  1:2099417/96‑1 (MQ=255)
                                                                     aCGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctgg                       <  2:2252197/100‑1 (MQ=255)
                                                                     aCGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATC‑AAAAAGATGAgctggcgctggcgctggc                      <  2:1447166/100‑1 (MQ=255)
                                                                       gcgTATCGATATTGATAGCGA‑ACGCGTTGGGGCGAAACTAAGCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                     <  1:2445820/100‑1 (MQ=255)
                                                                       cgcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                      >  2:295570/6‑100 (MQ=37)
                                                                       cgcgttCGATATCAATATCGATACGCGTCGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                      >  2:443172/6‑100 (MQ=25)
                                                                        gcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                     >  1:1682904/5‑100 (MQ=37)
                                                                        gcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCt                     >  1:296596/5‑100 (MQ=38)
                                                                        gcgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAACTGGCGCTGGCGCTGGCt                     >  1:2201638/5‑100 (MQ=37)
                                                                         cgttCGATATCAATATCGATACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                    >  1:1490781/4‑100 (MQ=38)
                                                                           aTCGATATTGATATCGA‑TCGCGTTGGGTCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgccagcgccagcgccagctcatc                 <  1:211871/100‑10 (MQ=255)
                                                                           aTCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                 >  2:2064826/1‑100 (MQ=255)
                                                                           aTCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTc                 <  2:2329023/100‑1 (MQ=255)
                                                                             cGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt               >  1:2675565/1‑100 (MQ=255)
                                                                              gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAAATGAGCTGGCGCTGGCGCTGGCTCGTGAt               >  1:2635127/1‑99 (MQ=255)
                                                                              gATATTGATATCGA‑ACGCGTTAGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAACAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt               >  1:2917209/1‑99 (MQ=255)
                                                                                tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc            >  1:10036/1‑100 (MQ=255)
                                                                                    gATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacca        >  1:2525453/1‑100 (MQ=255)
                                                                                        tCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaa    >  2:1794310/1‑100 (MQ=255)
                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa   <  1:2514419/100‑1 (MQ=255)
                                                                                         cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa   >  1:935968/1‑100 (MQ=255)
                                                                                                  |                                                                                            
GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAA  >  NC_000913/4397460‑4397649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A