Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I1 R1 174 80.0 3892211 96.7% 3763768 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,707,029 A→G V37V (GTA→GTG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,707,0290AG95.7% 231.0 / ‑1.6 70V37V (GTA→GTG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (34/33);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAG  >  NC_000913/1706933‑1707125
                                                                                                |                                                                                                
ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCACCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGccc                                                                                               >  2:978250/1‑100 (MQ=255)
  ctGCATTCAGAAAAAATAAAATCTGGGATTTCACCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCt                                                                                             >  1:1032852/1‑100 (MQ=255)
   tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTg                                                                                            <  2:589452/100‑1 (MQ=255)
   tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTg                                                                                            <  1:1696118/100‑1 (MQ=255)
    gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCACTGCGCCAGGTGCCCCTgg                                                                                           >  2:781607/1‑100 (MQ=255)
         cAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAg                                                                                      <  2:642286/100‑1 (MQ=255)
         cAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAg                                                                                      >  1:1835026/1‑100 (MQ=255)
           gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCg                                                                                    >  1:1575895/1‑100 (MQ=255)
            aaaaaaTAAAATCTGGGATTTCAACGGCGGCCTCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCGGGCGCAGCg                                                                                    >  1:185015/1‑99 (MQ=255)
             aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGtt                                                                                  >  1:232934/1‑100 (MQ=255)
              aaaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGttt                                                                                 <  2:1214978/100‑1 (MQ=255)
               aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGtttt                                                                                <  1:1688273/100‑1 (MQ=255)
                 ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGt                                                                              <  2:1809299/100‑1 (MQ=255)
                   aaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTa                                                                            <  2:1732906/100‑1 (MQ=255)
                    aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                           >  2:1555168/1‑100 (MQ=255)
                       tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTcc                                                                        <  1:1677182/100‑1 (MQ=255)
                       tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCACCTGGCGCAGCGTTTTGTTATTc                                                                         >  1:249617/1‑99 (MQ=255)
                        cTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCa                                                                       >  2:1245996/1‑100 (MQ=255)
                         tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCGAACGGTACACCCCTACGCCAGGTGCCCCTGGCGCAGGGTTTTGTTGTTCCAc                                                                      >  1:48864/1‑100 (MQ=255)
                         tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCAc                                                                      <  2:212987/100‑1 (MQ=255)
                          gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACt                                                                     <  2:1142437/100‑1 (MQ=255)
                           ggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                    <  2:914855/100‑1 (MQ=255)
                           ggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTg                                                                    <  2:217423/100‑1 (MQ=255)
                             aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGaa                                                                  >  2:502521/1‑100 (MQ=255)
                                tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa                                                               >  2:91498/1‑100 (MQ=255)
                                 cAACGGCGGCATCAATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                              >  2:1306769/1‑100 (MQ=255)
                                  aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc                                                             <  1:615728/100‑1 (MQ=255)
                                   aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                            <  2:1528661/100‑1 (MQ=255)
                                    cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCat                                                           <  2:636137/100‑1 (MQ=255)
                                    cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCat                                                           <  2:1864550/100‑1 (MQ=255)
                                        ggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAttn                                                       <  1:753193/100‑2 (MQ=255)
                                              catccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTg                                                 >  2:344198/1‑100 (MQ=255)
                                                   aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt                                            <  2:1270667/100‑1 (MQ=255)
                                                      ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg                                         >  2:1631896/1‑100 (MQ=255)
                                                       gagaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGCGAg                                         >  1:120030/1‑99 (MQ=255)
                                                          aTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgt                                     >  1:1436465/1‑100 (MQ=255)
                                                           tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg                                    >  2:537170/1‑100 (MQ=255)
                                                           tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg                                    <  1:112526/100‑1 (MQ=255)
                                                           tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg                                    >  1:1806235/1‑100 (MQ=255)
                                                           tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTCATTCCACTGGAACAGCATACTGGCGCTGAAGGTGAGTtgtg                                    >  1:1289200/1‑100 (MQ=255)
                                                             aaaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg                                  <  2:1883428/100‑1 (MQ=255)
                                                                aCCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTTAGTTGTGCGTTa                               <  2:1280760/100‑1 (MQ=255)
                                                                 cccAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                              <  1:411625/100‑1 (MQ=255)
                                                                 cccAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                              <  2:21620/100‑1 (MQ=255)
                                                                   cAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTTGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                            <  1:338074/100‑1 (MQ=255)
                                                                     gTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc                          >  2:1333489/1‑100 (MQ=255)
                                                                      tCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCg                         <  1:502519/100‑1 (MQ=255)
                                                                       ccAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                        >  1:29959/1‑100 (MQ=255)
                                                                       ccAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCgg                        >  1:1566267/1‑100 (MQ=255)
                                                                        cAACGGCACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGc                       <  2:249619/100‑1 (MQ=255)
                                                                          aCGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTTAGTTGTGCGTTAGGGTc                          >  1:769625/1‑95 (MQ=255)
                                                                          aCGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                     <  2:790815/100‑1 (MQ=255)
                                                                          aCGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                     >  1:768785/1‑100 (MQ=255)
                                                                            ggTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATa                   <  1:684961/100‑1 (MQ=255)
                                                                               acacCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                <  2:220218/100‑1 (MQ=255)
                                                                               acacCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg                <  2:1032853/100‑1 (MQ=255)
                                                                                cacCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGt               >  2:1469350/1‑100 (MQ=255)
                                                                                  ccccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt             >  2:34321/1‑100 (MQ=255)
                                                                                  ccccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt             <  1:656669/100‑1 (MQ=255)
                                                                                  ccccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt             >  2:822952/1‑100 (MQ=255)
                                                                                  ccccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt             >  2:959510/1‑100 (MQ=255)
                                                                                    gcTGCGCCAGGGGCACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGCAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg           >  1:1603699/2‑100 (MQ=255)
                                                                                    ccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg           <  1:1731701/100‑1 (MQ=255)
                                                                                    ccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGAGAtcggaagagc           >  1:1561208/1‑91 (MQ=255)
                                                                                     cTGCGCCAGGGGCACCTGGCGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc          >  1:1899976/1‑100 (MQ=255)
                                                                                       gcgcCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc        >  1:258164/1‑100 (MQ=255)
                                                                                        cgcCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGcgcg       >  2:1513135/1‑100 (MQ=255)
                                                                                         gcCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCgg      <  2:1408489/100‑1 (MQ=255)
                                                                                          ccAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc     <  1:574756/100‑1 (MQ=255)
                                                                                             ggTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAg  >  2:1282608/1‑100 (MQ=255)
                                                                                                |                                                                                                
CTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAG  >  NC_000913/1706933‑1707125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A