Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I0 R1
|
175 |
91.2 |
4812830 |
94.5% |
4548124 |
99.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,707,029 |
A→G |
19.9% |
V37V (GTA→GTG) |
rsxC → |
SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,707,029 | 0 | A | G | 19.9%
| 109.5
/ 19.1
| 55 | V37V (GTA→GTG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base A (23/21); new base G (7/4); total (30/25) |
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCC > NC_000913/1706933‑1707127
|
ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc > 2:629318/1‑100 (MQ=255)
ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc < 1:964276/100‑1 (MQ=255)
ctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt < 1:1694010/100‑1 (MQ=255)
cATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgc < 2:747045/100‑1 (MQ=255)
aTTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgcg > 1:597777/1‑100 (MQ=255)
tCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCa < 2:654714/100‑1 (MQ=255)
cAGAAAAAATAAAATCTGCGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGgcgccg > 2:1206088/1‑98 (MQ=255)
aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc > 1:340199/1‑100 (MQ=255)
aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc > 1:639752/1‑100 (MQ=255)
gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAAAGGTAAACCCCTCCGAAAGGTGCCCCTGCCGCAGCg > 2:1236650/1‑100 (MQ=255)
aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg < 1:851954/100‑1 (MQ=255)
aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTg < 2:1031696/100‑1 (MQ=255)
ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt < 1:22001/100‑1 (MQ=255)
taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACACCTGGAGCAGCGTTTTGt > 2:841475/1‑99 (MQ=255)
caaaatCTGGGATCTCAACGGCGGCATACATCCACCGGAGATGAAAACCCAGTCCAACAGCACACCCATGCGCCAGGTGCCCCTGGCGTAGc > 2:396168/2‑92 (MQ=255)
tCTGGGTTTTCAACTGTGGCATCCATCCACCGTAGATGAAAACCCAGTCCAACTGTACACTCCTGCGCCAGGTACCCTTTGCGCAGCGTTTTGTTATTcc < 2:360631/100‑1 (MQ=255)
tCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTcc < 1:1392686/100‑1 (MQ=255)
tGGGATTTCAACGGCGGCATCCATCAACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGGCAGATACCCCTGGCGCAGCGTTTTGTTATTCCAc > 2:2010634/1‑100 (MQ=255)
gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACt < 1:1012663/100‑1 (MQ=255)
ggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTg < 1:1184233/100‑1 (MQ=255)
tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaaa > 1:1156303/1‑100 (MQ=255)
tCAACGGCGGCATTCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa < 1:731735/100‑1 (MQ=255)
tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa > 1:1955580/1‑100 (MQ=255)
cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACa > 1:269/1‑99 (MQ=255)
aaCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc < 1:1585400/100‑1 (MQ=255)
cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCat < 2:1586797/100‑1 (MQ=255)
gtatccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg < 1:1920261/98‑1 (MQ=255)
gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg < 2:392319/100‑1 (MQ=255)
aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAgg < 2:880947/99‑1 (MQ=255)
ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg > 1:486865/1‑100 (MQ=255)
ccGGAGATCAAAACCCAGTCCAACGGTACACCCCTGCGCAAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt < 2:550414/99‑1 (MQ=255)
ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAg < 1:2232612/100‑1 (MQ=255)
gagaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGt < 1:1937066/100‑1 (MQ=255)
tGAAACCCCAGTCCAACGGTAAACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAAAAGCATAGCGGCGCTGAagttgagttg > 2:458305/1‑98 (MQ=255)
tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg < 2:2364143/100‑1 (MQ=255)
tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAACCATCATATTGGCGCTGAAGGTGAGTtgtg > 1:384260/1‑100 (MQ=255)
aaaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg > 2:591741/1‑100 (MQ=255)
aaaCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGt > 1:953750/1‑100 (MQ=255)
aaCCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCg > 2:641333/1‑98 (MQ=255)
aCCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa > 2:747722/1‑100 (MQ=255)
aGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTTTTTTTTTTTTTAAATAGAATATTGGCGCTGAAGGTGAGTTGTGGGTTAgtgt > 2:1377391/1‑100 (MQ=255)
gTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc < 1:1003793/100‑1 (MQ=255)
cAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTCTTATTCCACTGCACCAGCATACTGGCGCCGAAGCTGCCCTGTGCGTTACCGTc > 2:644444/1‑97 (MQ=255)
aCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa > 1:2394570/1‑100 (MQ=255)
acacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAg > 2:552233/1‑100 (MQ=255)
ccccTGCGCCAGGTGCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt < 1:1674493/100‑1 (MQ=255)
ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt > 2:1692250/1‑100 (MQ=255)
cgcTGCGCCAGGGGTACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt > 2:1863565/3‑100 (MQ=38)
cgcTGCGCCAGGGGCACCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAtt > 1:21256/3‑100 (MQ=255)
cccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGACGGCGATAAAGTAtt > 1:646022/1‑100 (MQ=255)
ccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg > 2:1746844/1‑100 (MQ=255)
gcgcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc > 2:1380023/1‑100 (MQ=255)
cgcCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGcgcg > 1:2153406/1‑100 (MQ=255)
tACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc < 2:340196/100‑1 (MQ=255)
tACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGcc < 1:552236/100‑1 (MQ=255)
|
CTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCC > NC_000913/1706933‑1707127
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
