Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F142 I0 R1
|
175 |
91.2 |
4812830 |
94.5% |
4548124 |
99.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,420,727 |
G→A |
24.8% |
G36D (GGC→GAC) |
yfcG → |
GSH‑dependent disulfide bond oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,420,727 | 0 | G | A | 24.8%
| 143.9
/ 40.4
| 78 | G36D (GGC→GAC) | yfcG | GSH‑dependent disulfide bond oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (31/27); new base A (13/6); total (45/33) |
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAGCCT > NC_000913/2420628‑2420826
|
atcTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAgg < 1:2180550/100‑1 (MQ=255)
tctcTATTTCGGCCCGACACCCAATGGTCAAAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAggg < 2:530307/100‑2 (MQ=255)
tctcTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGc < 1:630641/100‑1 (MQ=255)
ctctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCg > 1:1007909/1‑100 (MQ=255)
tctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCgg < 2:1699513/100‑1 (MQ=255)
ctATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGt < 1:71672/100‑1 (MQ=255)
tATTTCGCCCTGACACCCAATGGTTACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTc < 1:9183/100‑1 (MQ=255)
aTTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCa > 1:1540216/1‑100 (MQ=255)
ttCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGt > 1:2202869/1‑100 (MQ=255)
tCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGtt > 1:267226/1‑100 (MQ=255)
gCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTc > 1:2134536/1‑100 (MQ=255)
tccGACATCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCg < 1:202322/98‑1 (MQ=255)
ccGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTc > 2:1696712/1‑100 (MQ=255)
ccGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTc > 1:1734536/1‑100 (MQ=255)
gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCg < 1:502625/100‑1 (MQ=255)
gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCg < 1:1933957/100‑1 (MQ=255)
gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCg < 1:868686/100‑1 (MQ=255)
gACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCg < 1:1475653/100‑1 (MQ=255)
acacCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCgg > 2:2282264/1‑100 (MQ=255)
acacCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCgg < 2:931178/100‑1 (MQ=255)
acacCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCgg < 1:2342630/100‑1 (MQ=255)
cacCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCgta > 1:1236307/1‑98 (MQ=255)
cccAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAAt > 2:1735749/1‑100 (MQ=255)
cccAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAAt > 2:405972/1‑100 (MQ=255)
ggTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTTGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACTGTCAGTTTCGTCCGGAATTTTTg < 2:2154864/99‑1 (MQ=255)
gTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGGTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTgcg < 2:1083824/100‑1 (MQ=255)
gTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTgcg < 2:1107551/100‑1 (MQ=255)
cacaAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCa > 2:559301/1‑100 (MQ=255)
cacaAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTAGGGAACGGCGGTCAGTTTCGTCCGGAATTTTTGCGCa > 2:1614171/1‑100 (MQ=255)
caAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGCAGGTCAGTATCGTCCGGAATTTTTGCGCAtt > 2:1614188/1‑100 (MQ=255)
aTTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGc < 2:1773846/100‑1 (MQ=255)
ttACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGcc < 1:2169225/100‑1 (MQ=255)
tACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGccc < 1:1244430/100‑1 (MQ=255)
aCGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCca < 1:541128/100‑1 (MQ=255)
aCGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCca < 2:1823354/100‑1 (MQ=255)
cGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaa > 1:1942450/1‑100 (MQ=255)
ccgatcttcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaaca < 1:798447/93‑1 (MQ=255)
cTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaaca > 2:153502/1‑100 (MQ=255)
cTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTAGCCcaaca > 1:145063/1‑100 (MQ=255)
tGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaac < 1:1825764/98‑1 (MQ=255)
gTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaac > 2:201621/1‑100 (MQ=255)
gTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaac < 2:1734515/100‑1 (MQ=255)
ttCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaacaa < 1:232838/100‑1 (MQ=255)
tcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCcaacaaggtcgg > 2:798438/1‑94 (MQ=255)
tcGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAAt > 1:670419/1‑100 (MQ=255)
gaagaaGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTc > 1:1600551/1‑100 (MQ=255)
agCAGAGCTGGATTATCGCTTGATTAAGATAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCa > 2:1066334/1‑100 (MQ=255)
cAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCaa > 2:2084206/1‑99 (MQ=255)
cAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAt > 1:2262774/1‑100 (MQ=255)
cAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAt > 2:37239/1‑100 (MQ=255)
cAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAt > 2:1732480/1‑100 (MQ=255)
agCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgt < 1:1849626/100‑1 (MQ=255)
gCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAAttgtt > 2:460793/1‑100 (MQ=255)
ggATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGAt < 2:1007898/100‑1 (MQ=255)
ggATTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGAt > 1:160807/1‑100 (MQ=255)
aTTATCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCa < 1:316762/100‑1 (MQ=255)
aTCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtc > 2:889689/1‑100 (MQ=255)
tCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtct < 2:267223/100‑1 (MQ=255)
tCGCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATtc > 2:2113294/1‑99 (MQ=255)
gCTTGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTcc > 2:732568/1‑100 (MQ=255)
ttGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTg < 1:186951/100‑1 (MQ=255)
tGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTg > 1:767530/1‑99 (MQ=255)
tGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGc < 1:535556/100‑1 (MQ=255)
tGATTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGc > 2:162810/1‑100 (MQ=255)
gATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGcc > 2:74360/1‑100 (MQ=255)
aTTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCg > 2:166492/1‑100 (MQ=255)
aTTAAGGTAGACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCg > 1:782476/1‑100 (MQ=255)
ggTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggc > 2:2289627/1‑100 (MQ=255)
aGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggcggc < 1:1696732/100‑1 (MQ=255)
aGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATggcggc < 1:405975/100‑1 (MQ=255)
gACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGgcggcg > 2:1228905/1‑100 (MQ=255)
gACCTGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGACGATGgcggcg > 1:127805/1‑100 (MQ=255)
cTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAc < 2:956771/100‑1 (MQ=255)
cTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAc > 1:936328/1‑100 (MQ=255)
tGGGGAAAGACGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAAcc > 1:254210/1‑100 (MQ=255)
ggggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg > 1:63856/1‑100 (MQ=255)
ggggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCg > 1:1036912/1‑100 (MQ=255)
ggAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCt > 1:954193/1‑100 (MQ=255)
gAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTa > 1:1291353/1‑100 (MQ=255)
aaGACGGTCAGTTTCTGCCGGAATTTTTGCGCATTTCGCCCAACATCAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAg < 2:2263723/100‑1 (MQ=255)
gCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAGCct > 1:1379800/1‑100 (MQ=255)
|
ATCTCTATTTCGCCCCGACACCCAATGGTCACAAAATTACGCTGTTTCTCGAAGAAGCAGAGCTGGATTATCGCTTGATTAAGGTAGACCTGGGGAAAGGCGGTCAGTTTCGTCCGGAATTTTTGCGCATTTCGCCCAACAACAAAATTCCGGCAATTGTTGATCATTCTCCTGCCGATGGCGGCGAACCGCTAAGCCT > NC_000913/2420628‑2420826
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
