Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F28 I1 R1 49 59.7 2689356 96.3% 2589849 141.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,986,841 T→A intergenic (+121/‑78) PP_5245 → / → kefB‑III AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,986,8410TA94.4% 227.7 / ‑2.0 71intergenic (+121/‑78)PP_5245/kefB‑IIIAraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (31/36);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGCGGTGAACAGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGT  >  NC_002947/5986697‑5986983
                                                                                                                                                  |                                                                                                                                               
ggtggCGGTGAACAGTCCGATTAGCGCGTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTATGGACAGGACAATTTTTCTTACCGTCAGATGATTGACt                                                                                                                                               >  1:188361/1‑148 (MQ=255)
  tggCGGTGAACAGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACg                                                                                                                                           >  2:146170/1‑149 (MQ=255)
        ttaaCATTCAGATTAGATC‑TGTCTTCTTCGCGTGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCTGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCcac                                                                                                                                       <  3:192316/145‑1 (MQ=255)
           aCAGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTAc                                                                                                                                  <  1:244227/149‑1 (MQ=255)
            cAGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAcc                                                                                                                                 <  1:42242/149‑1 (MQ=255)
             aGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAccc                                                                                                                                >  6:140576/1‑149 (MQ=255)
             aGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAccc                                                                                                                                <  3:306629/149‑1 (MQ=255)
             aGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAccc                                                                                                                                <  1:146170/149‑1 (MQ=255)
               tCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCt                                                                                                                              >  3:215449/1‑149 (MQ=255)
                 cGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa                                                                                                                            <  2:188361/149‑1 (MQ=255)
                         gc‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTc                                                                                                                   <  2:339385/149‑1 (MQ=255)
                          c‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTcc                                                                                                                  >  7:171764/1‑149 (MQ=255)
                             gCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGt                                                                                                                >  7:160539/1‑149 (MQ=255)
                              ccTTCTTCGCGGGCTCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCg                                                                                                                 <  4:88777/147‑1 (MQ=255)
                              ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGt                                                                                                                >  5:85299/1‑148 (MQ=255)
                                      gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCCCACTACCCCTCAG‑AATTGTCCGGCAAACTGtt                                                                                                       >  7:108885/1‑149 (MQ=255)
                                      gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGtt                                                                                                       <  2:1361/149‑1 (MQ=255)
                                      gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGtt                                                                                                       >  8:271764/1‑149 (MQ=255)
                                       cgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTc                                                                                                      <  8:266885/149‑1 (MQ=255)
                                       cgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTc                                                                                                      <  5:140576/149‑1 (MQ=255)
                                              gcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCGACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGc                                                                                                >  1:84145/1‑149 (MQ=255)
                                                      ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        <  8:160539/148‑1 (MQ=255)
                                                      ccACAGAGATAGCACAGGCCTCGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        <  4:247188/148‑1 (MQ=255)
                                                       cacaGCGATAGCACAGGCCTTGAGCCCAGGGAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCa                                                                                      <  8:248029/149‑1 (MQ=255)
                                                       cacaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        >  4:326635/1‑147 (MQ=255)
                                                         caGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACTCTGCCCCGGCAGc                                                                                    >  8:302092/1‑149 (MQ=255)
                                                              aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                               <  4:168109/149‑1 (MQ=255)
                                                               tAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                               <  7:302092/148‑1 (MQ=255)
                                                                 gCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAg                                                                            <  2:84145/149‑1 (MQ=255)
                                                                 gCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTCTCACTCTGGACACGACAATTTATCTACCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                               >  7:13518/1‑146 (MQ=255)
                                                                         cTTGAGCTCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACGATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        <  8:209859/129‑1 (MQ=255)
                                                                         cTTGAGCGCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACGATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        >  7:209859/1‑129 (MQ=255)
                                                                               cccAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                               >  1:235684/1‑148 (MQ=255)
                                                                               cccAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                               >  2:30669/1‑148 (MQ=255)
                                                                                 cAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcac                                                            <  3:326635/149‑1 (MQ=255)
                                                                                         cgcCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAgg                                                     >  3:118607/1‑148 (MQ=255)
                                                                                            cGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGTTGATTGACTGACGCCACTCTAGCCCTCAG‑TATTGTCCGTCAAACTGTTCGAACGCTGCGCCGGCAGCTCTTGGAGTAGGAGCCAATGCGACACGAAGGCAGc                                                 <  8:13518/149‑1 (MQ=255)
                                                                                               c‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt                                              >  1:27947/1‑149 (MQ=255)
                                                                                                   cGATTGTCACGCAGGCCAGGACAATTTTTCTGCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTGCGACCGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTg                                           <  8:108885/149‑1 (MQ=255)
                                                                                                            ctctGCACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAT‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa                                                                          <  8:79098/109‑1 (MQ=255)
                                                                                                               tGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCgg                                 <  4:118607/147‑1 (MQ=255)
                                                                                                                 gacaggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGt                                <  2:27947/146‑1 (MQ=255)
                                                                                                                   caggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                               <  8:205160/113‑1 (MQ=255)
                                                                                                                   caggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                               >  7:205160/1‑113 (MQ=255)
                                                                                                                   caggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg                                  >  5:77227/1‑142 (MQ=255)
                                                                                                                   caggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg                                  <  6:77227/142‑1 (MQ=255)
                                                                                                                    aggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCtt                          >  7:175825/1‑149 (MQ=255)
                                                                                                                     ggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc                         <  5:216752/149‑1 (MQ=255)
                                                                                                                     ggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc                         <  6:85299/149‑1 (MQ=255)
                                                                                                                      gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCt                                                                                               <  8:235885/78‑1 (MQ=255)
                                                                                                                      gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCt                                                                                               >  7:235885/1‑78 (MQ=255)
                                                                                                                      gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc                        >  8:314399/1‑149 (MQ=255)
                                                                                                                       acaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCt                       >  3:64766/1‑149 (MQ=255)
                                                                                                                         aaTTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                                        <  1:45173/83‑1 (MQ=255)
                                                                                                                         aaTTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc                     <  4:97677/149‑1 (MQ=255)
                                                                                                                         aaTTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc                     >  3:97677/1‑149 (MQ=255)
                                                                                                                           tttttCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt                   <  4:215449/149‑1 (MQ=255)
                                                                                                                           tttttCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGa                                                                       >  5:35208/1‑97 (MQ=255)
                                                                                                                           tttttCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGa                                                                       <  6:35208/97‑1 (MQ=255)
                                                                                                                              ttcttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCgg                                 <  2:305889/132‑1 (MQ=255)
                                                                                                                              ttcttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCgg                                 >  1:305889/1‑132 (MQ=255)
                                                                                                                                   cccGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCa                                                   <  5:33530/109‑1 (MQ=255)
                                                                                                                                   cccGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCa                                                   >  6:33530/1‑109 (MQ=255)
                                                                                                                                    ccGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTc          <  8:171764/149‑1 (MQ=255)
                                                                                                                                    ccGTCAGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGCTGATCTTCCTGCTTGCCGACGTc          >  8:155476/1‑149 (MQ=255)
                                                                                                                                         aGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCg      <  5:166005/148‑1 (MQ=255)
                                                                                                                                         aGATGATTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCg      >  6:166005/1‑148 (MQ=255)
                                                                                                                                          gatgatTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAgg                                                     >  2:28920/1‑100 (MQ=255)
                                                                                                                                          gatgatTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCCGCCCCGGCAGCTCATg                                                                               >  5:36647/1‑74 (MQ=255)
                                                                                                                                          gatgatTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCCGCCCCGGCAGCTCATg                                                                               <  6:36647/74‑1 (MQ=255)
                                                                                                                                            tgatTGACTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGt  <  7:314399/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGTGGCGGTGAACAGTCCGATTAGCGC‑TGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGC‑ACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAG‑AATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGT  >  NC_002947/5986697‑5986983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A