Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A15 F112 I0 R1
|
1434 |
61.8 |
2963302 |
95.2% |
2821063 |
140.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
5,986,841 |
T→A |
100% |
intergenic (+121/‑78) |
PP_5245 → / → kefB‑III |
AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 5,986,841 | 0 | T | A | 100.0%
| 153.4
/ NA
| 46 | intergenic (+121/‑78) | PP_5245/kefB‑III | AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (23/23); total (23/23) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG > NC_002947/5986697‑5986982
|
ggtggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACTAATTTTTCTTCCCGTCAGATGATTGACt < 1:21073/148‑1 (MQ=255)
ggtggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGa > 7:167499/1‑149 (MQ=255)
gTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCcaca > 1:160817/1‑149 (MQ=255)
gTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCcaca > 1:104038/1‑149 (MQ=255)
cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa < 2:78531/149‑1 (MQ=255)
cGATTAGCGCTGCCTTCTTCGCGGGCGAGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt > 8:320148/1‑147 (MQ=255)
aTTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACACCTCAg > 8:138329/1‑148 (MQ=255)
ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGaa < 2:96201/149‑1 (MQ=255)
gcgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCCCACTACCCCTCAGAATTg > 3:54249/1‑149 (MQ=255)
gcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGCGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTc < 2:213390/149‑1 (MQ=255)
cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa < 8:167499/148‑1 (MQ=255)
ttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa < 2:84876/132‑1 (MQ=255)
ttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa > 1:84876/1‑132 (MQ=255)
gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGtt < 2:81985/149‑1 (MQ=255)
gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACg < 2:104038/149‑1 (MQ=255)
cccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTTCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTg > 3:168402/1‑149 (MQ=255)
aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCTTCAGATGATTGACTGACGCCACACTTCCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg < 7:309733/149‑1 (MQ=255)
tAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg < 7:320148/148‑1 (MQ=255)
aGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa < 4:256100/149‑1 (MQ=255)
ggCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACACCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGa < 7:138329/149‑1 (MQ=255)
cTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa < 5:263351/140‑1 (MQ=255)
cTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa > 6:263351/1‑140 (MQ=255)
ccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa > 5:345740/1‑102 (MQ=255)
ccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa < 6:345740/102‑1 (MQ=255)
ggTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCacac < 3:69902/149‑1 (MQ=255)
gcgcCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAgg < 2:160817/149‑1 (MQ=255)
gCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg < 8:122471/108‑1 (MQ=255)
gCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg > 7:122471/1‑108 (MQ=255)
aCCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc < 2:94230/149‑1 (MQ=255)
tctGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCt < 8:175816/138‑1 (MQ=255)
tctGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCt > 7:175816/1‑138 (MQ=255)
gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg > 8:86857/1‑99 (MQ=255)
gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg < 7:86857/99‑1 (MQ=255)
gacaggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGAt > 5:163892/1‑149 (MQ=255)
aggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt > 3:17559/1‑50 (MQ=255)
aggac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt < 4:17559/50‑1 (MQ=255)
gac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc > 3:173666/1‑148 (MQ=255)
gac‑aATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc < 4:173666/148‑1 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGACTGCCGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc > 2:367024/1‑149 (MQ=255)
aTTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCt > 2:329106/1‑149 (MQ=255)
tttttCTTCCCGTCAGATGATTGACTGACGCCACACTACCACTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt > 5:149450/1‑149 (MQ=255)
ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc > 8:12624/1‑149 (MQ=255)
cccGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGt > 8:216678/1‑149 (MQ=255)
aGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt > 3:176655/1‑108 (MQ=255)
aGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCtt < 4:176655/108‑1 (MQ=255)
atgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg > 8:57676/1‑149 (MQ=255)
|
GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGAC‑AATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG > NC_002947/5986697‑5986982
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
