Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
A13 F103 I0 R1
|
1593 |
59.4 |
2889406 |
94.0% |
2716041 |
140.5 |
Breseq alignment
BRESEQ :: Evidence
|
evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
RA |
NC_002947 |
5,986,841 |
T→A |
13.2% |
intergenic (+121/‑78) |
PP_5245 → / → kefB‑III |
AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
|
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
* | NC_002947 | 5,986,841 | 0 | T | A | 13.2%
| 145.3
/ 11.2
| 60 | intergenic (+121/‑78) | PP_5245/kefB‑III | AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
Reads supporting (aligned to +/- strand): ref base T (25/25); new base A (6/2); total (32/28) |
Fisher's exact test for biased strand distribution p-value = 2.63e-01 |
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCT > NC_002947/5986703‑5986989
|
ggTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGACAATCATCTGACgggaa < 1:50118/149‑5 (MQ=255)
gAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTc > 1:292516/1‑137 (MQ=255)
gAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTc < 2:292516/137‑1 (MQ=255)
aCAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTAc < 8:140397/149‑1 (MQ=255)
cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCtta > 5:115686/1‑147 (MQ=255)
cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCtta < 2:262521/149‑3 (MQ=255)
gATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt > 5:99863/1‑146 (MQ=255)
ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGaa < 2:190991/149‑1 (MQ=255)
ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTc < 3:3762/149‑1 (MQ=255)
ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 7:64211/1‑148 (MQ=255)
ttcttcGCGGGCGAGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCtta > 6:124846/1‑133 (MQ=255)
ttcttcGCGGGCGAGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCtta < 5:124846/135‑3 (MQ=255)
tcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 1:281264/1‑148 (MQ=255)
gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 6:115686/149‑1 (MQ=255)
gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGAACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 7:193303/149‑1 (MQ=255)
ccGCTCCCACAGAGATATCACAGTACTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCAGTCAGATGATTGACTGACGACACACTAc < 4:236207/113‑1 (MQ=255)
ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc > 1:237828/1‑127 (MQ=255)
ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc < 2:237828/127‑1 (MQ=255)
ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATATTTCTTCCCGTCAGATGATTGACTGACGACACACTAc > 3:236207/1‑113 (MQ=255)
tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 8:52090/1‑149 (MQ=255)
tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 3:124148/1‑149 (MQ=255)
ccACAGAGATAGCACATGCCTTGAGCCCAGGTAAGC‑GCCGTCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg < 3:262419/147‑1 (MQ=255)
ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg < 4:166151/148‑1 (MQ=255)
ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGc > 2:112171/1‑149 (MQ=255)
acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg > 3:347603/1‑149 (MQ=255)
tAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATgg < 4:347603/149‑1 (MQ=255)
gCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa < 8:64211/148‑1 (MQ=255)
gCACAGGCCTTGACCCCAGGTAAGC‑GCCGGGACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTCCCCCGGCAGCTCATGGa < 3:277962/148‑1 (MQ=255)
cacaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGAc < 7:326586/85‑1 (MQ=255)
cacaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGAc > 2:340976/1‑85 (MQ=255)
cacaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGAc > 8:326586/1‑85 (MQ=255)
cacaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGAc < 1:340976/85‑1 (MQ=255)
acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa > 6:244363/1‑149 (MQ=255)
acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa < 2:217976/149‑1 (MQ=255)
gCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAg > 7:279848/1‑149 (MQ=255)
ttGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCACTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGcc > 6:201054/1‑148 (MQ=255)
aGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcaca > 3:69927/1‑149 (MQ=255)
aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc < 6:99863/149‑1 (MQ=255)
gATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTg > 2:89679/1‑148 (MQ=255)
aTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGc > 2:292793/1‑148 (MQ=255)
ttttaaCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGc < 7:340674/132‑1 (MQ=255)
ttGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACGGCAGATCATGGAGTACGAGCCAATGCAACACGAAGGc > 8:340674/1‑137 (MQ=255)
gTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCaaa < 5:244363/148‑1 (MQ=255)
ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 8:128448/1‑149 (MQ=255)
ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 1:309877/1‑149 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc > 5:164483/1‑149 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt < 7:236933/108‑1 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt > 8:236933/1‑108 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc > 6:265391/1‑145 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc < 5:265391/145‑1 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCATAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc > 5:168634/1‑149 (MQ=255)
tttttCTTCCGGTCTGATGATTGTCTGACGCCACTCTACCCCTTAGAATTGTCCGTCATACTGTTCGAAGGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt < 6:168634/149‑1 (MQ=255)
ttcCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACAGCGGTGATCTTCCTGCTTgccgcc > 6:325248/1‑149 (MQ=255)
ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc > 3:321719/1‑149 (MQ=255)
tCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTc < 2:309877/149‑1 (MQ=255)
aGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 8:279848/149‑1 (MQ=255)
aGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 1:292793/149‑1 (MQ=255)
gatgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGcc > 4:319994/1‑149 (MQ=255)
atgatTGTCTGACGCCACACTACCCCTTAGAATTGTACGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTATGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg > 7:228344/1‑149 (MQ=255)
ttGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACTGCAGCTCATGGAGTACGACCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTACTCCTTGACGCCGTCCTCGCAGTccc > 6:217862/1‑149 (MQ=255)
tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt > 8:285333/1‑149 (MQ=255)
|
GGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCT > NC_002947/5986703‑5986989
|
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A