Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,060	G→A	L34L (CTG→TTG)	rutC ←	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,060	0	G	A	100.0%	199.3 / NA	62	L34L (CTG→TTG)	rutC	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (35/27); total (35/27)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGT  >  NC_000913/1071929‑1072194

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 >  2:889704/1‑139 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 <  2:1265397/139‑1 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 >  1:1265397/1‑139 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 <  2:5302/139‑1 (MQ=255)

      gAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                           >  1:152842/1‑139 (MQ=255)

         ggTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 <  2:1302622/130‑1 (MQ=255)

         ggTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 >  1:1302622/1‑130 (MQ=255)

           tCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGGTATTATGTTGATCAAAAGCCAACGTACCGGAGACATacac                                                                                                                      >  1:581908/1‑139 (MQ=255)

                            ccACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 <  2:1432842/111‑1 (MQ=255)

                            ccACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 >  1:1432842/1‑111 (MQ=255)

                               cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGc                                                                                                  <  2:816918/139‑1 (MQ=255)

                                 cgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCg                                                                                                >  2:182462/1‑139 (MQ=255)

                                   ccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 <  1:1253526/104‑1 (MQ=255)

                                   ccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                                 >  2:1253526/1‑104 (MQ=255)

                                                cTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGcc                                                                                 >  1:552413/1‑139 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCa                                                                                                               >  2:1044872/1‑107 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCa                                                                                                               <  1:1044872/107‑1 (MQ=255)

                                                      gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                           >  2:1455055/1‑91 (MQ=255)

                                                      gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                           <  1:1455055/91‑1 (MQ=255)

                                                      gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                           <  1:182462/139‑1 (MQ=255)

                                                          gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTgcgc                                                                       >  1:1149444/1‑139 (MQ=255)

                                                             tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGga                                                                                                                               <  2:1034857/80‑1 (MQ=255)

                                                             tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCgatgc                                                                    >  2:584421/1‑139 (MQ=255)

                                                             tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAACGga                                                                                                                               >  1:1034857/1‑80 (MQ=255)

                                                                   aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGaa                                                                                            >  1:396004/1‑109 (MQ=255)

                                                                   aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGaa                                                                                            <  2:396004/109‑1 (MQ=255)

                                                                     gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                           <  2:831209/76‑1 (MQ=255)

                                                                     gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                           >  1:831209/1‑76 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg                                                          >  1:468208/1‑139 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg                                                          >  1:547933/1‑139 (MQ=255)

                                                                                       tGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc                                                             <  2:1119359/120‑1 (MQ=255)

                                                                                       tGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc                                                             >  1:1119359/1‑120 (MQ=255)

                                                                                              tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                                   <  2:656282/43‑1 (MQ=255)

                                                                                              tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                                   >  1:656282/1‑43 (MQ=255)

                                                                                              tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCg                                   >  1:258684/1‑139 (MQ=255)

                                                                                                   aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                              <  1:813510/139‑1 (MQ=255)

                                                                                                    aaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaat                                                       <  2:1320111/113‑1 (MQ=255)

                                                                                                    aaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGaat                                                       >  1:1320111/1‑113 (MQ=255)

                                                                                                        gCACGTTATTATGTTGATCAAAAGTCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCt                         <  2:902549/139‑1 (MQ=255)

                                                                                                                 tatGTTGATCAAAAGCCAACGTACCGGATACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATGATTACGGATTTTGGCATCGTTAACCTCCTTAAGCGata                <  2:581908/139‑1 (MQ=255)

                                                                                                                 tatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata                <  2:547933/139‑1 (MQ=255)

                                                                                                                   tGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGctgctg                                                                  >  1:788926/1‑87 (MQ=255)

                                                                                                                   tGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGctgctg                                                                  <  2:788926/87‑1 (MQ=255)

                                                                                                                       gATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa          <  2:1149444/139‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCa                                                                                                               >  2:1245674/1‑37 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCa                                                                                                               <  1:1245674/37‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGc                                                                                                  >  1:1582290/1‑50 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGc                                                                                                  <  2:1582290/50‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGcc                                                                <  2:757066/84‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGcc                                                                >  1:757066/1‑84 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata                >  1:121137/1‑132 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata                <  2:121137/132‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  2:331555/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  1:28988/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  2:1283086/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  2:820313/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  1:1053961/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATATTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa         >  2:971843/1‑139 (MQ=255)

                                                                                                                          cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                        <  1:733171/106‑1 (MQ=255)

                                                                                                                          cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                        >  2:733171/1‑106 (MQ=255)

                                                                                                                          cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAg       >  2:727062/1‑139 (MQ=255)

                                                                                                                               gCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGt  <  2:258684/139‑1 (MQ=255)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGT  >  NC_000913/1071929‑1072194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A