Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,072,060 |
G→A |
L34L (CTG→TTG) |
rutC ← |
putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,072,060 | 0 | G | A | 98.6%
| 233.4
/ NA
| 72 | L34L (CTG→TTG) | rutC | putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (36/35); minor base T (0/1); total (36/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAC > NC_000913/1071929‑1072192
|
gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCTTCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg > 2:969123/1‑139 (MQ=255)
gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg < 2:176147/139‑1 (MQ=255)
gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCCTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg > 2:1399286/1‑139 (MQ=255)
tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCTTCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGga < 1:969123/139‑1 (MQ=255)
ttGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGgaga < 2:491194/139‑1 (MQ=255)
tCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATacac > 1:1327985/1‑139 (MQ=255)
ccATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATc > 1:567236/1‑139 (MQ=255)
gCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc > 2:1540086/1‑139 (MQ=255)
cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCTGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCATCGTACCGGAGACAt < 2:350613/115‑1 (MQ=255)
cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg < 1:440016/108‑1 (MQ=255)
cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg > 2:440016/1‑108 (MQ=255)
cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACAt > 1:350613/1‑115 (MQ=255)
cgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCg > 1:1473156/1‑139 (MQ=255)
ccTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 1:710596/139‑1 (MQ=255)
ccTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 2:567236/139‑1 (MQ=255)
cTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGcc > 1:1006439/1‑139 (MQ=255)
tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg < 2:1007273/131‑1 (MQ=255)
tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg > 1:1007273/1‑131 (MQ=255)
cGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCg > 1:150372/1‑139 (MQ=255)
gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt < 1:1399286/139‑1 (MQ=255)
ttCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCgcta < 2:806338/139‑2 (MQ=255)
cGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc < 2:904050/139‑1 (MQ=255)
cGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc > 1:183025/1‑139 (MQ=255)
cGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc > 1:1562336/1‑139 (MQ=255)
gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 1:1488474/117‑1 (MQ=255)
gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc > 2:1488474/1‑117 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGa > 1:121170/1‑108 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGa < 2:121170/108‑1 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc > 1:1482264/1‑115 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 2:1482264/115‑1 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAg > 1:1197139/1‑118 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAg < 2:1197139/118‑1 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg > 2:214674/1‑139 (MQ=255)
tGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg < 2:692397/60‑1 (MQ=255)
tGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg > 1:692397/1‑60 (MQ=255)
tGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa < 2:1473156/139‑1 (MQ=255)
cgcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGTGAATAATTCCg < 2:1006439/139‑1 (MQ=255)
ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTg > 2:553074/1‑83 (MQ=255)
ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTg < 1:553074/83‑1 (MQ=255)
ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg < 2:1327985/139‑1 (MQ=255)
tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCAt < 2:507285/64‑1 (MQ=255)
tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCAt > 1:507285/1‑64 (MQ=255)
tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 2:308744/92‑1 (MQ=255)
tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc > 1:308744/1‑92 (MQ=255)
tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCg > 2:45301/1‑139 (MQ=255)
ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCg > 1:503653/1‑95 (MQ=255)
ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCg < 2:503653/95‑1 (MQ=255)
aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg > 2:294344/1‑111 (MQ=255)
aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg < 1:294344/111‑1 (MQ=255)
aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa < 2:150372/139‑1 (MQ=255)
ttatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGat < 2:1562336/139‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg < 2:1653607/61‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg > 1:1653607/1‑61 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt > 2:881251/1‑73 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt < 1:881251/73‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc > 1:229122/1‑87 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc < 2:907814/87‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc > 1:907814/1‑87 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc < 2:229122/87‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg < 2:536824/100‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg > 1:536824/1‑100 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGc > 2:387029/1‑128 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGc < 1:387029/128‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa > 2:439840/1‑139 (MQ=255)
cAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc > 2:689117/1‑44 (MQ=255)
cAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccagc < 1:689117/44‑1 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc > 1:795484/1‑61 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGc < 2:795484/61‑1 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa < 2:1393529/121‑1 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa > 1:1393529/1‑121 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAc > 1:846338/1‑139 (MQ=255)
aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAc < 1:1540086/139‑1 (MQ=255)
|
GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAC > NC_000913/1071929‑1072192
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
