Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,060	G→A	L34L (CTG→TTG)	rutC ←	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,060	0	G	A	100.0%	148.4 / NA	47	L34L (CTG→TTG)	rutC	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/24); total (23/24)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAA  >  NC_000913/1071929‑1072187

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  2:1673168/1‑139 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  2:1802246/1‑139 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  2:398918/139‑1 (MQ=255)

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  2:755717/139‑1 (MQ=255)

              caTCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  1:769410/125‑1 (MQ=255)

              caTCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  2:769410/1‑125 (MQ=255)

                    ccATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                            <  1:1168848/117‑1 (MQ=255)

                    ccATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                            >  2:1168848/1‑117 (MQ=255)

                            ccACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  2:1812770/1‑111 (MQ=255)

                            ccACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  1:1812770/111‑1 (MQ=255)

                                  gccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCgg                                                                                        <  2:260517/139‑1 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  2:1812755/89‑1 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  1:1812755/1‑89 (MQ=255)

                                                      gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                    <  2:1377363/139‑1 (MQ=255)

                                                      gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                    >  1:1377363/1‑139 (MQ=255)

                                                          gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg                                                                                <  1:472447/123‑1 (MQ=255)

                                                          gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAg                                                                                >  2:472447/1‑123 (MQ=255)

                                                             tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                             <  2:1427444/91‑1 (MQ=255)

                                                             tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                             >  1:1427444/1‑91 (MQ=255)

                                                               cAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGctgctg                                                           <  2:1732194/139‑1 (MQ=255)

                                                                      tGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCgg                                                    >  2:739946/1‑139 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACg                                                                                                                               <  2:685824/63‑1 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg                                                   >  1:342052/1‑139 (MQ=255)

                                                                                 cgcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  1:948966/58‑1 (MQ=255)

                                                                                 cgcCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  2:948966/1‑58 (MQ=255)

                                                                                      ttGGGTCATCGGCAAACAGCATGTTATTATGTTGATCAAAATCCAACGTACCGGAGACATACACCACGCCATCATCCAGCGTGGCGGGAACGAAGGGGGCCAGCGGTGCGCTGCGGCCAGCGGGAATAATTACGTAtgt                                    >  1:113658/1‑137 (MQ=255)

                                                                                      ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          <  2:146930/53‑1 (MQ=255)

                                                                                      ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                          >  1:146930/1‑53 (MQ=255)

                                                                                         ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                 <  1:1673168/139‑1 (MQ=255)

                                                                                                 gCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                         <  1:1802246/139‑1 (MQ=255)

                                                                                                                 tatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata         <  1:739946/139‑1 (MQ=255)

                                                                                                                   tGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                             >  1:1672514/1‑37 (MQ=255)

                                                                                                                   tGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                             <  2:1672514/37‑1 (MQ=255)

                                                                                                                     ttGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                               <  1:1110012/113‑1 (MQ=255)

                                                                                                                     ttGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                               >  2:1110012/1‑113 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                    >  2:1524707/1‑73 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                    <  1:1524707/73‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                        <  2:222089/85‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                        >  1:222089/1‑85 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa                                           <  2:217184/98‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa                                           >  1:217184/1‑98 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                       >  2:1017225/1‑118 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                       <  1:1017225/118‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                         >  2:1520748/1‑116 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                         <  1:1520748/116‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa  >  1:797204/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa  >  1:747843/1‑139 (MQ=255)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAA  >  NC_000913/1071929‑1072187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A