Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	780	68.3	3343050	93.5%	3125751	102.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,060	G→A	L34L (CTG→TTG)	rutC ←	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,060	0	G	A	100.0%	181.6 / NA	58	L34L (CTG→TTG)	rutC	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/28); total (30/28)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAA  >  NC_000913/1071929‑1072188

gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:1331925/1‑139 (MQ=255)

  tGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGga                                                                                                                          >  1:441613/1‑139 (MQ=255)

         ggTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATac                                                                                                                   >  1:856957/1‑139 (MQ=255)

           tCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATacac                                                                                                                 <  1:738779/139‑1 (MQ=255)

                 ccGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGcc                                                                                                           <  2:1331925/139‑1 (MQ=255)

                         gTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACAt                                                                                                                     <  2:1440382/121‑1 (MQ=255)

                         gTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACAt                                                                                                                     >  1:1440382/1‑121 (MQ=255)

                               cccGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGc                                                                                            >  1:755864/1‑139 (MQ=255)

                                    cgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                               >  2:774990/1‑116 (MQ=255)

                                    cgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                               <  1:774990/116‑1 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGc                                                                            >  2:582980/1‑136 (MQ=255)

                                                  tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGc                                                                            <  1:582980/136‑1 (MQ=255)

                                                         aGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTgcg                                                                  >  2:1058335/1‑139 (MQ=255)

                                                          gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:303540/1‑81 (MQ=255)

                                                          gTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                            <  2:303540/81‑1 (MQ=255)

                                                             tCCAGAACGT‑GCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  2:1492195/114‑1 (MQ=255)

                                                             tCCAGAACGT‑GCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  1:1492195/1‑114 (MQ=255)

                                                              ccAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  1:1490113/1‑114 (MQ=255)

                                                              ccAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  2:1490113/114‑1 (MQ=255)

                                                                   aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  1:953424/109‑1 (MQ=255)

                                                                   aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  2:953424/1‑109 (MQ=255)

                                                                     gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCg                                                      >  2:1435980/1‑139 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      >  2:827860/1‑105 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGaa                                                                                      <  1:827860/105‑1 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         >  2:330596/1‑118 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg                                                    <  1:1435980/139‑1 (MQ=255)

                                                                       ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACAGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         <  1:330596/118‑1 (MQ=255)

                                                                               tGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTa                                            >  2:1267137/1‑139 (MQ=255)

                                                                                    cTTTGGGTCATCGGCAAACAGCACGTTATTACGTTGATCAAAAGCCAACGTACCg                                                                                                                            >  1:972554/1‑55 (MQ=255)

                                                                                    cTTTGGGTCATCGGCAAACAGCACGTTATTACGTTGATCAAAAGCCAACGTACCg                                                                                                                            <  2:972554/55‑1 (MQ=255)

                                                                                      ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATc                                                                                                        <  2:1667755/73‑1 (MQ=255)

                                                                                      ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATc                                                                                                        >  1:1667755/1‑73 (MQ=255)

                                                                                         ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                  <  1:1267137/139‑1 (MQ=255)

                                                                                                  cAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaa                         <  2:856957/139‑1 (MQ=255)

                                                                                                   aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAT‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                        <  1:1058335/139‑1 (MQ=255)

                                                                                                         cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCTTCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTG‑CAGCGGGAATAAtt                                             <  1:126842/111‑1 (MQ=255)

                                                                                                         cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          >  1:961621/1‑131 (MQ=255)

                                                                                                         cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          <  2:961621/131‑1 (MQ=255)

                                                                                                         cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTG‑CAGCGGGAATAAtt                                             >  2:126842/1‑111 (MQ=255)

                                                                                                           cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         <  2:943364/82‑1 (MQ=255)

                                                                                                           cGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAg                                                                         >  1:943364/1‑82 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCTG‑CCAGCGTGcc                                                                                           <  2:789546/51‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGcc                                                                                           >  1:789546/1‑51 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAg                                                                                 >  2:1192064/1‑61 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAg                                                                                 <  1:1192064/61‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg                                          <  2:1665273/100‑1 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACg                                          >  1:1665273/1‑100 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa   >  1:780883/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa   >  2:42568/1‑139 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa    >  1:1136946/1‑138 (MQ=255)

                                                                                                                        aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCa    <  2:1136946/138‑1 (MQ=255)

                                                                                                                         tCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaaa  <  2:441613/139‑1 (MQ=255)

                                                                                                                          cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                         >  2:1241094/1‑84 (MQ=255)

                                                                                                                          cAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                         <  1:1241094/84‑1 (MQ=255)

                                                                                                                             aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                                <  1:107429/105‑1 (MQ=255)

                                                                                                                             aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCa                                >  2:107429/1‑105 (MQ=255)

                                                                                                                             aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          >  2:820713/1‑111 (MQ=255)

                                                                                                                             aaGCCAACGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                          <  1:820713/111‑1 (MQ=255)

GCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAG‑CCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAA  >  NC_000913/1071929‑1072188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A