Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,072,060 |
G→A |
L34L (CTG→TTG) |
rutC ← |
putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,072,060 | 0 | G | A | 100.0%
| 90.9
/ NA
| 29 | L34L (CTG→TTG) | rutC | putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/15); total (14/15) |
AGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATG > NC_000913/1071925‑1072182
|
aGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGACAAACAGCACATTATTATGTTGATCAAAAGCCAACGt > 2:534146/1‑139 (MQ=255)
gCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg > 2:95056/1‑139 (MQ=255)
cTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCgg < 1:361611/139‑1 (MQ=255)
cGTGCCACCCACCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagcc > 2:14864/1‑139 (MQ=255)
tGCCACCCACCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATcagccag < 1:14864/139‑1 (MQ=255)
gATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGa < 1:171398/137‑1 (MQ=255)
gATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGa > 2:171398/1‑137 (MQ=255)
cGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCg < 1:188376/139‑1 (MQ=255)
gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt < 1:95056/139‑1 (MQ=255)
aGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTACGTTGATCAAAAGCCAACGTACCGCAGACATACACCACGCTATCAGCCAGCCTGCCGGGAACGAAGGGGGCCAGCGGTGCGCtgctgc < 1:534146/139‑1 (MQ=255)
aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc > 1:452364/1‑70 (MQ=255)
aCGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc < 2:452364/70‑1 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg > 2:673019/1‑139 (MQ=255)
ggCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg < 1:673019/139‑1 (MQ=255)
ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAttt > 1:53942/1‑139 (MQ=255)
ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAttt > 1:229012/1‑139 (MQ=255)
gCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCt < 2:53942/139‑1 (MQ=255)
cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa > 1:571418/1‑40 (MQ=39)
cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa < 2:571418/40‑1 (MQ=255)
cACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCtt > 1:272912/1‑139 (MQ=255)
attatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg < 2:454574/99‑1 (MQ=255)
attatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg > 1:454574/1‑99 (MQ=255)
tatGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGata < 1:595621/139‑1 (MQ=255)
tGATCAAAAGCCAACGTACCGGAGACAGACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg > 2:500047/1‑92 (MQ=255)
cgATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCggg < 1:500047/91‑1 (MQ=255)
gATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATg > 2:397894/1‑135 (MQ=255)
gATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATg < 1:397894/135‑1 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTgcg > 1:754422/1‑76 (MQ=255)
aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTgcg < 2:754422/76‑1 (MQ=255)
|
AGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATG > NC_000913/1071925‑1072182
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
