Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,060	G→A	L34L (CTG→TTG)	rutC ←	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,060	0	G	A	100.0%	144.9 / NA	45	L34L (CTG→TTG)	rutC	putative aminoacrylate deaminase, reactive intermediate detoxification, weak enamine/imine deaminase activity
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/20); total (25/20)

ATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAC  >  NC_000913/1071927‑1072192

aTGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                                 <  2:1169960/139‑1 (MQ=255)

                       cATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATca                                                                                                          >  2:622128/1‑139 (MQ=255)

                                  ccgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCAGAGACATACACCACGCCATCAGCCAGCGTGcc                                                                                               <  2:782001/139‑1 (MQ=255)

                                   cgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                                 <  2:413885/104‑1 (MQ=255)

                                   cgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTAc                                                                                                                                 >  1:413885/1‑104 (MQ=255)

                                                        gATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                         >  1:863417/1‑139 (MQ=255)

                                                               tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCggg                                                                                            >  2:984808/1‑113 (MQ=255)

                                                               tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCggg                                                                                            <  1:984808/113‑1 (MQ=255)

                                                               tCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCgctgc                                                                  >  2:1004369/1‑139 (MQ=255)

                                                                   gAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGcc                                                              <  1:1004369/139‑1 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                               <  2:582951/70‑1 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCg                                                                                                                               >  1:582951/1‑70 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGgaga                                                                                                                           >  1:1037268/1‑74 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGgaga                                                                                                                           <  2:1037268/74‑1 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCtgctgc                                                               >  2:371901/1‑134 (MQ=255)

                                                                       gTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCtgctgc                                                               <  1:371901/134‑1 (MQ=255)

                                                                                        ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                                  <  2:1146007/66‑1 (MQ=255)

                                                                                        ttGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATAcacca                                                                                                                  >  1:1146007/1‑66 (MQ=255)

                                                                                           ggTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                      <  2:632653/139‑1 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCgg                                                                                                                              <  1:1135654/46‑1 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCgg                                                                                                                              >  2:1135654/1‑46 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                         >  1:279963/1‑51 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACa                                                                                                                         <  2:279963/51‑1 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                         <  1:884167/99‑1 (MQ=255)

                                                                                                tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGt                                                                         >  2:884167/1‑99 (MQ=255)

                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                      >  1:903213/1‑132 (MQ=255)

                                                                                                  ggCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTgg                                      <  2:903213/132‑1 (MQ=255)

                                                                                                   gCAAACAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTa                              <  1:124742/139‑1 (MQ=255)

                                                                                                     aaaCAGCACGTTATTATGTTGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTaaa                            <  1:622128/139‑1 (MQ=255)

                                                                                                                        tGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                             <  1:1120331/87‑1 (MQ=255)

                                                                                                                        tGATCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCa                                                             >  2:1120331/1‑87 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCAGGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:780436/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCtgctgc                                                               <  2:190669/83‑1 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCtgctgc                                                               >  1:190669/1‑83 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGTAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:791435/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAg                   <  2:924444/127‑1 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAg                   >  1:924444/1‑127 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:298669/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  1:111221/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:418551/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:332313/1‑139 (MQ=255)

                                                                                                                          aTCAAAAGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCaa       >  2:228915/1‑139 (MQ=255)

                                                                                                                               aaGCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAc  >  1:295768/1‑139 (MQ=255)

                                                                                                                                 gCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa                    <  2:408835/119‑1 (MQ=255)

                                                                                                                                 gCCAACGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTaa                    >  1:408835/1‑119 (MQ=255)

ATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGAC  >  NC_000913/1071927‑1072192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A