Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,581,621	G→A	A476A (GCC→GCT)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,621	0	G	A	100.0%	161.8 / NA	51	A476A (GCC→GCT)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/28); total (23/28)

TCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTG  >  NC_000913/4581487‑4581756

tcCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     <  2:1113311/139‑1 (MQ=255)

     cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     >  2:647844/1‑134 (MQ=255)

     cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     <  1:647844/134‑1 (MQ=255)

     cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                <  1:649118/139‑1 (MQ=255)

        cTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTg                                                                                                                             >  1:1084214/1‑139 (MQ=255)

          gACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTc                                                                                                                           >  2:1520673/1‑139 (MQ=255)

                  aaaTCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   >  1:892762/1‑139 (MQ=255)

                  aaaTCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   <  1:1520673/139‑1 (MQ=255)

                        gCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             <  1:142709/139‑1 (MQ=255)

                         ccTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                                  <  1:991956/133‑1 (MQ=255)

                         ccTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                                  >  2:991956/1‑133 (MQ=255)

                             aTCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGgag                                                                                                        >  2:1072434/1‑139 (MQ=255)

                               cgctcgcACCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTAAGCCAGGAGat                                                                                                      >  1:782041/1‑139 (MQ=255)

                                    gttcctaGTTAACGCATCTGCACATCCAGTTCAGACAGTTCCTGTACCAGTTCTCCCATCGCTTCTTCGGCTAATACATCCGTCTGCGGCAGGTTTTCAGCAACAATACTCCCTTTATCTTTCAGCCAGGAGATATCCa                                                                                                 <  1:308873/133‑1 (MQ=255)

                                       cccAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCttt                                                                                                                        <  1:1183450/113‑1 (MQ=255)

                                       cccAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCttt                                                                                                                        >  2:1183450/1‑113 (MQ=255)

                                          aGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   >  2:784295/1‑115 (MQ=255)

                                          aGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   <  1:784295/115‑1 (MQ=255)

                                                cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGAtt                                                                                     >  1:1178783/1‑139 (MQ=255)

                                                   aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                                  <  2:996018/139‑1 (MQ=255)

                                                        cgcATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             >  2:630426/1‑139 (MQ=255)

                                                        cgcATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             <  1:1072434/139‑1 (MQ=255)

                                                           aTCCAGTTCAGACAGCGCGTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtgcgg                                                                          <  2:1084214/139‑1 (MQ=255)

                                                           aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATACAGCGAATCGGATTTtgcgg                                                                               >  1:1479346/1‑134 (MQ=255)

                                                           aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATACAGCGAATCGGATTTtgcgg                                                                               <  2:1479346/134‑1 (MQ=255)

                                                             ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   >  1:129793/1‑96 (MQ=255)

                                                             ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   <  2:129793/96‑1 (MQ=255)

                                                                  tCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                                   >  2:499912/1‑139 (MQ=255)

                                                                        aGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGc                                                             <  1:1270515/139‑1 (MQ=255)

                                                                          cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             <  1:1464516/89‑1 (MQ=255)

                                                                          cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             >  2:1464516/1‑89 (MQ=255)

                                                                                tACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTg                                                     >  1:1207999/1‑139 (MQ=255)

                                                                                     gTTCGCCCATCGCTTCTGCCGCTAATCCATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             <  2:1219206/110‑1 (MQ=255)

                                                                                     gTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             >  1:1219206/1‑110 (MQ=255)

                                                                                      ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGAtt                                                                                     <  2:1423434/101‑1 (MQ=255)

                                                                                      ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGAtt                                                                                     >  1:1423434/1‑101 (MQ=255)

                                                                                      ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAg                                               <  1:470498/139‑1 (MQ=255)

                                                                                          cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                             >  2:36134/1‑137 (MQ=255)

                                                                                          cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                             <  1:36134/137‑1 (MQ=255)

                                                                                          cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                           <  2:1207999/139‑1 (MQ=255)

                                                                                          cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                           >  2:640788/1‑139 (MQ=255)

                                                                                           ccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCt                                          <  2:892762/139‑1 (MQ=255)

                                                                                                  ttCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCa                                   <  2:1392366/139‑1 (MQ=255)

                                                                                                               aCATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCgg                      <  2:655239/139‑1 (MQ=255)

                                                                                                                   ccggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGaa                                                                                            <  1:771130/65‑1 (MQ=255)

                                                                                                                   ccggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGaa                                                                                            >  2:771130/1‑65 (MQ=255)

                                                                                                                     ggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCTGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             <  1:1217937/78‑1 (MQ=255)

                                                                                                                     ggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                             >  2:1217937/1‑78 (MQ=255)

                                                                                                                            gcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTg              >  1:528636/1‑134 (MQ=255)

                                                                                                                            gcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTg              <  2:528636/134‑1 (MQ=255)

                                                                                                                                   gTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTg  <  1:640788/139‑1 (MQ=255)

TCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTG  >  NC_000913/4581487‑4581756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A