Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,581,621	G→A	A476A (GCC→GCT)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,621	0	G	A	100.0%	70.9 / NA	23	A476A (GCC→GCT)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/13); total (10/13)

TGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTG  >  NC_000913/4581496‑4581738

tGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGt                                                                                                          <  1:515361/139‑1 (MQ=255)

       gCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                   <  2:389695/123‑1 (MQ=255)

       gCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                   >  1:389695/1‑123 (MQ=255)

         aaaTCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                 <  2:36083/139‑1 (MQ=255)

               gCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                           <  1:239053/139‑1 (MQ=255)

               gCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                           <  1:388085/139‑1 (MQ=255)

                          cgcCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCCGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGgag                                                                                      >  1:230533/1‑133 (MQ=255)

                          cgcCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCCGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGgag                                                                                      <  2:230533/133‑1 (MQ=255)

                                       cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGAtt                                                                   >  1:781334/1‑139 (MQ=255)

                                          aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                <  2:722278/139‑1 (MQ=255)

                                            cAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcg                                                              <  2:781334/139‑1 (MQ=255)

                                                             aCAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACg                                             >  2:544637/1‑139 (MQ=255)

                                                              cAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACgg                                            <  1:470945/139‑1 (MQ=255)

                                                                             ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                           <  2:330353/77‑1 (MQ=255)

                                                                             ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                           >  1:330353/1‑77 (MQ=255)

                                                                                 cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                <  1:348787/100‑1 (MQ=255)

                                                                                 cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                >  2:348787/1‑100 (MQ=255)

                                                                                 cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAACATCAATACTGTCTTTATCTTTCAGcc                                                                                           <  2:22599/73‑1 (MQ=255)

                                                                                 cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAACATCAATACTGTCTTTATCTTTCAGcc                                                                                           >  1:22599/1‑73 (MQ=255)

                                                                                      cGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTa                    >  1:217435/1‑139 (MQ=255)

                                                                                                   aaTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGAt                                                      >  2:1016943/1‑92 (MQ=255)

                                                                                                   aaTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGAt                                                      <  1:1016943/92‑1 (MQ=255)

                                                                                                        aTCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGgtg  >  2:712755/1‑139 (MQ=255)

TGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTG  >  NC_000913/4581496‑4581738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A