Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,581,621	G→A	A476A (GCC→GCT)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,621	0	G	A	100.0%	134.8 / NA	42	A476A (GCC→GCT)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/24); total (18/24)

TCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTG  >  NC_000913/4581487‑4581756

tcCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     <  2:37160/139‑1 (MQ=255)

     cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                <  2:22229/139‑1 (MQ=255)

     cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                <  2:1101263/139‑1 (MQ=255)

        cTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTg                                                                                                                             <  2:1234673/139‑1 (MQ=255)

           aCGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                >  2:448648/1‑133 (MQ=255)

           aCGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                <  1:448648/133‑1 (MQ=255)

                gCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATc                                                                                                                     <  2:976235/139‑1 (MQ=255)

                  aaaTCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                                   <  1:333778/139‑1 (MQ=255)

                  aaaTCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAACAATACTGTCTTTATCtt                                                                                                                   <  2:697730/139‑1 (MQ=255)

                       ggCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGc                                                                                                              <  2:836109/139‑1 (MQ=255)

                             aTCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCTCTAATCCATCTTTCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGgag                                                                                                        <  2:621391/139‑1 (MQ=255)

                                         cAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGaa                                                                                            >  2:725/1‑139 (MQ=255)

                                                cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             >  2:1311454/1‑115 (MQ=255)

                                                cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             <  1:1311454/115‑1 (MQ=255)

                                                   aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                                  <  1:1280459/139‑1 (MQ=255)

                                                   aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                                  >  1:1136766/1‑139 (MQ=255)

                                                    tCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAg                                                                                                               >  1:873223/1‑109 (MQ=255)

                                                    tCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAg                                                                                                               <  2:873223/109‑1 (MQ=255)

                                                      aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             >  2:1052383/1‑109 (MQ=255)

                                                      aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                             <  1:1052383/109‑1 (MQ=255)

                                                           aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                >  1:154156/1‑85 (MQ=255)

                                                           aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                                <  2:154156/85‑1 (MQ=255)

                                                                      aCAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTc                                                                  <  1:245587/136‑1 (MQ=255)

                                                                      aCAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTc                                                                  >  2:245587/1‑136 (MQ=255)

                                                                         gcgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCt                                                            >  2:1153848/1‑139 (MQ=255)

                                                                          cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATc                                                                                          >  2:452824/1‑108 (MQ=255)

                                                                          cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATc                                                                                          <  1:452824/108‑1 (MQ=255)

                                                                                tACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     <  2:481201/59‑1 (MQ=255)

                                                                                tACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                                     >  1:481201/1‑59 (MQ=255)

                                                                                     gTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGc                                                             <  1:1010534/126‑1 (MQ=255)

                                                                                     gTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGc                                                             >  2:1010534/1‑126 (MQ=255)

                                                                                       tCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGc                                              >  2:210165/1‑139 (MQ=255)

                                                                                        cGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                             <  2:1136766/139‑1 (MQ=255)

                                                                                          cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                           >  2:1179534/1‑139 (MQ=255)

                                                                                             aTCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTgg                                        >  1:1041868/1‑139 (MQ=255)

                                                                                              tCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGt                                       >  1:180020/1‑139 (MQ=255)

                                                                                                           tAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                           >  2:228382/1‑122 (MQ=255)

                                                                                                           tAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                           <  1:228382/122‑1 (MQ=255)

                                                                                                             aTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTc                        <  1:793198/139‑1 (MQ=255)

                                                                                                                       ctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCt                                          >  1:317148/1‑111 (MQ=255)

                                                                                                                       ctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCt                                          <  2:317148/111‑1 (MQ=255)

                                                                                                                                   gTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTg  <  2:180020/139‑1 (MQ=255)

TCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCTCTTCGCTG  >  NC_000913/4581487‑4581756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A