Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,581,621	G→A	A476A (GCC→GCT)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,621	0	G	A	100.0%	137.0 / NA	44	A476A (GCC→GCT)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/22); total (22/22)

CAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCT  >  NC_000913/4581492‑4581748

cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                        <  1:1288632/139‑1 (MQ=255)

                   gCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCTGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                     <  2:920855/139‑1 (MQ=255)

                   gCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGTCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                     <  1:1011842/139‑1 (MQ=255)

                                    cAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAg                                                                                                   >  2:170743/1‑124 (MQ=255)

                                    cAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAg                                                                                                   <  1:170743/124‑1 (MQ=255)

                                      gTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATc                                                                                  >  2:454939/1‑139 (MQ=255)

                                           cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  <  1:1190178/118‑1 (MQ=255)

                                           cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  >  2:1190178/1‑118 (MQ=255)

                                              aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACACCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                          <  1:303782/139‑1 (MQ=255)

                                                 aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcgg                                                                       >  2:200872/1‑139 (MQ=255)

                                                      aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                          >  1:1245266/1‑99 (MQ=255)

                                                      aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                          <  2:1245266/99‑1 (MQ=255)

                                                        ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATAc                                                                                                                       >  2:868591/1‑84 (MQ=255)

                                                        ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATAc                                                                                                                       <  1:868591/84‑1 (MQ=255)

                                                           gTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTGCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGCTTTTGCGGTGCGGATCCa                                                             >  2:807731/1‑139 (MQ=255)

                                                             tCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                           <  1:200872/139‑1 (MQ=255)

                                                                     cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  <  1:1317750/92‑1 (MQ=255)

                                                                     cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  >  2:1317750/1‑92 (MQ=255)

                                                                     cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTg                                                   >  2:332308/1‑139 (MQ=255)

                                                                                gTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCa                                                                                                                              >  1:307562/1‑53 (MQ=255)

                                                                                gTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCa                                                                                                                              <  2:307562/53‑1 (MQ=255)

                                                                                 ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                     >  1:500284/1‑109 (MQ=255)

                                                                                 ttCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcggtg                                                                     <  2:500284/109‑1 (MQ=255)

                                                                                  tCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                           <  1:110379/118‑1 (MQ=255)

                                                                                  tCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                           >  2:110379/1‑118 (MQ=255)

                                                                                     cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  <  1:147029/76‑1 (MQ=255)

                                                                                     cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAgg                                                                                                  >  2:147029/1‑76 (MQ=255)

                                                                                     cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                   >  2:1016417/1‑139 (MQ=255)

                                                                                               cTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAg                         >  2:1264581/1‑139 (MQ=255)

                                                                                                    gCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGTATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCt                    <  1:807731/139‑1 (MQ=255)

                                                                                                     cTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                           <  1:908802/51‑1 (MQ=255)

                                                                                                     cTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                           >  2:908802/1‑51 (MQ=255)

                                                                                                       aaTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGt                 <  1:1294792/139‑1 (MQ=255)

                                                                                                       aaTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGt                 <  1:737786/139‑1 (MQ=255)

                                                                                                          aCATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCgg              <  1:454939/139‑1 (MQ=255)

                                                                                                             tccggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                           <  1:172312/43‑1 (MQ=255)

                                                                                                             tccggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                           >  2:172312/1‑43 (MQ=255)

                                                                                                               cggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGttt         >  1:216931/1‑139 (MQ=255)

                                                                                                                ggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATc                                                                                  <  1:7623/65‑1 (MQ=255)

                                                                                                                ggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATc                                                                                  >  2:7623/1‑65 (MQ=255)

                                                                                                                  ctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTg      >  1:1025566/1‑139 (MQ=255)

                                                                                                                      ggcAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                          <  2:871549/35‑1 (MQ=255)

                                                                                                                      ggcAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                          >  1:871549/1‑35 (MQ=255)

                                                                                                                      ggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTtct  >  1:1069586/1‑139 (MQ=255)

CAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTCT  >  NC_000913/4581492‑4581748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A