Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I2 R1 760 52.7 2744488 90.2% 2475528 105.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,581,621 G→A A476A (GCC→GCT)  hsdM ← DNA methyltransferase M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,581,6210GA100.0% 220.4 / NA 68A476A (GCC→GCT) hsdMDNA methyltransferase M
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/37);  total (31/37)

TTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTC  >  NC_000913/4581486‑4581747
                                                                                                                                       |                                                                                                                              
ttcCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCa                                                                                                                             <  2:1352458/139‑1 (MQ=255)
 tcCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                            <  1:567054/139‑1 (MQ=255)
     gCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAAt                                                                                                                        <  2:409086/139‑1 (MQ=255)
      cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:1003640/139‑1 (MQ=255)
      cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:742603/139‑1 (MQ=255)
      cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  2:698612/139‑1 (MQ=255)
      cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:1201915/139‑1 (MQ=255)
      cAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:1239807/139‑1 (MQ=255)
                          ccTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCa                                                                                                   >  1:1336640/1‑139 (MQ=255)
                           cTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAg                                                                                                  <  2:74448/139‑1 (MQ=255)
                           cTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAg                                                                                                  <  1:1303908/139‑1 (MQ=255)
                            tCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       >  1:890913/1‑117 (MQ=255)
                            tCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  2:890913/117‑1 (MQ=255)
                            tCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGt                                                                                                                   >  1:292308/1‑121 (MQ=255)
                            tCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGt                                                                                                                   <  2:292308/121‑1 (MQ=255)
                                    cgcCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATcc                                                                                         >  2:563713/1‑139 (MQ=255)
                                                 cGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGAtt                                                                            >  1:580825/1‑139 (MQ=255)
                                                    aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                         <  1:188670/139‑1 (MQ=255)
                                                    aTCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtg                                                                         <  2:950880/139‑1 (MQ=255)
                                                       aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       >  2:864650/1‑90 (MQ=255)
                                                       aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:864650/90‑1 (MQ=255)
                                                       aGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcgg                                                                      >  2:661573/1‑139 (MQ=255)
                                                            aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTc                                                                                                        <  2:315510/100‑1 (MQ=255)
                                                            aTCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTc                                                                                                        >  1:315510/1‑100 (MQ=255)
                                                              ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                          <  2:897759/96‑1 (MQ=255)
                                                              ccAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCtt                                                                                                          >  1:897759/1‑96 (MQ=255)
                                                                   tCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCaa                                                                                                                         >  1:87306/1‑76 (MQ=255)
                                                                   tCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCaa                                                                                                                         <  2:87306/76‑1 (MQ=255)
                                                                           cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       >  1:1042688/1‑70 (MQ=255)
                                                                           cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  2:1042688/70‑1 (MQ=255)
                                                                           cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTg                                                  >  1:956309/1‑139 (MQ=255)
                                                                           cgcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTg                                                  >  2:1031426/1‑139 (MQ=255)
                                                                            gcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                            >  1:113204/1‑64 (MQ=255)
                                                                            gcCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCAt                                                                                                                            <  2:113204/64‑1 (MQ=255)
                                                                                    cAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTgcgc                                         <  2:1336640/139‑1 (MQ=255)
                                                                                     aGTTCGCCCATCGCTTCTGCCGCTAATAC‑‑CCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAg                                      >  2:683228/1‑139 (MQ=255)
                                                                                         cGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                    >  1:173682/1‑139 (MQ=255)
                                                                                         cGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                    <  2:848239/139‑1 (MQ=255)
                                                                                         cGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCg                                    >  2:803342/1‑139 (MQ=255)
                                                                                          gCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCgg                                   <  2:1283858/139‑1 (MQ=255)
                                                                                          gCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCgg                                   <  1:314007/139‑1 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       <  1:507554/54‑1 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATa                                                                                                                       >  2:507554/1‑54 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                    <  2:925810/73‑1 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                  >  1:135189/1‑139 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                  <  2:748606/139‑1 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGc                                  >  1:446409/1‑139 (MQ=255)
                                                                                           cccATCGCTTCTGCCGCTAATACAGCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGcc                                                                                                    >  1:925810/1‑73 (MQ=255)
                                                                                              aTCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGAt                                                               >  1:787220/1‑107 (MQ=255)
                                                                                              aTCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGAt                                                               <  2:787220/107‑1 (MQ=255)
                                                                                              aTCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTgg                               <  2:173682/139‑1 (MQ=255)
                                                                                                           cTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcg                                                                       >  2:751872/1‑86 (MQ=255)
                                                                                                           cTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcg                                                                       <  1:751872/86‑1 (MQ=255)
                                                                                                           cTAATACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTg                  >  2:448761/1‑139 (MQ=255)
                                                                                                             aaTACATCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGt                <  1:661573/139‑1 (MQ=255)
                                                                                                                  aTCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                         <  1:354806/45‑1 (MQ=255)
                                                                                                                  aTCCGGCTCCGGCAGGCTGTCAGCATCAATACTGTCTTTATCttt                                                                                                         >  2:354806/1‑45 (MQ=255)
                                                                                                                    ccggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                          >  1:1191778/1‑90 (MQ=255)
                                                                                                                    ccggctcaggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                          <  2:1191778/90‑1 (MQ=255)
                                                                                                                     cggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                          >  2:7614/1‑89 (MQ=255)
                                                                                                                     cggctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACt                                                          <  1:7614/89‑1 (MQ=255)
                                                                                                                        ctccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTg     >  2:744891/1‑139 (MQ=255)
                                                                                                                          ccggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGtt   <  2:446409/139‑1 (MQ=255)
                                                                                                                           cggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTtc  >  2:75309/1‑139 (MQ=255)
                                                                                                                            ggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGtt   >  2:463524/1‑137 (MQ=255)
                                                                                                                            ggcAGGCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGtt   <  1:463524/137‑1 (MQ=255)
                                                                                                                                 gCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcgg                                                                      <  1:631791/65‑1 (MQ=255)
                                                                                                                                 gCTGTCAGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTtgcgg                                                                      >  2:631791/1‑65 (MQ=255)
                                                                                                                                       |                                                                                                                              
TTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTTCAGACAGCGCCTGTACCAGTTCGCCCATCGCTTCTGCCGCTAATACATCCGGCTCCGGCAGGCTGTCGGCATCAATACTGTCTTTATCTTTCAGCCAGGAGATATCCAGCGAATCGGATTTTGCGGTGCGGATCCACTCACGGCTGAACTTGCGCCAGCGGCTGGTAGCAAGATGCTGGTCGGTGTTTTTGTTC  >  NC_000913/4581486‑4581747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A