Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,478	(G)_7→8	coding (405/1113 nt)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,471	1	.	G	100.0%	113.9 / NA	32	M133R (ATG→AGG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/18); total (14/18)

GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTT  >  NC_000913/1769336‑1769591

gTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑ggg                                                                                                                       >  1:1264427/1‑139 (MQ=255)

gTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑ggg                                                                                                                       >  2:443522/1‑139 (MQ=255)

           ggCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCg                                                                                                             <  2:1264427/139‑1 (MQ=255)

             cAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAATTTGCTGGATATGGGGGGGGACGGCGAt                                                                                                           >  1:79143/1‑139 (MQ=255)

                cGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑ggggggg                                                                                                                   >  2:98570/1‑127 (MQ=255)

                cGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑ggggggg                                                                                                                   <  1:98570/127‑1 (MQ=255)

                    ccGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAATTTGCTGGATATGGGGGGGGACGGCGATCATGGCg                                                                                                    <  2:79143/139‑1 (MQ=255)

                          aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                              <  2:254449/139‑1 (MQ=255)

                          aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                              <  2:111677/139‑1 (MQ=255)

                                  cATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCATAATACCATACCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTta                                                                                      >  2:880570/1‑139 (MQ=255)

                                    tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTtata                                                                                    >  2:301513/1‑139 (MQ=255)

                                           cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCg                                                                                                    <  2:573003/116‑1 (MQ=255)

                                           cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCg                                                                                                    >  1:573003/1‑116 (MQ=255)

                                                        ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                         <  2:1147193/130‑1 (MQ=255)

                                                        ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                         >  1:1147193/1‑130 (MQ=255)

                                                        ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑gggg                                                                                                                      >  1:176306/1‑84 (MQ=255)

                                                        ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑gggg                                                                                                                      <  2:176306/84‑1 (MQ=255)

                                                           cccGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTc                                                             >  2:725167/1‑139 (MQ=255)

                                                                 cTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                         <  2:535327/121‑1 (MQ=255)

                                                                 cTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                         >  1:535327/1‑121 (MQ=255)

                                                                    gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg                                                    <  1:301513/139‑1 (MQ=255)

                                                                          cTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGgcggcg                                              <  2:1179585/139‑1 (MQ=255)

                                                                              aTACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCata                                          >  1:765304/1‑139 (MQ=255)

                                                                                    ttCCGGTGATTGGCGCGTAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                    >  2:268427/1‑139 (MQ=255)

                                                                                    ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAgg                                                   >  1:560336/1‑124 (MQ=255)

                                                                                    ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAgg                                                   <  2:560336/124‑1 (MQ=255)

                                                                                    ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                    >  1:1032191/1‑139 (MQ=255)

                                                                                       cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                              <  1:580145/78‑1 (MQ=255)

                                                                                       cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTc                                                                                              >  2:580145/1‑78 (MQ=255)

                                                                                       cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                 <  2:980792/139‑1 (MQ=255)

                                                                                       cGGTGATTGGCGCGAAGATGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGTGATCATGGCGAAAGTCCGCCCTTATATTGGCACCAGCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                 <  1:880570/139‑1 (MQ=255)

                                                                                               ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATc                                                                                                          <  1:1111598/58‑1 (MQ=255)

                                                                                               ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATc                                                                                                          >  2:1111598/1‑58 (MQ=255)

                                                                                               ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCttt                               <  2:837655/133‑1 (MQ=255)

                                                                                               ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCttt                               >  1:837655/1‑133 (MQ=255)

                                                                                                      aGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATtgtg                  <  2:765304/139‑1 (MQ=255)

                                                                                                                 ggCTGGCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt       <  1:268427/139‑1 (MQ=255)

                                                                                                                      gCACAACTTGCTGGATATGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCtt  <  2:1032191/139‑1 (MQ=255)

GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTT  >  NC_000913/1769336‑1769591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A