Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,769,478 (G)7→8 coding (405/1113 nt) ydiK → UPF0118 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,769,4711.G100.0% 147.4 / NA 43M133R (ATG→AGG) ydiKUPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/25);  total (18/25)

GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTG  >  NC_000913/1769336‑1769596
                                                                                                                                        |                                                                                                                               
gTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑ggg                                                                                                                            >  2:858915/1‑139 (MQ=255)
   tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggg                                                                                                                         <  1:1588671/139‑1 (MQ=255)
      tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggggga                                                                                                                      >  2:1727158/1‑137 (MQ=255)
      tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggggggga                                                                                                                      >  1:148558/1‑137 (MQ=255)
                         aaTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    <  2:152616/139‑1 (MQ=255)
                          aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:1054687/139‑1 (MQ=255)
                           ttAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  <  1:906414/139‑1 (MQ=255)
                                    tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTtata                                                                                         >  2:134469/1‑139 (MQ=255)
                                    tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggg                                                                                                                           >  1:1491636/1‑104 (MQ=255)
                                    tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑gggg                                                                                                                           <  2:1491636/104‑1 (MQ=255)
                                        cAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     <  1:293335/139‑1 (MQ=255)
                                                aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac                                                                                  <  1:1660950/134‑1 (MQ=255)
                                                aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac                                                                                  >  2:1660950/1‑134 (MQ=255)
                                                          aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCggg                                                                     >  1:1781028/1‑139 (MQ=255)
                                                          aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                       <  2:1273432/120‑1 (MQ=255)
                                                          aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt                                                                                       >  1:1273432/1‑120 (MQ=255)
                                                          aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                   >  2:492499/1‑139 (MQ=255)
                                                                    gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg                                                         <  1:492499/139‑1 (MQ=255)
                                                                    gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg                                                         <  1:499732/139‑1 (MQ=255)
                                                                    gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATACG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCGCCTGGTTCGTTGGGCAg                                                         <  1:1050072/139‑1 (MQ=255)
                                                                         gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                 >  1:665001/1‑94 (MQ=255)
                                                                         gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                 <  2:665001/94‑1 (MQ=255)
                                                                            tAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCa                                                 >  2:745752/1‑139 (MQ=255)
                                                                                   aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  <  1:1085620/83‑1 (MQ=255)
                                                                                   aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTcc                                                                                                  >  2:1085620/1‑83 (MQ=255)
                                                                                   aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCggg                                          <  1:858915/139‑1 (MQ=255)
                                                                                    ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     <  2:707625/95‑1 (MQ=255)
                                                                                    ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                     >  1:707625/1‑95 (MQ=255)
                                                                                       cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:1727158/139‑1 (MQ=255)
                                                                                       cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:134469/139‑1 (MQ=255)
                                                                                         gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      <  1:1333118/137‑1 (MQ=255)
                                                                                         gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                      >  2:1333118/1‑137 (MQ=255)
                                                                                           gATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTAt                                  <  2:148558/139‑1 (MQ=255)
                                                                                            aTTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATg                                 >  2:593518/1‑139 (MQ=255)
                                                                                                  gcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCTCCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCTCTTTATGGTGCAt                           <  1:168001/139‑1 (MQ=255)
                                                                                                           tATGCAGGCTGGCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTg                  >  2:556972/1‑139 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    <  1:1137506/47‑1 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGt                                                                                                    >  2:1137506/1‑47 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   >  2:609619/1‑48 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   >  2:670872/1‑48 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:609619/48‑1 (MQ=39)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                   <  1:670872/48‑1 (MQ=39)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccaccac                                                                         >  2:274646/1‑74 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccaccac                                                                         <  1:274646/74‑1 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                        >  2:1024466/1‑107 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg                                        <  1:1024466/107‑1 (MQ=255)
                                                                                                                     ggCACAACTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct        >  2:509983/1‑139 (MQ=255)
                                                                                                                           aCTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg  <  1:556972/139‑1 (MQ=255)
                                                                                                                           aCTTGCTGGATATG‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg  <  1:593518/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                               
GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTG  >  NC_000913/1769336‑1769596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A