Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,769,478 |
(G)7→8 |
coding (405/1113 nt) |
ydiK → |
UPF0118 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,769,471 | 1 | . | G | 100.0%
| 149.4
/ NA
| 41 | M133R (ATG→AGG) | ydiK | UPF0118 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (19/22); total (19/22) |
TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGC > NC_000913/1769339‑1769602
|
tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑gggggg > 2:514193/1‑139 (MQ=255)
gtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑gggggggg < 2:69144/139‑2 (MQ=255)
ggCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCg > 2:260335/1‑139 (MQ=255)
aGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATc < 1:40465/139‑1 (MQ=255)
aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTc < 2:259206/139‑1 (MQ=255)
aGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGccc < 1:43175/139‑1 (MQ=255)
cATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCtt > 2:812236/1‑139 (MQ=255)
tttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTtata > 2:144170/1‑139 (MQ=255)
cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac < 1:260335/139‑1 (MQ=255)
cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac < 2:387041/139‑1 (MQ=255)
cGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcac > 1:268171/1‑139 (MQ=255)
aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacca < 1:292814/139‑1 (MQ=255)
aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCa > 2:887672/1‑91 (MQ=255)
aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCa < 1:887672/91‑1 (MQ=255)
gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTc < 2:775097/92‑1 (MQ=255)
gCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTc > 1:775097/1‑92 (MQ=255)
cTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATACTGGCACCACCACCACCTGGTTCGTTGGGCAgg < 1:443689/134‑1 (MQ=255)
cTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATACTGGCACCACCACCACCTGGTTCGTTGGGCAgg > 2:443689/1‑134 (MQ=255)
cATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg < 2:565603/139‑1 (MQ=255)
ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc > 2:425898/1‑139 (MQ=255)
cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt < 1:144170/139‑1 (MQ=255)
gcgcGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt > 1:536147/1‑81 (MQ=255)
gcgcGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt < 2:536147/81‑1 (MQ=255)
gcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCa < 1:812236/139‑1 (MQ=255)
gCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATg < 1:302519/139‑1 (MQ=255)
aGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg < 1:356466/112‑1 (MQ=255)
aGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg > 2:356466/1‑112 (MQ=255)
aGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCt > 1:266144/1‑139 (MQ=255)
ggCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt < 2:64598/139‑1 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt < 2:382084/57‑1 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt > 1:382084/1‑57 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt < 1:728334/63‑1 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtt > 2:728334/1‑63 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtc > 2:127383/1‑139 (MQ=255)
ggCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct > 1:261445/1‑139 (MQ=255)
cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCa < 2:956085/87‑1 (MQ=255)
cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCa > 1:956085/1‑87 (MQ=255)
cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGa < 1:287601/128‑1 (MQ=255)
cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGa > 2:287601/1‑128 (MQ=255)
cTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc > 2:35104/1‑139 (MQ=255)
cTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt < 2:580241/124‑1 (MQ=255)
cTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt > 1:580241/1‑124 (MQ=255)
tGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCctgc < 2:930625/139‑1 (MQ=255)
|
TCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGC > NC_000913/1769339‑1769602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
