Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,478	(G)_7→8	coding (405/1113 nt)	ydiK →	UPF0118 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,471	1	.	G	100.0%	110.8 / NA	31	M133R (ATG→AGG)	ydiK	UPF0118 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/17); total (14/17)

CGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTG  >  NC_000913/1769340‑1769601

cgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑ggggggg                                                                                                                             >  2:717799/1‑139 (MQ=255)

     aCGGCAGCGGCCCGCGAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑ggggggggacgg                                                                                                                        >  1:537184/1‑134 (MQ=255)

               cccGCCAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGc                                                                                                               <  1:12142/139‑1 (MQ=255)

                      aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACCTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  1:85625/139‑1 (MQ=255)

                              cATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCtt                                                                                                 <  1:449064/139‑1 (MQ=255)

                                   ccAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATAtta                                                                                           <  1:280741/139‑2 (MQ=255)

                                           gACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                                   <  1:717799/139‑1 (MQ=255)

                                                    ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                      >  2:624600/1‑111 (MQ=255)

                                                    ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCg                                                                                                      <  1:624600/111‑1 (MQ=255)

                                                      aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt                                                                        >  1:623449/1‑139 (MQ=255)

                                                           aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTg                                                                   >  1:802573/1‑139 (MQ=255)

                                                            tCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTgg                                                                  <  2:802573/139‑1 (MQ=255)

                                                             cTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt                                                                                                  <  1:430549/106‑1 (MQ=255)

                                                             cTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCt                                                                                                  >  2:430549/1‑106 (MQ=255)

                                                                 cGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAgg                                                             <  2:145514/139‑1 (MQ=255)

                                                                      cTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGgcggcg                                                        >  1:521795/1‑139 (MQ=255)

                                                                          aTACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCata                                                    >  2:457166/1‑139 (MQ=255)

                                                                                ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                              >  1:399670/1‑139 (MQ=255)

                                                                                ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                              >  1:590388/1‑139 (MQ=255)

                                                                                ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                              >  2:647814/1‑139 (MQ=255)

                                                                                ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgc                                              >  2:459376/1‑139 (MQ=255)

                                                                                   cGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGACCATGGCGAAAGTCCGCCCTTATATTGGCGCCACCACCACCTGGTGCGTTGGGCAGGCGGCGCATATCGGGCGCt                                           <  2:537184/139‑1 (MQ=255)

                                                                                     gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCttt                                         <  2:590388/139‑1 (MQ=255)

                                                                                          tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt                                    <  2:237018/139‑1 (MQ=255)

                                                                                           ggCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCGGGCTCGTTGGGCAGGCCGCGCATCGCGGGCGCTTTATGGta                                   >  1:93454/1‑138 (MQ=255)

                                                                                             cgcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGAGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCa                                 <  1:493159/139‑1 (MQ=255)

                                                                                                  aGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATtgtg                            >  1:352609/1‑139 (MQ=255)

                                                                                                   gCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTgc                           <  1:217449/139‑1 (MQ=255)

                                                                                                                   cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTc                                                                       <  2:297855/79‑1 (MQ=255)

                                                                                                                   cacaACTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTc                                                                       >  1:297855/1‑79 (MQ=255)

                                                                                                                       aCTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg       <  2:399670/139‑1 (MQ=255)

                                                                                                                        cTTGCTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc      >  1:380539/1‑139 (MQ=255)

                                                                                                                            cTGGATATG‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCctg  <  2:623449/139‑1 (MQ=255)

CGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTG  >  NC_000913/1769340‑1769601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A