Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,769,478 |
(G)7→8 |
coding (405/1113 nt) |
ydiK → |
UPF0118 family inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,769,471 | 1 | . | G | 100.0%
| 77.9
/ NA
| 23 | M133R (ATG→AGG) | ydiK | UPF0118 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (12/11); total (12/11) |
CGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC > NC_000913/1769357‑1769599
|
cGCTAATTAAAGCCATTTCCAGCGGTGACATGAGGTTAACCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGACGCGCAGCGGTCTGCAGGCTGGCACGACATACTGGATAT‑‑GGGGGG‑GAACGGCGATCATGGCGa > 2:330021/1‑139 (MQ=255)
aTTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTc < 1:399823/139‑1 (MQ=255)
tAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCg < 2:1042912/139‑1 (MQ=255)
aTGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccacc > 2:579692/1‑139 (MQ=255)
cGTTTCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCt < 1:854852/139‑1 (MQ=255)
gTTACCCGAGCTGGCGTGGCTTAATACCATTCCGGGGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTg < 1:330021/139‑1 (MQ=255)
ttAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGG‑‑‑CACCACCACCTGGTTCGTTGGGCAGGCGGCGCata < 2:641266/139‑1 (MQ=255)
ttCCGGTGATTGGCGCGAAGCTGTATGCCGCCTGGCCCAAATGGGCGGATAC‑‑GGGGGGTG‑CCGACGATCATGGCGAAAGTCCGCCCTTATATTGGCACAACCACCACCTGGTTCGTTGGGCAGCCGGCGCATTTccgac > 2:916172/1‑135 (MQ=255)
ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg < 1:1098821/137‑1 (MQ=255)
ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg > 2:1098821/1‑137 (MQ=255)
tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt < 2:82506/132‑1 (MQ=255)
tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt > 1:82506/1‑132 (MQ=255)
tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt > 1:482218/1‑139 (MQ=255)
gcgcGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGc > 2:639801/1‑139 (MQ=255)
aGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGc < 1:1220865/37‑1 (MQ=37)
aGGCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGc > 2:1220865/1‑37 (MQ=37)
gCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCa > 2:1253765/1‑40 (MQ=38)
gCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCa < 1:1253765/40‑1 (MQ=255)
gCTGGCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtc > 1:486005/1‑139 (MQ=255)
ggCACAACTTGCTGGATATGGGGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTtctc > 2:1097853/1‑139 (MQ=255)
ggCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTc > 1:820605/1‑48 (MQ=255)
ggCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTc < 2:820605/48‑1 (MQ=39)
ggCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct > 2:369512/1‑139 (MQ=255)
ggCACAACTTGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct > 2:962908/1‑139 (MQ=255)
tGCTGGATATG‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGAGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGccc < 2:451888/139‑1 (MQ=255)
|
CGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGG‑G‑ACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC > NC_000913/1769357‑1769599
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
