Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F6 I0 R1
|
31 |
40.7 |
1457906 |
90.0% |
1312115 |
147.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,817,170 |
C→A |
100% |
E112* (GAA→TAA) |
yqaB ← |
fructose‑1‑P and 6‑phosphogluconate phosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,817,170 | 0 | C | A | 100.0%
| 88.9
/ ‑6.7
| 34 | E112* (GAA→TAA) | yqaB | fructose‑1‑P and 6‑phosphogluconate phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (14/18); minor base G (1/0); total (15/19) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCG > NC_000913_3_hsa_tpiA/2817029‑2817312
|
gTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAACGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGCTGGCGCTTTACCTcccc > 2:474930/1‑149 (MQ=255)
cccATATGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCTGGAGCGAGGATGGTGTTAATATAATGGCGCAATCCCTGGTGAGCCTGCAACTCCTCAGCGATGCCGCTTTAACTCCCCGTTCCttc > 2:357540/1‑147 (MQ=255)
cccATACGCTGCGCGCCAAACAAAAATGTGTTTGGCGGGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTAc < 2:275642/149‑1 (MQ=255)
cccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTAc < 2:111068/149‑1 (MQ=255)
ccATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTGGACGTGATCGGCAGCGACGACGGCGTCAAAATACTGGCGTCATGCCAGGGCAGCCACCAATGCCCCACCGATGGCGCGTTGCCTCCCCGGTCCtcta > 2:141037/1‑146 (MQ=255)
tACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGCTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTACCCGTTCCTACAGcc > 2:727751/1‑149 (MQ=255)
agcggACAACAAAAATGGGCCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGc < 2:105801/148‑1 (MQ=255)
caacaaAAATGGGTCTGGCGCGGGTTTATTGTGTTTGACGTGATCGGCACCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCgacgac < 1:418421/149‑1 (MQ=255)
aTGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCaa < 2:646307/149‑1 (MQ=255)
tGGGGCGGTGTTATGGTGTGTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTca < 2:318282/149‑1 (MQ=255)
gcgcGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTcacc > 2:433483/1‑149 (MQ=255)
tgaTGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTCAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATcaac < 2:301446/147‑1 (MQ=255)
tGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCACCGTTCCGACATCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAag > 1:21347/1‑149 (MQ=255)
ggTGTTTGACGTGATCGGCAGCGACGACGGCGTCTAAACAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAaga > 1:539508/1‑149 (MQ=255)
gtgtTTGACGTGATCGGCAGCGACGACGGCGACAAAATAATGGCGTAATCCCAGGGTAGCCAGCAATGCCGCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAagag > 1:292792/1‑149 (MQ=255)
tttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGaa > 1:522856/1‑149 (MQ=255)
ttGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAg < 1:35969/149‑1 (MQ=255)
gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAAc > 2:537927/1‑117 (MQ=255)
gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAAc < 1:537927/117‑1 (MQ=255)
gATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCg > 1:200701/1‑149 (MQ=255)
gCGACGACGGAGTCAAAATACTGGCGTAATGCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCAGTTCGACGCTATc > 1:708987/1‑149 (MQ=255)
gacgacGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCca < 2:701342/149‑1 (MQ=255)
agggCGTAATCCCGGGTGCGCGAGCAATGCCTCAGCGATGGCGCTTTAACTCACCGTTCCTACCGCCATTGGGCGACGACAATGCCAAATTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTg < 2:182296/147‑1 (MQ=255)
gggTAACCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCGACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCtt < 2:5923/147‑1 (MQ=255)
tCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGttt > 2:398697/1‑149 (MQ=255)
ggaaTGTCTCAGCGGTGGAGCTGTAAGTCCGCGTTCCTAGAGCCATTGGGGGACGACGATGGCAACGTTTCACCACAGCAGCAAGAGGAAGCGGTTCGACGCGATCCAGCATCGTACTTCTTTCTTCTTCTGTTTTTTCACGCGCGAAc < 2:351904/147‑1 (MQ=255)
gCAATGCCTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAAc < 1:605749/149‑1 (MQ=255)
cTCAGCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATCCTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCg > 2:419694/1‑149 (MQ=255)
gCGATGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTc < 1:713024/149‑1 (MQ=255)
ggtGGCGTCTTAACTCTCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCCCCACATCAACTAGAGGAAGCTGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCga < 2:240596/147‑1 (MQ=255)
gATGTCGCTTTTACGCCCGGATGCGAACGCCATTGGACGACTACCATGCCAACTTTTCACCACATCAACAAGAGCGCGCGGTTCGACGCTATTCAGCAGCATGCTTCTTACTGCTTCGGTGTTTTCACGCGCTAACGCATGCGGGTCga < 2:109501/149‑1 (MQ=255)
aTGGCGCTTTAACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCTACGCTCTCCAGCAGAATACGTCTGACTTCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCgag > 1:57224/1‑149 (MQ=255)
cgcTTTAACTCCCCGTTCCGAGCGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGGTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGTGCTAACGCAGGCGGGTCGAGATCg < 1:629437/149‑1 (MQ=255)
cgcTTTAACTCCCCGGTCCGACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGGTGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTATCGCATGCGGGTCGAGATCg < 2:314753/149‑1 (MQ=255)
|
GTTGCACGCCCATACGCTGCGCGCACAACAAAAATGTGTCTGGCGCGGGTTTATGGTGTTTGACGTGATCGGCAGCGACGACGGCGTCAAAATAATGGCGTAATCCCAGGTGCGCCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCG > NC_000913_3_hsa_tpiA/2817029‑2817312
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
